Phenolic Compounds from the Rhizomes of Smilax china L. and Their Anti-Inflammatory Activity.

A new triflavanoid, kandelin B-5 (), was isolated from the rhizomes of L., together with six known phenylpropanoid substituted flavan-3-ols (-), nine flavonoids (-), two stilbenoids (, ), and two other compounds (, ). The structure of compound was determined on the basis of 1D, 2D NMR and HR-ESI-MS data, as well as chemical method.

[Sodium tanshinone IIA sulfonate prevents postoperative peritoneal adhesions in rats by enhancing the activity of the peritoneal fibrinolytic system].

Objective: To evaluate the effect of sodium tanshinone IIA sulfonate (STS) in preventing postoperative peritoneal adhesions in rats and explore the mechanisms.

Methods: Sixty SD rats were randomized into 4 equal groups, including a blank control group, adhesion model group, and high-, moderate-, and low-dose STS-treated groups, and were subjected to injuries of the parietal peritoneum and cecum to induce peritoneal adhesions, followed by intraperitoneal administration of saline and STS at the doses of 20, 10, and 5 mg/kg for 7 consecutive days, respectively. Another 15 untreated rats served as the blank control group.

[Prostaglandin E2 promotes hepatocellular carcinoma cell proliferation through EP2 prostanoid receptor].

Objective: To investigate the effect of prostaglandin E2 (PGE(2)) on the proliferation of cultured hepatocellular carcinoma cells and explore which subtypes of EP prostanoid receptor mediate the action.

Methods: RT-PCR was used to determine COX-2 and EP receptor mRNA expression levels in human hepatocellular carcinoma cell line Hep3B and human normal hepatocyte line QSG7701. Cell counting kit-8 (CCK-8) assay was employed to investigate the effect of PGE(2), selective EP2 receptor agonist butaprost and EP3/EP4 receptor agonist PGE1 alcohol on the proliferation of the cells.

[Suppressive effect of matrine on cell growth and decreases beta-catenin-dependent transcriptional activity in hepatoma cell line Hep3B].

Objective: To investigate the effects of matrine on proliferation, cell cycle, apoptosis rate and beta-catenin-dependent transcriptional activity in cultured hepatoma cell line Hep3B.

Methods: Cell viability was analyzed by cell counting kit-8 (CCK-8) assay. Cell cycle and apoptosis rate wene determined by flow cytometry analysis, beta-catenin-dependent transcriptional activity was assayed with Dual-Luciferase Reporter System.

[Effects of compound Centella asiatica enema on kidneys coefficient, electrolytes and blood in chronic renal failure rats].

Objective: To study the pharmacological effects of compound Centella asiatica enema on chronic renal failure (CRF) rats.

Methods: Rats were divided into control group, CRF model group, Niaoduqing positive control group, compound Centella asiatica enema high, middle and low three groups kidney coefficient, electrolyte levels, hematocrit (HCT), red blood cell counts (RBC), hemoglobin (HGB) content were observed after 30 days's treatment.

Results: Compared with the model group, the level of the electrolyte, HCT, RBC, HGB of rats in compound Centella asiatica enema high-doses group and the Niaoduqing group were significantly improved.

[Preparation and in vitro drug release study of long-circulating hydroxycamptothecin nanoparticles].

Objective: To prepare long-circulating hydroxycamptothecin nanoparticles and study its in vitro drug release characteristics.

Methods: The HCPT-PEG-PCL-NPs were prepared by solvent-diffusion method using PEG-PCL block copolymer synthesized as a matrix and HCPT as an antitumor agent. Then the obtained NPs were evaluated and its in vitro drug release characteristics were investigated.

[Effect of Changtong oral liquid on fibroblast proliferation in normal and adhesive rat peritoneal tissues].

Objective: To investigate the effect of Changtong oral liquid (CTOL) on the proliferation of cultured fibroblasts derived from normal peritoneum (NFs) and adhesive peritoneum (AFs) of rats.

Methods: Twenty male SD rats were randomized into 4 groups, including a normal serum group and 3 CTOL groups with CTOL treatment at low, medium or high doses. Serum samples were obtained from the abdominal arteries of the rats after oral treatment with CTOL for 7 days.

[Preparation and characterization of hydroxycamptothecin nanoparticles of amphiphilic block copolymer].

Objective: To prepare hydroxycamptothecin nanoparticles of amphiphilic block copolymer and study its characterization initially.

Methods: Polyethyleneglycol-polycaprolactone (PEG-PCL) was synthesized and its structure was characterized by 1H-NMR. The HCPT-PEG-PCL-NPs were prepared by solvent-diffusion method using PEG-PCL block copolymer as a matrix and HCPT as an antitumor agent.

[Quantitative determination of 1-deoxynojirimycin in mulberry leaves by high-performance liquid chromatographic-tandem mass/mass spectrometry].

Objective: To develop a high-performance liquid chromatographic-tandem mass/mass spectrometric method to determine the concentration of 1-deoxynojirimycin (1-DNJ) in mulberry leaves.

Methods: 1-deoxynojirimycin was separated on an SHIMADZU HRC-NH2 column with the mobile phase consisting of acetonitrile and 0.1% formic acid.

Antitumor effects of polysorbate-80 coated gemcitabine polybutylcyanoacrylate nanoparticles in vitro and its pharmacodynamics in vivo on C6 glioma cells of a brain tumor model.

Polybutylcyanoacrylate (PBCA) nanoparticles coated with polysorbate-80 have been extensively studied for delivery of drugs into the animal models; however, 1% polysorbate-80 coated gemcitabine PBCA nanoparticles (GCTB-PBCA-NPs) remain unknown. In this study, we investigated the inhibitory effects of brain targeted 1% polysorbate-80 coated GCTB-PBCA-NPs on C6 glioma cells in vitro and in vivo. GCTB-PBCA-NPs were prepared by emulsion polymerization and freeze drying.

[Thyroid-induced hyperthyroidism Yin-deficiency model of rats].

Objective: To establish a rat model of hyperthyroidism Yin-deficiency.

Methods: SD rats separate male and female were randomly divided into four groups, a total of eight groups, each with six. The first group for the control group, 2 to 4 for the experimental drug-treated group, including thyroid-low, medium and high-dose group.

[Effect of shenshuaining dispersible tablets on the levels of NO, NOS, SOD and MDA in kidney of chronic renal failure rats].

Objective: To observe the effect of Shenshuaining dispersible tablets on the levels of NO, NOS, SOD and MDA in kidney of chronic renal failure (CRF) rats induced by adenine.

Methods: Seventy-two Sprague-Dawley rats were randomly divided into 6 groups: normal control group, model control group, Niaoduqing group, Shenshuaining dispersible tablets group(high, middle and low dose). From the second to the sixth group, the rats were prepared for model of CRF by being fed 0.

[Determination of arsenic content in Changtong oral liquid by inductively coupled plasma- mass spectrometry].

To determine arsenic content in Changtong oral liquid, inductively coupled plasma-mass spectrometry (ICP-MS) was employed, which generated linear calibration curves in the range of 5-25 ng/ml for As (r=0.9998). The average recovery of As was 98.

[Preparation of 10-hydroxycamptothecin semisolid lipid nanoparticles and investigation of its stability].

Objective: To prepare 10-hydroxycamptothecin-semisolid lipid nanoparticles (HCPT-SSLN) and investigate its stability.

Methods: HCPT-SSLN was prepared by the method of "emulsion evaporation at a high temperature and solidification at a low temperature"; The morphology was examined by transmission electron microscope; The particle size and xi potential were determined by laser granularity equipment; The physical stability of both suspl and freeze drying powder of HCPT-SSLN were investigated.

Results: The mean particle size of the prepared HCPT-SSLN was 130.

Effect of Changtong oral liquid on serum cytokine concentrations in rats with postoperative intestinal adhesion.

Objective: To observe the effects of Changtong oral liquid (CTOL) on the serum levels of tumor necrosis factor (TNF)-alpha, transforming growth factor (TGF)-beta(1), interleukin (IL)-1beta, IL-4, IL-6 and IL-10 in rats with postoperative intestinal adhesions.

Methods: Fifty-four male Sprague-Dawley rats were randomized into 6 equal groups, namely the normal control, model, Simo decoction (SMD) groups and three CTOL groups of low, moderate, and high doses, respectively. Intestinal adhesion was induced in the rats of the groups other than the normal control group.

Chinese medicine compound Changtong oral liquid on postoperative intestinal adhesions.

Aim: The aim of this study was to observe the effect of a Chinese medicine compound Changtong oral liquid (CT) on tissue plasminogen activity (t-PA), plasminogen activator inhibitor (PAI), TGF-beta1 and hydroxyproline (OHP).

Methods: Two sets of animal experiments were performed in the present study. Forty New Zealand rabbits and 48 Sprague-Dawley (SD) rats were assigned randomly to one of the five groups: sham adhesion, adhesion with saline, adhesion with low dosage of the CT, adhesion with middle dosage of the CT and adhesion with high dosage of the CT.

[Changtong oral liquid for prevention of postoperative intestinal adhesion: an experimental study].

Objective: To observe the effect Changtong oral liquid (CTOL), a traditional Chinese drug preparation, on the prevention of postoperative intestinal adhesion.

Methods: Fifty-four male SD rats were randomly divided into 6 equal groups, namely the normal control, model control, Simo decoction (SMD, another traditional Chinese drug preparation), and CTOL groups, and CTOL group included three subgroups treated at low, moderate and high doses. Except for those in the normal control group, all the other rats were subjected to operation with Ellis' method for establishing intestinal adhesion models.

[Determination of dexamethasone sodium phosphate content in Fuyankang cream by high-performance capillary electrophoresis].

Objective: To determine the content of dexamethasone sodium phosphate in Fuyankang cream, a preparation for treatment of various skin conditions.

Methods: Dexamethasone sodium phosphate content in Fuyankang cream was determined using high-performance capillary electrophoresis (HPCE) at the detection wavelength of 254 nm, under the optimal condition achieved with a fused-silica capillary tube (60 cm x 75 microm) and 100 mmol/L sodium tetraborate buffer (pH 9.2) at a constant voltage of 25 kV with a sampling time of 10 s at 25 degrees Celsius;.