Matrix Metalloproteinase-3 -1171 5A/6A Polymorphism (rs35068180) is Associated with Risk of Periodontitis.

Matrix metalloproteinase-3 (MMP3) plays a key role in tissue degradation in periodontitis. The relationship between the MMP3 -1171 5A/6A polymorphism (rs35068180) and periodontitis has been widely studied. However, existing studies have yielded contradictory results.

[Histological changes after the recontruction of tongue defect with rectus abdominis musculoperitoneal flap].

Objective: To observe the cellular morphological and histological changes of the reconstructed tongue defect by rectus abdominis musculoperitoneal flap of dogs with or without nerve.

Methods: 12 Beagle dogs were randomly divided into two groups. Group A made rectus abdominis musculoperitoneal flap with the intercostal nerve while group B without the intercostal nerve.

N-heterocyclic carbene (NHC)-catalyzed highly diastereo- and enantioselective oxo-Diels-Alder reactions for synthesis of fused pyrano[2,3-b]indoles.

A chiral N-heterocyclic carbene (NHC)-catalyzed Diels-Alder reaction of 2-oxoindolin-3-ylidenes and α-chloroaldehydes was developed for the synthesis of fused pyrano[2,3-b]indoles in good to excellent yields (up to 99%) with high cis-diastereoselectivities (>99:1 dr) and enantioselectivities (up to >99% ee).

[Expression of tumor necrosis factor in placenta tissue of pregnant rats with chronic peridontitis].

Objective: To examine the expression of tumor necrosis factor in placenta of pregnant rats with chronic periodontitis.

Methods: Twenty Wistar female rats were randomly divided into two groups, control (n = 8) and experimental group (n = 12). The periodontitis model was established in the experimental group.

[Establishment and analysis of chronic periodontitis and atherosclerosis model in Wistar rat].

Objective: To investigate the possible correlation between atherosclerosis and chronic periodontitis by establishing an animal model of chronic periodontitis and atherosclerosis in Wistar rat.

Methods: Sixty male Wistar rats were divided into four groups: A (control group), B (chronic periodontitis group), C (atherosclerosis group), D (chronic periodontitis accompany with atherosclerosis group). Every group was accepted the corresponding treatment.

[The correlation between interleukin-1 receptor antagonist genotype and coronary heart disease in patients with chronic periodontitis].

Purpose: To investigate the correlation between interleukin-1 receptor antagonist (IL-1ra) genotype and chronic periodontitis in patients with coronary heart disease.

Methods: Genomic DNA was obtained from buccal swabs in 86 patients with coronary heart disease,of which 45 patients with chronic periodontitis, the other without chronic periodontitis. Genotypes of IL-1RN intron 2 VNTR were analyzed by SSP-PCR method.

[Detection of periodontal pathogenic bacteria DNA in coronary atheromatous plaques from patients underwent coronary artery bypass graft].

Objective: The aim of this study was to observe the coexistence of periodontal bacteria DNA (Actinobacillus actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia, Tannerella. forsythensis) in coronary atheromatous plaques and subgingival plaques in patients scheduled for coronary artery bypass graft.

Methods: Coronary atheromatous plaque and subgingival plaque samples were obtained from 51 patients and examined by polymerase chain reaction (PCR) technique with specific primers for periodontal bacteria.

[Detection of periodontal pathogens in coronary atherosclerotic plaques].

Objective: To explore the possible correlation between coronary heart disease and periodontitis.

Methods: Subgingival plaque samples and coronary atherosclerotic plaques were harvested from a total of 31 patients with periodontitis who scheduled for coronary artery bypass surgery. The bacteria DNA was obtained from subgingival plaque samples and coronary atherosclerotic plaques using the chelex-100 method.

[Effects of xipayi mouth rinse on the DNA synthesis and change of cell cycles of human gingival fibroblast induced by lipopolysaccharide].

Objective: To observe the effects of xipayi mouth rinse on the DNA synthesis and change of cell cycles of human gingival fibroblast (HGF) induced by lipopolysaccharide(LPS).

Methods: HGF was stimulated with LPS at 25 mg/L. Flow cytometry was used to examine the effect of xipayi mouth rinse at 25 mg/L on the DNA synthesis and change of HGF cell cycles.

[Inhibitory effect of xipayi mouth rinse on the secretion of IL-6 from human gingival fibroblast induced by lipopolysaccharide].

Objective: To observe the effects of xipayi mouth rinse of different concentrations on the activities of interleukin-6 (IL-6) from human gingival fibroblast (HGF) induced by lipopolysaccharide (LPS).

Methods: HGF was stimulated with LPS at 25 g/mL, and the radioimmunoassay (RIA) was used to examine the effect of xipayi mouth rinse at 12.5 approximately 200 g/mL on the secretion of IL-6 in the supernatant of the cell culture.

[Study on the correlation between coronary heart disease and chronic periodontitis].

Objective: To explore the correlation between angiographically-defined coronary heart disease (CHD) and chronic periodontitis (CP).

Methods: 277 cases with CHD (case group) and another 238 with no agiographic evidence of CHD (control group) were compared on their traditional cardiovascular risk factors, as: oral health status and probing depth, clinical attachment level, gingival recession, as well as number of missing teeth. Other related risk factors of CHD were included in a stepwise logistic regression analysis.

[The correlation between polymorphism at position -511C/T in the promoter region of interleukin 1B and the severity of coronary heart disease].

Objective: To investigate the correlation between polymorphism at position -511C/T in the promoter region of interleukin 1B (IL1B) and the severity of coronary heart disease (CHD).

Methods: The polymerase chain reaction-restriction fragment length polymorphism technique was applied to analyze the polymorphisms of IL1B -511C/T in 127 patients with CHD and 152 controls. And the serum level of lipoproteins was detected by enzymology method.

[The association between gingival cervicular fluid Interleukin-6 level, serum estradiol status and periodontitis in Uyghur postmenopausal women].

Purpose: The purpose of this investigation was to analyze by cross-sectional design the association between GCF IL-6 level, serum E2 level and common clinical measurements of periodontitis in Uyghur postmenopausal women.

Methods: 79 subjects within 5 years after menopause were enrolled in the study. 30 GCF samples were harvested from healthy sites, 49 GCF samples from chronic periodontitis sites.

[The association between interleukin-1 receptor antagonist genotype and chronic periodontitis of Uighur patients].

Objective: To investigate interleukin-1 receptor antagonist (IL-1ra) genotype and its association with the susceptibility of chronic periodontitis in Uighur patients of Xinjiang.

Methods: Genomic DNA was obtained from buccal swabs of 41 subjects with severe chronic periodontitis (CP), 43 subjects with moderate CP, 49 subjects with mild CP and 92 ethnically matched healthy control individuals. Genotypes of IL-1RN intron 2 VNTR was analyzed by SSP-PCR method.