Development of a New Approach for Standardization of the Herb Rafn. by High Performance Liquid Chromatography.

Objectives: The aim of this study was the development a new, fully validated high performance liquid chromatography (HPLC) method for the quantitative analysis of secoiridoid glycosides by an active marker swertiamarin in the herb Rafn. The article describes a new approach to the standardization of and more specifically the development of a new validated HPLC method for the quantitative determination of secoiridoid glycosides by swertiamarin.

Materials And Methods: The quantitative determination of swertiamarin was performed in isocratic mode on a Symmetry C18 column using water and acetonitrile as solvents for the mobile phase.

Estimation of the influence of the environmental factors on the accumulation of phytochemicals and antioxidant capacity in the ivy leaves ( L.).

This study aimed at evaluating the impact of environmental factors on the accumulation of various components in collected from different European countries. The obtained results of studied samples showed that the antioxidant capacities were in the range of 0.027 to 0.