Low-dose acrylamide induces mitochondrial autophagy and energy metabolism dysfunction in SH-SY5Y cells via BV2 microglial activation.

Acrylamide (ACR), a common environmental and food contaminant, can cause neurotoxicity and increase the risk of neurodegenerative diseases. While the neurotoxicity induced by high-dose ACR exposure in occupational workers is evident, the potential adverse effects of low-dose daily exposure remain a subject of debate. In this present study, we aim to explore whether low-dose ACR induces neurotoxicity based on the interactions between microglia and neurons.

Nanoengineered cargo with targeted gene editing modulated mitophagy of chondrocytes to alleviate osteoarthritis.

Mitochondrial dysfunction in chondrocytes is a key pathogenic factor in osteoarthritis (OA), but directly modulating mitochondria remains a significant challenge. This study is the first to verify a correlation between mitochondrial dysfunction and the downregulation of the gene in the cartilage of OA patients, highlighting the potential for regulating mitophagy gene modulation to alleviate OA. Consequently, we developed a chondrocyte-targeting CRISPR/Cas9-based gene-editing tool (FoxO3) and integrated it within a nanoengineered 'truck' (NETT, FoxO3-NETT).

Predicting Functional Surface Topographies Combining Topological Data Analysis and Deep Learning Across the Human Protein Universe.

Characterizing geometric and topological properties of protein structures encompassing surface pockets, interior cavities, and cross channels is important for understanding their functions. Our knowledge of protein structures has been greatly advanced by AI-powered structure prediction tools, with AlphaFold2 (AF2) providing accurate 3D structure predictions for most protein sequences. Nonetheless, there is a substantial lack of function annotations and corresponding functional surface topographical information.

Effects of Lamina-Chromatin Attachment on Super Long-Range Chromatin Interactions.

The interactions between chromatin and lamin proteins localized on the nuclear envelope play a crucial role in the three-dimensional (3D) organization of the genome. This study investigates the influence of lamin associated domains (LADs) on genome organization at the chromosome level using 3D polymer models of mouse embryonic fibroblasts (MEFs) and embryonic stem cells (mESCs). By integrating genome-wide LAD maps from DamID assays, we simulated chromatin conformations with and without LAD attachment to the nuclear envelope.

Deubiquitination of RIPK3 by OTUB2 potentiates neuronal necroptosis after ischemic stroke.

As a common and severe cerebrovascular disease, ischemic stroke casts a significant shadow over global health. Unfortunately, the mechanisms regulating neuronal death in the affected areas remain largely unclear. Here, we found that deletion of the deubiquitinating enzyme Otubain-2 (OTUB2) significantly alleviated ischemia-induced cerebral infarction and neurological deficits, accompanied by a reduction in neuronal loss, glial activation, and neuroinflammation.

The Guanylate Cyclase Soluble Subunit Alpha-1 Deficiency Impairs Angiogenesis in Zebrafishes and Mice: In Vivo and In Vitro Studies.

Moyamoya disease (MMD) is caused by abnormal vascular development. Guanylate cyclase soluble subunit alpha-1 (GUCY1A3) gene variation is verified as a crucial susceptible gene in MMD. In this study, we investigated the impact of GUCY1A3 on angiogenesis.

Overexpression of methionine sulfoxide reductase A alleviates acrylamide-induced neurotoxicity by mitigating lipid peroxidation and mitochondria-dependent apoptosis In vivo and In vitro.

Acrylamide (ACR) has garnered significant attention due to its neurotoxic effects. Oxidative stress, a key mechanism underlying ACR-induced neurotoxicity, is well-documented. Methionine sulfoxide reductase A (MsrA) plays a pivotal role in protecting various types of cells, including neuronal cells, against the effects of oxidative stress.

IGF2BP1/AIFM2 axis regulates ferroptosis and glycolysis to drive hepatocellular carcinoma progression.

Background: Hepatocellular carcinoma (HCC) is aggressive liver tumor that is the third leading cause of cancer death. Ferroptosis and glycolysis play key roles in HCC progression. Apoptosis-inducing factor mitochondria-associated 2 (AIFM2) in involved in regulating ferroptosis and glycolysis in cancers, but its role in HCC remains unclear.

Identification of Ferroptosis-Related Prognostic Models and FDFT1 as a Potential Ferroptosis Driver in Colorectal Cancer.

Aims: We aimed to develop Ferroptosis-Related Gene (FRG) signatures to predict overall survival (OS) along with disease-free survival (DFS) in individuals with colorectal cancer (CRC).

Background: Prediction of CRC prognosis is challenging. Ferroptosis constitutes a newly reported kind of cell death, and its association with CRC prognosis remains unexplored.

Synergy from the Stepped Hollow FeHCFe Nanocubes and the Dimorphic Polypyrrole for High-Performance Electrochemical Water Desalination.

Saline water desalination serves as an important route to increase freshwater supply, while capacitive deionization (CDI) has emerged as a promising technique to tackle this issue. To boost the successful application of CDI, development of advanced electrode materials is vital. Herein, we innovatively designed and synthesized a sophisticated Prussian blue/dimorphic polypyrrole composite for the hybrid CDI (HCDI).

Study on the Mechanism of the Leaves of Dimocarpus longan Lour. in the Management of Type 2 Diabetes based on Metabolomics.

Background: Diabetes mellitus (DM) is a chronic metabolic disease. The leaves of Dimocarpus longan Lour. (LYY), a well-known traditional Chinese medicine (TCM) with Guangxi national characteristics often used in simple recipes to treat DM has attracted increasing attention.

Serum Immune-Response Protein Biomarkers Based on Olink Technology for Diagnosis of Ischemic Stroke.

The immune response plays a crucial role in the treatment of ischemic stroke (IS). Our primary objective was to explore immune proteins related to stroke and to develop a noninvasive diagnostic panel. We used the high-throughput Olink immunoassay platform to quantitatively measure 92 proteins in the serum of 88 patients with IS and 88 controls.

Structural basis of differential gene expression at eQTLs loci from high-resolution ensemble models of 3D single-cell chromatin conformations.

Motivation: Techniques such as high-throughput chromosome conformation capture (Hi-C) have provided a wealth of information on nucleus organization and genome important for understanding gene expression regulation. Genome-Wide Association Studies have identified numerous loci associated with complex traits. Expression quantitative trait loci (eQTL) studies have further linked the genetic variants to alteration in expression levels of associated target genes across individuals.

Precursor-Induced Growth of Highly-Oriented Nanowire Arrays.

The unique optical, electrical, and thermal properties of 1D nanowires have sparked significant interest in growing high-quality 1D materials. Nanowire arrays and aligned growth offer scalability and maintain anisotropic properties, making them promising for research and applications. However, mass-producing high-quality nanowire arrays remains a challenge.

Semi-quantitative scoring criteria based on multiple staining methods combined with machine learning to evaluate residual nuclei in decellularized matrix.

The detection of residual nuclei in decellularized extracellular matrix (dECM) biomaterials is critical for ensuring their quality and biocompatibility. However, current evaluation methods have limitations in addressing impurity interference and providing intelligent analysis. In this study, we utilized four staining techniques-hematoxylin-eosin staining, acetocarmine staining, the Feulgen reaction and 4',6-diamidino-2-phenylindole staining-to detect residual nuclei in dECM biomaterials.

Unraveling the interaction of dissolved organic matter and microorganisms with internal phosphorus cycling in the floodplain lake ecosystem.

Internal nutrient cycling, especially phosphorus (P), is of great influence in lake eutrophication. Dissolved organic matter (DOM) and microorganisms are ubiquitous in the sediments and closely associated with P-cycling. However, the underlying interactions of DOM, microorganisms and P in floodplain lake area with different hydrological characteristics remain scarce.

Associations of early-onset coronary heart disease and genetic susceptibility with incident dementia and white matter hyperintensity: A prospective cohort study.

Background: The associations of early-onset coronary heart disease (CHD) and genetic susceptibility with incident dementia and brain white matter hyperintensity (WMH) remain unclear. Elucidation of this problem could promote understanding of the neurocognitive impact of early-onset CHD and provide suggestions for the prevention of dementia.

Objectives: This study aimed to investigate whether observed and genetically predicted early-onset CHD were related to subsequent dementia and WMH volume.

Identification of chemical constituents and absorbed components in rat serum of (L.) Jacq. by UPLC-Q-exactive-MS.

(L.) Jacq. has anti-inflammatory, analgesic, haemostatic and antioxidant effects, but its pharmacological components are still unclear.

Musical Pitch Perception and Categorization in Listeners with No Musical Training Experience: Insights from Mandarin-Speaking Non-Musicians.

Pitch is a fundamental element in music. While most previous studies on musical pitch have focused on musicians, our understanding of musical pitch perception in non-musicians is still limited. This study aimed to explore how Mandarin-speaking listeners who did not receive musical training perceive and categorize musical pitch.

Remodeling the Proinflammatory Microenvironment in Osteoarthritis through Interleukin-1 Beta Tailored Exosome Cargo for Inflammatory Regulation and Cartilage Regeneration.

Osteoarthritis (OA) presents a significant therapeutic challenge, with few options for preserving joint cartilage and repairing associated tissue damage. Inflammation is a pivotal factor in OA-induced cartilage deterioration and synovial inflammation. Recently, exosomes derived from human umbilical cord mesenchymal stem cells (HucMSCs) have gained recognition as a promising noncellular therapeutic modality, but their use is hindered by the challenge of harvesting a sufficient number of exosomes with effective therapeutic efficacy.

Predictive tool for the risk of hypothermia during laparoscopic gynecologic tumor resection.

Objective: Based on the machine learning algorithm, construct a hypothermia prediction model for gynecological tumor resection under laparoscopic general anesthesia.

Methods: This research conducted a retrospective analysis, gathering data from individuals who had undergone minimally invasive surgical procedures for gynecological tumors in a Chinese Hospital, ranging from June 2018 to August 2024. During this timeframe, a total of 308 cases were examined for analysis, with 70% of the cases allocated to the modeling dataset and the remaining 30% designated for the validation dataset.

Anchoring of Probiotic-Membrane Vesicles in Hydrogels Facilitates Wound Vascularization.

Inadequate vascularization significantly hampers wound recovery by limiting nutrient delivery. To address this challenge, we extracted membrane vesicles from (LMVs) and identified their angiogenic potential via transcriptomic analysis. We further developed a composite hydrogel system (Gel-LMVs) by anchoring LMVs within carboxylated chitosan and cross-linking it with oxidized hyaluronic acid through a Schiff base reaction.

An OMV-Based Nanovaccine as Antigen Presentation Signal Enhancer for Cancer Immunotherapy.

Antigen-presenting cells (APCs) process tumor vaccines and present tumor antigens as the first signals to T cells to activate anti-tumor immunity, which process requires the assistance of co-stimulatory second signals on APCs. The immune checkpoint programmed death ligand 1 (PD-L1) not only mediates the immune escape of tumor cells but also acts as a co-inhibitory second signal on APCs. The serious dysfunction of second signals due to the high expression of PD-L1 on APCs in the tumor body results in the inefficiency of tumor vaccines.

Establishment and internal validation of a model to predict the efficacy of Adalimumab in Crohn's disease.

Background: Clinically, the ability to distinguish which Crohn's disease patients can benefit from Adalimumab is limited.

Aims: This study aimed to develop a model for predicting clinical remission probability for Crohn's disease patients with Adalimumab at 12 weeks. The model assists clinicians in identifying which Crohn's disease patients are likely to benefit from Adalimumab treatment before starting therapy, thus optimizing individualized treatment strategies.

Achieving Narrowband and Stable Pure Blue Organic Light-Emitting Diodes by Employing Molecular Vibration Limited Strategies in the Extended π-Conjugated Indolocarbazole Skeleton.

B- and N-heterocyclic fluorophores have reveal promising efficiency in blue organic light-emitting diodes (OLEDs) with small full-width-at-half-maximum (FWHM). However, their structural determinants for spectral broadening and operating stability are still needed to be investigated in further. Herein, a novel multi-N-heterocycles Diindolo[3,2,1jk:3',2',1'jk]dicarbazole[1,2-b:4,5-b] (DIDCz) is proposed to manipulate the emission color toward pure blue region by extending π-conjugation of the N-π-N bridge.