Transcriptomic and metabolomic analysis reveal the cold tolerance mechanism of common beans under cold stress.

Background: Common bean (Phaseolus vulgaris L.) is a thermophilic crop, and exposure to cold stress can significantly impact their yield and quality. To elucidate the impact of cold stress on cold-tolerant 'Wei Yuan' (WY) and cold-sensitive 'Bai Bu Lao' (BBL) of common bean, the mechanism of cold tolerance was studied by physiological and biochemical and multi-omics analysis.

Dietary succinate supplementation alleviates DSS-induced colitis via the IL-4Rα/Hif-1α Axis.

Inflammatory bowel disease (IBD) remains a pressing global health challenge, necessitating novel therapeutic strategies. Succinate, a metabolite known for its role in type 2 immunity and tuft cell activation in the small intestine, presents its potential in IBD management. However, its impact on colonic inflammation has not been explored.

Multi-omics analyses of the gut microbiota and metabolites in children with metabolic dysfunction-associated steatotic liver disease.

The development and severity of metabolic dysfunction-associated steatotic liver disease (MASLD) in children are closely related to alterations of gut microbiota. This study aims to investigate changes in the gut microbiota signature and microbial metabolites in children with MASLD. We collected fecal samples from children and adolescents aged 6-16 years, and the presence of MASLD was diagnosed by ultrasound.

Tissue-specific extracellular matrix for the larger-scaled expansion of spinal cord organoids.

Spinal cord organoids (SCOs) are in vitro models that faithfully recapitulate the basic tissue architecture and cell types of the spinal cord and play a crucial role in developmental studies, disease modeling, and drug screening. Physiological cues are required for proliferation and differentiation during SCO culture. However, commonly used basement membrane matrix products, such as Matrigel®, lack tissue-specific biophysical signals.

Altered functional connectivity of thalamic subregions in premenstrual syndrome.

Purpose: This article aims to explore the relationship between the symptoms of PMS and changes of thalamic subregions.

Methods And Materials: 71 patients diagnosed with PMS and 81 healthy controls (HCs) were included in the study. Participant status was determined using the Daily Record of Severity of Problems (DRSP) scale.

Combined skin injury model from airblast overpressure and seawater immersion in rats: establishment, characterization, and mechanistic insights.

In maritime operations, individuals often face the threat of combined injury caused by airblast overpressure and seawater immersion. Airblast overpressure, induced by explosions, leads to significant internal damage despite the absence of visible open wounds. Seawater immersion exacerbates injuries due to its high osmolarity, microbial content, and thermal conductivity.

Associations between exposure to brominated flame retardants and hyperlipidemia risk in U.S. Adults.

Background: Environmental exposure to toxic brominated flame retardants (BFRs) has been confirmed to have detrimental effects on human health. The impact of serum BFRs on hyperlipidemia risk has not been sufficiently examined. Our objective is to identify both the individual and combined effects of serum BFRs on hyperlipidemia and to further investigate the most influential chemicals.

Rapid resolution of newly formed carotid free-floating thrombus through anticoagulation therapy.

Background: Carotid free-floating thrombi (CFFT) are uncommon but pose a high risk of embolic stroke. While vascular intervention is often favored, this case study demonstrates the potential of short-term anticoagulation, guided by thorough imaging, as an alternative treatment strategy.

Case Presentation: We present a patient with an acute ischemic stroke (NIHSS 15) due to middle cerebral artery (MCA) occlusion.

Discovery and identification of semaphorin 4D as a bioindicator of high fracture incidence in type 2 diabetic mice with glucose control.

Introduction: Bone fracture is increasing in patients with type 2 diabetes mellitus (T2DM) due to skeletal fragility. Most antidiabetics are expected to reduce the incidence of fracture in patients with T2DM, however the results are disappointing. Metformin and GLP-1 receptor agonists have a neutral or minor positive effect in reducing fractures.

NanoAbLLaMA: construction of nanobody libraries with protein large language models.

Introduction: Traditional methods for constructing synthetic nanobody libraries are labor-intensive and time-consuming. This study introduces a novel approach leveraging protein large language models (LLMs) to generate germline-specific nanobody sequences, enabling efficient library construction through statistical analysis.

Methods: We developed NanoAbLLaMA, a protein LLM based on LLaMA2, fine-tuned using low-rank adaptation (LoRA) on 120,000 curated nanobody sequences.

A J-Domain Protein J3 Antagonizes ABI5-BINDING PROTEIN2 to Regulate CONSTANS Stability and Flowering Time.

CONSTANS (CO) plays a vital role in activating the expression of the florigen FT during photoperiod-dependent flowering. The diurnal oscillation of CO protein abundance is strictly controlled by daylength and the circadian clock. We showed previously that ABI5-BINDING PROTEIN2 (AFP2) represses CO expression to delay flowering.

Alterations in PD-L1 succinylation shape anti-tumor immune responses in melanoma.

Tumors undergo metabolic reprogramming to meet the energetic, synthetic and redox demands essential for malignancy, often characterized by increased glycolysis and lactate production. However, the role of mitochondrial metabolism in tumor immunity remains unclear. The present study integrates spatial transcriptomics, bulk transcriptomics and proteomics, revealing a strong link between the metabolite succinyl-CoA and tumor immunity as well as the efficacy of anti-programmed cell death protein-1 (PD-1) therapy in patients with melanoma.

The role of the TREM receptor family in cardiovascular diseases: Functions, mechanisms, and therapeutic target.

The Triggering Receptor Expressed in the Myeloid Cells (TREM) family represents an emerging subgroup within the immunoglobulin superfamily, which includes key members such as TREM-1, TREM-2, TREM-3, TREM-like transcript-1 (TLT-1), TLT-2, and TLT-4. TREM-1 serves as a potent amplifier of immune responses, exacerbating atherosclerosis and myocardial injury by enhancing inflammatory reactions. In contrast, TREM-2 exerts protective effects by regulating lipid metabolism, mitigating inflammation, and promoting phagocytic activity, thereby attenuating cardiovascular damage.

Ultrasensitive terahertz metasensor integrated with EIT-like coupling and liquid flow micro-channels for organic solvent detection and identification.

Terahertz (THz)-based metasurface sensors offer potential and extensive application prospects for organic solvent detection. This study proposed a liquid sensor comprising an electromagnetically induced transparency (EIT)-like THz metasurface covered by a channel. The designed device can exploit variations in the EIT-like resonance peaks to distinguish between the concentrations of three types of organic solvents (ethanol(ET), acetone(AC), and isopropyl alcohol(IPA)).

Mobile phone tasks impair frontal dynamic stability and increase fall risk during obstacle crossing in young men.

Falls, a major cause of accidental deaths, are often caused by obstacles, particularly among young people who may trip in over half of cases. Although mobile phone use has been linked to impaired gait and balance, its effect on dynamic stability during obstacle crossing is not well understood. This study investigates the impact of mobile phone usage on dynamic stability and fall risk during obstacle-crossing movements and compares the effects of various mobile phone tasks on obstacle-crossing performance.

Imbalance of bladder neurohomeostasis by Myosin 5a aggravates diabetic cystopathy.

Background: Diabetic cystopathy (DCP) is linked to bladder nerve conduction disorders, with diabetes-induced neuropathy impairing nerve signal transmission and causing bladder dysfunction. Myosin 5a, vital for neuronal transport, has been linked to neurological disorders, though its role in DCP remains unclear. The objective of this study was to investigate whether Myosin 5a plays a potential regulatory role in Diabetic Cystopathy.

ZNF385A was identified as a novel colorectal cancer-related functional gene by analysis of the interaction and immune characteristics of oxidative stress and the inflammatory response.

Background: Recently, oxidative stress and inflammatory responses have been shown to directly impact tumor growth and the tumor microenvironment (TME). However, more research is necessary to fully understand the relationship between oxidative stress and inflammatory responses and colorectal cancer (CRC).

Methods: The FindCluster algorithm was used to extract CRC Single-cell RNA sequencing (scRNA-seq) data and identify tumor cell groupings.

Modular Synthesis of Bioactive Selenoheterocycles for Efficient Cancer Therapy via Electrochemical Selenylation/Cyclization.

A green, efficient, and environmentally friendly electrochemical strategy was developed for synthesizing a series of selenoheterocyclic compounds. The antitumor activities of these compounds were evaluated, revealing that compounds , , and demonstrated remarkable antitumor efficacy. These compounds effectively inhibited lung cancer by inducing cell apoptosis, causing DNA damage, and suppressing the progression of epithelial-mesenchymal transition.

Cercus Electric Stimulation Enables Cockroach with Trajectory Control and Spatial Cognition Training.

Cyborg insects are highly adaptable for detection and recognition assignments, achieved through the electrical stimulation of multiple organs and nerves to control their locomotion. However, it remains unclear whether these control strategies can promote memory formation in insects, thereby facilitating their training for recognition assignments. In this study, we employed a steering control strategy for cyborg insects in operant learning training of cockroaches in a T-maze.

Establishment and validation of a nomogram for predicting esophagogastric variceal bleeding in patients with liver cirrhosis.

Background: Patients with decompensated liver cirrhosis suffering from esophagogastric variceal bleeding (EGVB) face high mortality.

Aim: To investigate the risk factors for EGVB in patients with liver cirrhosis and establish a diagnostic nomogram.

Methods: Patients with liver cirrhosis who met the inclusion criteria were randomly divided into training and validation cohorts in a 6:4 ratio in this retrospective research.

Widely targeted metabolomics of different tissues in .

, a medicinal plant of the family, is known for its diverse bioactive compounds. This study employs widely targeted metabolomics to investigate the metabolic profiles of leaf, stem, and flower tissue from . Using ultra-performance liquid chromatography coupled with tandem mass spectrometry, we identified 1,946 metabolites across the three tissue types.

Recent advances and challenges in metal-based antimicrobial materials: a review of strategies to combat antibiotic resistance.

Despite the availability of a series of classical antibiotic drugs, bacterial infections continue to represent a significant and urgent threat to global human health. The emergence of drug-resistant bacteria and the slow pace of antibiotic development have rendered current treatment methods inadequate in meeting the clinical demands of bacterial infections. Consequently, there is an increasingly urgent and vital need for the development of safe, efficient, and alternative novel antimicrobial agents in the medical and healthcare field.

Prevalence and Risk Factors of Chemotherapy-Induced Oral Mucositis in 470 Children With Acute Lymphoblastic Leukemia.

Objective: To assess the prevalence and risk factors of chemotherapy-induced oral mucositis in 470 children with acute lymphoblastic leukemia in China, focusing on treatment-related risk factors.

Methods: This cross-sectional study included 470 children diagnosed with acute lymphoblastic leukemia in China from January 2020 to July 2022. Data were collected from electronic medical records, field investigations, and telephone follow-ups, covering sociodemographic characteristics, nutritional status, disease and treatment history, and microbiological factors.

Chemoradiation treatment with or without concurrent tumor-treating fields (TTFields) therapy in newly diagnosed glioblastoma (GBM) patients in China.

Background: Tumor-treating fields (TTFields) therapy and radiotherapy may have synergistic anti-glioma effect based on preclinical studies. The combination of chemoradiation therapy (CRT) with TTFields therapy has noticeably attracted clinicians' attention. This study aimed to provide insights into the clinical outcomes of patients with newly diagnosed glioblastoma who received either concurrent CRT and TTFields therapy or adjuvant TTFields therapy following CRT.

Ruthenium(II) polypyridyl complexes inhibit tumor growth through stimulating immune system to increase CD8 T cell.

In this work, we have carefully designed and synthesized two Ru(II) metal complexes: [Ru(phen)(HMPIP)](PF) (6a, where phen = 1,10-phenanthroline, HMPIP = 2-(2-hydroxy-3-methylphenyl-1H-imidazo[4,5-f][1,10]phenanthroline) and [Ru(bpy)(HMPIP)](PF) (6b, where bpy = 2,2'-bipyridine). Using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to explore the cytotoxicity of 6a and 6b towards HepG2, B16, A549, SGC-7901, HCT116 and non-cancer LO2. The complexes exhibited cytotoxicity activity against HepG2 cells.