Development of a New Approach for Standardization of the Herb Rafn. by High Performance Liquid Chromatography.

Objectives: The aim of this study was the development a new, fully validated high performance liquid chromatography (HPLC) method for the quantitative analysis of secoiridoid glycosides by an active marker swertiamarin in the herb Rafn. The article describes a new approach to the standardization of and more specifically the development of a new validated HPLC method for the quantitative determination of secoiridoid glycosides by swertiamarin.

Materials And Methods: The quantitative determination of swertiamarin was performed in isocratic mode on a Symmetry C18 column using water and acetonitrile as solvents for the mobile phase.