Pyroptosis inhibition alleviates acute lung injury via E-twenty-six variant gene 5-mediated downregulation of gasdermin D.

Background: Acute lung injury (ALI) is a life-threatening condition characterized by excessive pulmonary inflammation, yet its precise pathophysiology remains elusive. Pyroptosis, a programmed cell death mechanism controlled by gasdermin D (GSDMD), has been linked to the etiology of ALI. This study investigated the regulatory functions of the transcription factor E-twenty-six variant gene 5 (ETV5) and GSDMD in ALI.

Experimental evolution of gene essentiality in bacteria.

Essential gene products carry out fundamental cellular activities in interaction with other components. However, the lack of essential gene mutants and appropriate methodologies to link essential gene functions with their partners poses significant challenges. Here, we have generated deletion mutants in 32 genes previously identified as essential, with 23 mutants showing extremely slow growth in the SK36 strain of .

High-throughput characterization of the influence of Streptococcus sanguinis genes on the interaction between Streptococcus sanguinis and Porphyromonas gingivalis.

Porphyromonas gingivalis is a keystone pathogen in periodontitis, and Streptococcus sanguinis is an abundant oral commensal bacterium associated with periodontal health. However, the interaction between P. gingivalis and S.

A novel infective endocarditis virulence factor related to multiple functions for bacterial survival in blood was discovered in .

We identified the role of a conserved hypothetical protein (SSA_0451) in that is involved in the virulence of infective endocarditis. An whole blood killing assay and rabbit endocarditis model studies revealed that the SSA_0451 mutant (ΔSSA_0451) was significantly less virulent than the wild-type (SK36) and its complementation mutant (ΔSSA_0451C). The mechanism underlying the SSA_0451 mutant's reduced virulence in infective endocarditis was evidentially linked to oxidative stress and environmental stress.

Interface reconstruction of MXene-TiC doped CeO nanorods for remarked photocatalytic ammonia synthesis.

Prospective photocatalytic ammonia synthesis process has received more attentions but quite challenging with the low visible light utilization and weak N molecule absorption ability around the photocatalysts. Herein, interface reconstruction of MXene-TiC/CeO composites with high-concentration active sites through the carbon-doped process are presented firstly, and obvious transition zones with the three-phase reaction interface are formed in the as-prepared catalysts. The optimal co-doped sample demonstrates an excellent photo response in the visible light region, the strongest chemisorption activity and the most active sites.

Accelerated Charge Transfer through Interface Chemical Bonds in MoS/TiO for Photocatalytic Conversion of Lignocellulosic Biomass to H.

Solar photocatalytic H production from lignocellulosic biomass has attracted great interest, but it suffers from low photocatalytic efficiency owing to the absence of highly efficient photocatalysts. Herein, we designed and constructed ultrathin MoS-modified porous TiO microspheres (MT) with abundant interface Ti-S bonds as photocatalysts for photocatalytic H generation from lignocellulosic biomass. Owing to the accelerated charge transfer related to Ti-S bonds, as well as the abundant active sites for both H and OH generation, respectively, related to the high exposed edge of MoS and the large specific surface area of TiO, MT photocatalysts demonstrate good performance in the photocatalytic conversion of α-cellulose and lignocellulosic biomass to H.

A Predictive Model Based on the FBXO Family Reveals the Significance of Cyclin F in Hepatocellular Carcinoma.

Objective: The F-box protein (FBXO) family plays a key role in the malignant progression of tumors. However, the biological functions and clinical value of the FBXO family in liver cancer remain unclear. Our study comprehensively assessed the clinical value of the FBXO family in hepatocellular carcinoma (HCC) and constructed a novel signature based on the FBXO family to predict prognosis and guide precision immunotherapy.

LMNB1/CDKN1A Signaling Regulates the Cell Cycle and Promotes Hepatocellular Carcinoma Progression.

Background: Hepatocellular carcinoma (HCC) is one of the most aggressive malignancies in the world. Lamin B1 (LMNB1) is a key component of the nuclear skeleton structure. Recent studies have found that LMNB1 is overexpressed in tumor tissues and is associated with the prognosis of patients.

Structural characteristics and antioxidant activities of a novel polysaccharide from Euphorbia himalayensis root.

Euphorbia himalayensis Boiss. is an alpine member of the Euphorbiaceae family. Its dried roots have been used to treat digestive problems and chest congestion in traditional Tibetan and Mongolian medicine.

Photocatalytic Nitrogen Fixation Coupled with the Generation of Value-Added Chemicals from N and Cellulose over MoO Nanosheets.

Photocatalytic nitrogen fixation from N provides an alternative strategy for ammonia (NH) production, but it was limited by the consumption of a sacrificial electron donor for the currently reported half-reaction system. Here, we use naturally abundant and renewable cellulose as the sacrificial reagent for photocatalytic nitrogen fixation over oxygen-vacancy-modified MoO nanosheets as the photocatalyst. In this smartly designed photocatalytic system, the photooxidation of cellulose not only generates value-added chemicals but also provides electrons for the N reduction reaction and results in the production of NH with a maximum rate of 68 μmol·h·g.

Intraoperative femurofibular angle combined with tibiofibular angle measurement has fewer correction errors in open-wedge high tibial osteotomy.

Aim: This study aimed to verify the accuracy of intraoperative femurofibular angle combined with tibiofibular angle (FFA-TFA) measurement and compare it with traditional alignment line methods in open-wedge high tibial osteotomy (OWHTO).

Methods: A total of 174 knees of 122 patients undergoing OWHTO and using an alignment line or FFA-TFA measurement as an index of optimal correction were included in this retrospective study. The intraoperative alignment line passed through the targeted weight-bearing line (WBL) of the tibial plateau in the alignment line group.

Modulating band structure through introducing Cu/CuO composites for the improved visible light driven ammonia synthesis.

The photocatalytic performance of ceria-based materials can be tuned by adjusting the surface structures with decorating the transition-metal, which are considered as the important active sites. Herein, cuprous oxide-metallic copper composite-doped ceria nanorods were assembled through a simple hydrothermal reduction method. The photocatalytic ammonia synthesis rates exhibit an inverted "V-shaped" trend with increasing Cu/CuO mole ratio.

pneumonia in Wuxi, China: retrospective analysis of 55 cases and predictors of severe disease.

Purpose: More and more patients with community-acquired pneumonia have been detected with () infected using metagenomic next-generation sequencing (mNGS). Previously, this was unheard of, and several patients presented with severe pneumonia and even required ECMO. We aimed to clarify the clinical characteristics of pneumonia and find out if there are any possible predictors of severe pneumonia.

The Gomez-Lopez-Hernandez Syndrome: The Contribution of 2 Hispanic Giants of Pediatric Neurology.

The specialty of Pediatric Neurology emerged during the 20th century, a period in which many neurologists played significant roles in revolutionizing this field. Two acclaimed pediatric neurologists of Hispanic origin, Drs Manual Gomez and Arturo Lopez-Hernandez, made substantial contributions to the literature on pediatric neurology. One of their remarkable contributions was their discovery of a new, rare neurocutaneous syndrome with variable phenotype, the Gomez-Lopez-Hernandez syndrome (GLHS).

Retracted: A new aquatic beetle (Adephaga: Coptoclavidae) from the Middle Jurassic Daohugou Biota.

Tigrivia baii gen. et sp. nov.

Multicomponent hydroxides supported Cu/CuO nanoparticles for high efficient photocatalytic ammonia synthesis.

Environmentally friendly photocatalytic N fixation process has attracted considerable attention. Developing efficient photocatalysts with high electron-hole separation rates and gas adsorption capacities remains quite challenging. Herein, a facile fabrication strategy of Cu-CuO and multicomponent hydroxide S-scheme heterojunctions with carbon dot charge mediators is reported.

Electricity-Promoted Friedel-Crafts Acylation of Biarylcarboxylic Acids.

An electricity-promoted method for Friedel-Crafts acylation of biarylcarboxylic acids is described in this research. Various fluorenones can be accessed in up to 99% yields. During the acylation, electricity plays an essential role, which might motivate the chemical equilibrium by consuming the generated TFA.

3-D Time-Lapse Imaging of Cell Wall Dynamics Using Calcofluor in the Moss Physcomitrium patens.

Time-lapse imaging with fluorescence microscopy allows observation of the dynamic changes of growth and development at cellular and subcellular levels. In general, for observations over a long period, the technique requires transformation of a fluorescent protein; however, for most systems, genetic transformation is either time-consuming or technically unavailable. This manuscript presents a protocol for 3-D time-lapse imaging of cell wall dynamics over a 3 day period using calcofluor dye (which stains cellulose in the plant cell wall), developed in the moss Physcomitrium patens.

CeO-CDs clusters decorated Co(OH) nanosheets for improved photocatalytic ammonia synthesis.

The green synthesis process of photocatalytic ammonia production has received more and more attentions. Herein, a Z-scheme heterojunction with all-solid-state structures is constructed, in which carbon dots can act as electron transferring mediators. The photocatalytic measurement shows that the modified photocatalysts exhibit much higher activities, in which the ammonia production rates can reach above 232 µmol·g·h under the light irradiation.

A ROS/Akt/NF-κB Signaling Cascade Mediates Epidermal Growth Factor-Induced Epithelial-Mesenchymal Transition and Invasion in Human Breast Cancer Cells.

Background: As one of the most widely used anti-diabetic drugs for type II diabetes, metformin has been shown to exhibit anti-cancer activity in recent years. Epidermal growth factor (EGF) and its receptor, EGFR, play important roles in cancer metastasis in various tumors, including breast cancer. Epithelial-mesenchymal transition (EMT) is a critical process for cancer invasion and metastasis.

Electrochemical Synthesis of Polysubstituted Oxazoles from Ketones and Acetonitrile.

Herein we report an electrochemical strategy for the construction of polysubstituted oxazoles from easily available ketones and acetonitrile under room temperature. The method shows high efficiency, a broad substrate scope, and requires no external chemical oxidant. Mechanistic investigations suggest that the reaction proceeds through a Ritter-type reaction/oxidative cyclization using acetonitrile as the reactant and solvent.

SiP Nanosheets: A Metal-Free Two-Dimensional Photocatalyst for Visible-Light Photocatalytic H Production and Nitrogen Fixation.

Two-dimensional (2D) semiconductors for photocatalysis are more advantageous than the other photocatalytic materials since the 2D semiconductors generally have large specific surface area and abundant active sites. Phosphorus silicon (SiP), with an indirect bandgap in bulk and a direct bandgap in the monolayer, has recently emerged as an attractive 2D material because of its anisotropic layered structure, tunable bandgap, and high charge carrier mobility. However, the utilization of SiP as a photocatalyst for photocatalysis has been scarcely studied experimentally.

Physicochemical properties, immunostimulatory and antioxidant activities of a novel polysaccharide isolated from (Edgew) Heim.

Herbal medicines often contain bioactive polysaccharides. However, many medicinal herbs have not been explored for any active saccharides that may play key roles in their bioactivities. Herein, we extracted a novel polysaccharide from (Edgew) heim (denoted MHHP), a popular medicinal ingredient in traditional medicines.