Influence of surface treatments on highly translucent zirconia: Mechanical, optical properties and bonding performance.

Objectives: Highly translucent yttria-stabilized zirconia (YSZ) has become more popular due to its enhanced aesthetics. This study aimed to evaluate the influence of traditional air abrasion and a new etching and cleaning agent, Multi Etchant, on the mechanical performance, optical properties, and bond strength of highly translucent zirconia.

Methods: Specimens of 6YSZ, 5YSZ, 4YSZ&5YSZ, and conventional 3YSZ were fabricated and underwent different surface treatments, including as milled, air abrasion, and Multi Etchant.

"Cation-Recognition" Effect of 2D Nanochannels in Graphene Oxide Membranes Intercalated with Ionic Liquid for High Desalination Performance.

Water and ion transport in nanochannels is crucial for membrane-based technology in biological systems. 2D materials, especially graphene oxide (GO), the most frequently used as the starting material, are ideal building blocks for developing synthetic membranes. However, the selective exclusion of small ions while maintaining in a pressured filtration process remains a challenge for GO membranes.

Preimplantation genetic testing for structural rearrangements by genome-wide SNP genotyping and haplotype analysis: a prospective multicenter clinical study.

Background: Preimplantation genetic testing for chromosomal structural rearrangements (PGT-SR) has been widely utilized to select euploid embryos in patients carrying balanced chromosomal rearrangements (BCRs) by chromosome copy number analysis. However, reliable and extensively validated PGT-SR methods for selecting embryos without BCRs in large-cohort studies are lacking.

Methods: In this prospective, multicenter, cohort study, carriers with BCRs undergoing PGT-SR were recruited across 12 academic fertility centers within China.

Nanoconfined electrostatic interaction for efficient anion sieving in graphene oxide membranes.

Controllable ion transport and precise ion sieving are crucial for sustainable water treatment and resource recovery. 2D materials, including graphene oxide (GO) with tunable nanochannels, are emerging as ideal material platforms to develop ion sieving membranes. However, accurate ion sieving remains challenging due to the swollen and enlarged interlayer spacing of GO membranes in aqueous solution, resulting in the non-selective of small ions.

Predicting the Potential Distribution of L. (Wild Vegetable) in China Under Different Climate Change Scenarios.

Global climate change has a main impact on the distribution of plants. L. is economically valuable, making it necessary to predict the impact of climate change on its distribution.

Mapping multi-regional functional connectivity of astrocyte-neuronal networks during behaviors.

Significance: Diverse behaviors rely on coordinated activity and multi-regional functional connectivity within astrocyte-neuronal networks. However, current techniques for simultaneously measuring astrocytic and neuronal activities across multiple brain regions during behaviors remain limited.

Aim: We propose a multi-fiber solution that can simultaneously record activities of astrocyte-neuronal networks across multiple regions during behaviors.

Malignant pleural effusion facilitates the establishment and maintenance of tumor organoid biobank with multiple patient-derived lung tumor cell sources.

The Patient-Derived Organoids (PDOs) has demonstrated significant potential in personalized medicine. However, the initial establishment of lung cancer organoids (LCOs), and timely therapeutic recommendations face several challenges. Particularly, the current culture systems have not yet achieved the capability to long-term cultivation of all lung tumor sample sources, including malignant pleural effusion (MPE), which poses significant barriers to the rapid clinical translation of PDOs.

Fullerenols as efficient ferroptosis inhibitor by targeting lipid peroxidation for preventing drug-induced acute kidney injury.

Acute kidney injury (AKI) is characterized by rapid and significant deterioration of renal function over a short duration with high mortality. However, the intricate pathophysiological mechanisms underlying AKI have hindered the development of effective therapeutic strategies. Recent research has highlighted the crucial role of ferroptosis in the pathogenesis of AKI and has identified it as a promising therapeutic target.

Effect of staining solutions on color and translucency stability of resin-composite computer-aided design and computer-aided manufacturing blocks.

Background: The purpose of this study was to investigate the color difference (ΔE) and translucency changes (ΔTP) of resin-composite computer-aided design and computer-aided manufacturing blocks after exposure to staining solutions.

Methods: A total of 250 rectangular specimens (1.0-mm thick) were prepared from 4 resin-based composites (Brilliant Crios [Coltène Whaledent], Lava Ultimate [3M], Hyramic [Upcera], and Shofu HC [Shofu]) and a polymer-infiltrated ceramic network material (Vita Enamic [Vita Zahnfabrik]).

Analyzing the impact of perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) on the reproductive system using network toxicology and molecular docking.

Growing evidence suggests that perfluorinated compounds (PFCs) contribute to reproductive toxicity, with perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) being the most extensively studied. These chemicals are known to lower testosterone levels and compromise the integrity of the blood-testis barrier. However, the specific mechanisms of their reproductive toxicity remain largely unknown due to research limitations.

Characterization and fine mapping of () mutant in sorghum [ (L.) Moench].

Unlabelled: The sorghum inflorescence is consisted of sessile (SS) and pedicellate spikelets (PS). Commonly, only SS could produce seeds and each spikelet produces one single seed. Here, we identified a sorghum mutant, named (), which can produce twin seeds in each pair of glumes.

DMF-Bimol: Counting mRNA and Protein Molecules in Single Cells with Digital Microfluidics.

Analyzing single-cell protein and mRNA levels yields invaluable insights into cellular functions and the intricacies of biologically heterogeneous systems. Current joint mRNAs and protein analysis methodologies suffer from relative quantification, low sensitivity, possible background interference, and tedious manual manipulation. Therefore, we propose DMF-Bimol that leverages addressable digital microfluidics to automate digital counting of single-cell mRNA and protein based on proximity ligation assay (PLA) and one-step RT-droplet digital PCR (RT-ddPCR).

Reconstructing tumor clonal heterogeneity and evolutionary relationships based on tumor DNA sequencing data.

The heterogeneity of tumor clones drives the selection and evolution of distinct tumor cell populations, resulting in an intricate and dynamic tumor evolution process. While tumor bulk DNA sequencing helps elucidate intratumor heterogeneity, challenges such as the misidentification of mutation multiplicity due to copy number variations and uncertainties in the reconstruction process hinder the accurate inference of tumor evolution. In this study, we introduce a novel approach, REconstructing Tumor Clonal Heterogeneity and Evolutionary Relationships (RETCHER), which characterizes more realistic cancer cell fractions by accurately identifying mutation multiplicity while considering uncertainty during the reconstruction process and the credibility and reasonableness of subclone clustering.

Blastocysts originated from oocytes with smooth endoplasmic reticulum aggregates have a reduced euploidy rate: a retrospective cohort study.

Research Question: Does the presence of smooth endoplasmic reticulum aggregates (SERa) in oocytes adversely impact the euploidy rate of subsequent blastocysts?

Design: We performed a retrospective cohort study with 671 young patients (< 38 years) undergoing their first preimplantation genetic testing for aneuploidy (PGT-A) between January 2019 and October 2022 at a reproductive medical center of university affiliated teaching hospitals in China. Cycles were categorized as either SERa(+) cycles (containing at least one SERa(+) oocyte) or SERa(-) cycles (all oocytes without SERa). In SERa(+) cycles, oocytes were further subdivided into the SERa(+) oocyte group and the sibling SERa(-) oocyte group, comprising oocytes with normal morphology.

Masking ability of CAD-CAM resin-matrix ceramics with different translucencies and thicknesses combined with four cement shades against varying background colors when facing veneer restorations.

Background: To evaluate the comprehensive effect of translucency, thickness, cement shades, and background color on the masking ability of resin-matrix ceramic veneer restorations.

Methods: Resin-matrix ceramic specimens with 2 translucencies (LT, HT) and 3 thicknesses (0.5, 1.

DMF-ChIP-seq for Highly Sensitive and Integrated Epigenomic Profiling of Low-Input Cells.

Mapping genome-wide DNA-protein interactions (DPIs) provides insights into the epigenetic landscape of complex biological systems and elucidates the mechanisms of epigenetic regulation in biological progress. However, current technologies in DPI profiling still suffer from high cell demands, low detection sensitivity, and large reagent consumption. To address these problems, we developed DMF-ChIP-seq that builds on digital microfluidic (DMF) technology to profile genome-wide DPIs in a highly efficient, cost-effective, and user-friendly way.

Generation of a hiPSC line (TONGJIi001-A) from a 46,XX,ins(1;15)(p13.3; q22.31q26.1),inv(2)(p22.1p16.3),t(2;14)(q34;q12) infertility patient.

We have successfully derived a hiPSC line from PBMCs obtained from a 41-year-old infertile female. The patient's karyotype, as determined by Bionano OGM, revealed complex chromosomal rearrangements, including 46,XX,ins(1;15)(p13.3;q22.

A predictive risk-scoring model for survival prognosis of multiple myeloma based on gain/amplification of 1q21: Experience in a tertiary hospital in South-Western China.

Background: Chromosomal 1q gains and amplifications (+1q21) are frequently observed in patients with newly diagnosed multiple myeloma (NDMM). However, the interpretation of the high-risk (HR) prognostic implications stemming from 1q21 abnormalities remain challenging to implement effectively.

Methods: In a comprehensive analysis of 367 consecutive patients with symptomatic MM, we assessed the prognostic significance of +1q21 using FISH with a threshold of 7.

Novel Loss-of-Function Variants in Infertile Males Upgrade the Gene-Disease Clinical Validity Classification for and Male Infertility to Strong.

Male infertility affects approximately 7% of the male population, and about 15% of these cases are predicted to have a genetic etiology. One gene implicated in autosomal dominant male infertility, , encodes a protein critical for the synapsis of homologous chromosomes during meiosis I, resulting in impaired spermatogenesis. However, the clinical validity of the gene-disease pair was previously categorized as on the border of limited and moderate due to few reported cases.

Spurious dyslipidemia due to paraprotein in a patient with Waldenström macroglobulinemia.

Introduction: Serum lipid profiles play a crucial role in diagnosing and evaluating cardiovascular diseases. However, the presence of paraprotein can lead to inaccurate dyslipidemia results on automated analyzers.

Case Report: A 65-year-old woman whose combined concentrations of HDL-cholesterol (HDL-C) and LDL-cholesterol (LDL-C) consistently surpassed her total serum cholesterol levels over a period of three months presented with unusual lipid component detection.

Comparison of case-based and lecture-based learning in dental fluorosis diagnostic ability with visual analog scale assessment.

Objective: This study aimed to compare the impact of case-based learning (CBL) versus lecture-based learning (LBL) on dental students' clinical decision-making regarding DF severity using Visual Analog Scale (VAS) scoring.

Methods: Eighty first-year graduate dental students were randomly assigned to either the CBL (n = 38) or LBL (n = 42) groups. Both groups received instruction on DF diagnosis, with CBL involving small group sessions analyzing real cases and LBL involving traditional lectures.