Publications by authors named "Liane Ulper"

Article Synopsis
  • Semliki Forest virus (SFV) has a positive strand RNA genome that is critical for virus replication and synthesis of structural proteins through subgenomic (SG) mRNA.
  • The study evaluated 14 replication-competent vectors based on an SFV4 isolate, finding that specific elements, including certain SG promoters and IRES, were ineffective for the vectors, and that the position and length of the SG promoter influenced genetic stability.
  • A new method that generated diverse expression libraries based on these vectors successfully preserved genetic diversity and gene expression over several cell culture passages.
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In their vertebrate hosts, arboviruses such as Semliki Forest virus (SFV) (Togaviridae) generally counteract innate defenses and trigger cell death. In contrast, in mosquito cells, following an early phase of efficient virus production, a persistent infection with low levels of virus production is established. Whether arboviruses counteract RNA interference (RNAi), which provides an important antiviral defense system in mosquitoes, is an important question.

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Semliki Forest virus (SFV, genus Alphavirus) has a broad host range, high efficiency of viral protein expression, and the ability to stimulate an immune response. These properties have made SFV an attractive tool for development of expression vectors, and plasmid clones containing cDNA of the SFV genome often are used. However, instability of these plasmids resulting from cryptic expression of SFV envelope proteins in Escherichia coli represents a problem both for the development of SFV-based vectors and for SFV research.

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