Sensitive and rapid detection methods for rare earth elements (REEs), including lanthanides (Lns), will facilitate the mining and recovery of these elements. Here, we innovated a rapid, highly selective and sensitive fluorescence detection method for Lns, based on Hans-Lanmodulin, a newly discovered protein with high selectivity and binding affinity for rare earth elements. By labelling the fluorescein moiety FITC onto Hans-Lanmodulin, named as FITC-Hans-LanM.
View Article and Find Full Text PDFExtracting uranium from water is crucial for environmental protection and the sustainable nuclear power industry. However, high-efficiency extraction and mild desorption condition still poses significant challenges. Herein, a polyoxometalate-based metal-organic framework (POMOF) for high-performance uranium extraction is prepared by in situ confined encapsulating H[PWO] (PW) into MIL-101(Cr).
View Article and Find Full Text PDFEffective uranium extraction from water is essential for the development of nuclear power industry and the protection of human health and environment. Nevertheless, it still remains challenging to realize efficient and cost-effective uranium extraction. Herein, a fast and simple method for the direct fabrication of novel functionalized hydrogen-bonded organic superstructures via molecular self-assembly is reported.
View Article and Find Full Text PDFACS Appl Mater Interfaces
October 2023
Highly selective capture of cesium (Cs) from complex aqueous solutions has become increasingly important owing to its (Cs) indispensable role in some cutting-edge technologies and the environmental mobility of radioactive nuclide (Cs) from nuclear wastewater. Herein, we report the development of cation-intercalated lamellar MoS as an effective Cs adsorbent with the advantages of facile synthesis and highly tunable layer spacing. Two types of cations, including Na and NH, were employed for the intercalations between adjacent layers of MoS.
View Article and Find Full Text PDFACS Appl Mater Interfaces
August 2023
ACS Appl Mater Interfaces
April 2021
Exploiting precious-metal-free and high-activity oxygen evolution reaction (OER) electrocatalysts has been in great demands toward many energy storage and conversion processes, for example, carbon dioxide reduction, metal-air batteries, and water splitting. In this study, the simple solid-state method is employed for coupling Ni (electron donors) with lower-Fermi-level MoO or WO (electron acceptors) into donor-acceptor ensembles with well-designed interfaces as robust electrocatalysts for OER. The resulting Ni/MoO and Ni/WO electrocatalysts exhibit smaller overpotentials of 287 and 333 mV at 10 mA cm as well as smaller Tafel slopes of 51 and 65 mV/dec, respectively, with respect to the single Ni, MoO, WO, and even the benchmark RuO in 1 M KOH.
View Article and Find Full Text PDFThree triphenylamine-based polyaminal networks (TMPs) with monodispersed ultramicropore (about 0.54 nm) and abundant doped-nitrogen (up to 42.88 wt%) are successfully prepared through the direct polycondensation of triphenylamine-based aldehydes with melamine.
View Article and Find Full Text PDFIn this paper, we described a temperature responsive nano-system that can regulate activity of enzyme with different temperature. Temperature responsive polymer poly(N-isopropylacrylamide) (PNIPAAm), with low critical solution temperature of 32°C, was synthesized with thiol modification. PNIPAAm and thrombin aptamer were co-functionalized on the surface of gold nanoparticles for effective regulation of thrombin activity with different temperature.
View Article and Find Full Text PDFIn the protein separation, adsorption and desorption of target protein have been using different buffer condition. Different buffer will change the structure and activity of target protein in some cases. This work describes the use of different wavelength light for remote regulation of adsorption and desorption of target protein in the same buffer solutions.
View Article and Find Full Text PDFRapid and sensitive detection of thrombin has very important significance in clinical diagnosis. In this work, bare magnetic iron oxide nanoparticles (magnetic nanoparticles) without any modification were used as fluorescence quenchers. In the absence of thrombin, a fluorescent dye (CY3) labeled thrombin aptamer (named CY3-aptamer) was adsorbed on the surface of magnetic nanoparticles through interaction between a phosphate backbone of the CY3-aptamer and hydroxyl groups on the bare magnetic nanoparticles in binding solution, leading to fluorescence quenching.
View Article and Find Full Text PDFIntroduction: Mogroside V is the main effective ingredient of Siraitia grosvenorii used as a natural sweet food as well as a traditional Chinese medicine. The sample pre-treatment prior to chromatographic analysis requires large amounts of toxic organic solvents and is time consuming.
Objective: To develop an effective and simple method for extracting and determining mogroside V of Siraitia grosvenorii.
J Colloid Interface Sci
April 2013
A stable drug carrier has been prepared by covalently coating magnetic nanoparticles (MNPs) with PEO-PPO-PEO block copolymer Pluronic P85. The particles were characterized by TEM, XRD, DLS, VSM, FTIR, and TGA. A typical product has a 15 nm magnetite core and a 100 nm hydrodynamic diameter with a narrow size distribution and is superparamagnetic with large saturation magnetization (57.
View Article and Find Full Text PDFAppl Biochem Biotechnol
December 2012
This study presents a novel and integrated preparation technology for nattokinase functional food, including strain screening, fermentation, separation, and encapsulation. To rapidly screen a nattokinase-productive strain, PCR-based screening method was combined with fibrinolytic activity-based method, and a high productive strain, Bacillus subtilis LSSE-22, was isolated from Chinese soybean paste. Reduction of poly-γ-glutamic acid (γ-PGA) concentration may contribute to separation of nattokinase and reduction of late-onset anaphylaxis risk.
View Article and Find Full Text PDFInteraction between PEO-PPO-PEO copolymers and a hexapeptide, growth hormone releasing peptide-6 (GHRP-6), was investigated by NMR to study the potential use of the copolymers in peptide drug delivery. (1)H NMR and nuclear Overhauser effect spectroscopy (NOESY) measurements determined that PO methyl protons interacted with methyl protons of the Ala moiety, aromatic protons of the Trp moiety, and some of the Phe aromatic protons. The Lys moiety and part of the Phe moiety entered the hydrophilic EO environment via hydrogen bonding.
View Article and Find Full Text PDFHexavalent chromate reductase was characterized and was found to be localized in the cytoplasmic fraction of a chromium-resistant bacterium Pannonibacter phragmitetus LSSE-09. The Cr(VI) reductase activity of cell-free extract (S₁₂) was significantly improved by external electron donors, such as NADH, glucose, acetate, formate, citrate, pyruvate, and lactate. The reductase activity was optimal at pH 7.
View Article and Find Full Text PDFSilver nanoparticles (AgNPs) were obtained by solar irradiation of cell-free extracts of Bacillusamyloliquefaciens and AgNO3. Light intensity, extract concentration, and NaCl addition influenced the synthesis of AgNPs. Under optimized conditions (solar intensity 70,000 lx, extract concentration 3 mg/mL, and NaCl content 2 mM), 98.
View Article and Find Full Text PDFThe influence of pH value on gold nanoparticle production in the presence of Pluronic stabilizers is systematically investigated. The reactions are studied as a function of pH and at fixed concentrations of the two reactants, HAuCl(4) and P123 block copolymer. Results indicate that the reaction pathway during the nanoparticle formation can be controlled by varying pH.
View Article and Find Full Text PDFCr(VI) was efficiently reduced to Cr(III) by Pannonibacter phragmitetus LSSE-09 encapsulated in liquid-core alginate-carboxymethyl cellulose capsules under alkaline conditions. Taking into account the physical properties of the capsules, the activity of encapsulated cells, and total Cr(III) concentration in the supernatant, optimal conditions (0.5% w/v sodium alginate; 2% w/v sodium carboxymethyl cellulose; 0.
View Article and Find Full Text PDFA novel cell separation and immobilization method for Cr (VI)-reduction under alkaline conditions was developed by using superparamagnetic Fe(3)O(4) nanoparticles (NPs). The Fe(3)O(4) NPs were synthesized by coprecipitation followed by modification with sodium citrate and polyethyleneimine (PEI). The surface-modified NPs were monodispersed and the particle size was about 15 nm with a saturation magnetization of 62.
View Article and Find Full Text PDFIn order to investigate the effect of PEO-PPO-PEO copolymers on the glutathione (GSH)/glutathione-S-transferase (GST) detoxification system, interaction between the copolymers and GSH is studied by NMR measurements. Selective rotating-frame nuclear Overhauser effect (ROE) experiment confirms that glutamyl (Glu) α-H of GSH has spatial contact with EO methylene protons. Spin-lattice relaxation times of GSH Glu α-H show a decrease when PEO-PPO-PEO copolymers are added, and the decrease is greater with copolymers possessing more EO units.
View Article and Find Full Text PDFNewly large-pore magnetic mesoporous silica nanoparticles (MMSNPs) with wormhole framework structures were synthesized for the first time by using tetraethyl orthosilicate as the silica source and amine-terminated Jeffamine surfactants as template. Iminodiacerate was attached on these MMSNPs through a silane-coupling agent and chelated with Cu(2+). The Cu(2+)-chelated MMSNPs (MMSNPs-CPTS-IDA-Cu(2+)) showed higher adsorption capacity of 98.
View Article and Find Full Text PDFJ Colloid Interface Sci
October 2010
pH- and temperature-responsive polymeric drug carriers based on Chitosan oligosaccharide (CSO)-g-Pluronic copolymers were successfully synthesized for Doxorubicin (DOX) controlled release. The critical aggregation concentration of CSO-g-Pluronic is 0.035 mg/mL at 25 degrees C.
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