[The Application of Estrogen Receptor Aptamer in Immunohistochemical Detection of Breast Cancer].

Objective: To synthesize and select an estrogen receptors aptamer that can be used in immunostaining of breast cancer tissues.

Methods: ER protein was purified. ER aptamer that showed a high affinity and specificity for ER was synthesized and selected and by SELEX.

Expression of TRAIL in liver tissue from patients with different outcomes of HBV infection.

Objectives: Hepatitis B virus (HBV) infection triggers the production of TRAIL, suggesting that TRAIL may play a role in liver injury after HBV infection. However, it remains unclear whether TRAIL expression in liver tissue correlates with the extent of liver injury caused by HBV infection. The aim of this article was to investigate the correlation of TRAIL expression and disease severity.

Efficacy of consensus interferon in treatment of HbeAg-positive chronic hepatitis B: a multicentre, randomized controlled trial.

Background: Consensus interferon (CIFN) is a newly developed type I interferon.

Aims: This multicentre, controlled trial was conducted to determine the efficacy of CIFN and to compare it with alpha-1b-interferon (IFN-alpha1b) in the treatment of patients with hepatitis B e antigen (HBeAg)-positive chronic hepatitis B.

Methods: 144 Patients were randomly assigned to receive 9 microg CIFN (CIFN group) or 50 microg INF-alpha1b (IFN-alpha group) subcutaneously 3 times weekly for 24 weeks, followed by 24 weeks of observation.

Clinical significance of 4 patients with chronic hepatitis B achieving HBsAg clearance by treated with pegylated interferon alpha-2a for less than 1 year: a short report.

We report 4 chinese patients with hepatitis B e antigen-positive chronic hepatitis B achieving clearance of HBsAg by using pegylated interferon alpha-2a for less than 1 year, to provide one clinical clue for the treatment of chronic hepatitis B.

Elective caesarean section versus vaginal delivery for preventing mother to child transmission of hepatitis B virus--a systematic review.

Background: Caesarean section before labor or before ruptured membranes ("elective caesarean section", or ECS) has been introduced as an intervention for preventing mother-to-child transmission (MTCT) of hepatitis B virus (HBV). Currently, no evidence that ECS versus vaginal delivery reduces the rate of MTCT of HBV has been generally provided. The aim of this review is to assess, from randomized control trails (RCTs), the efficacy and safety of ECS versus vaginal delivery in preventing mother-to-child HBV transmission.

[The study about immune response to Balb/c mice inoculated with hepatitis B virus DNA vaccine pCI/S2S].

Objective: To construct the hepatitis B virus (HBV) DNA vaccine pCI/S2S, and study the expression of specific antigen protein in muscle tissue section and the specific immune responses of humoral and cellular immunity in BALB/c mice being inoculated with pCI/S2S.

Methods: DNA templates were acquired from the mixed serum of patients with chronic hepatitis B, and the gene fragment S2S of HBV was cloned. The pCI vector and DNA S2S fragment were doubly cut by Kpn I and Not I, and then linked.

[Immunoprotection role of a hepatitis B virus DNA vaccine responsible to BAlb/c mice].

Objective: To investigate the specific immune response and the immunoprotection effects of cytotoxic T-lymphocyte (CTL), which is induced by hepatitis B virus (HBV) DNA vaccine pCMV-S2S, on BALB/c mouse with the attack of SP2/0-S2S cells, a BALB/c mouse myeloma cell line stably expressing the HBV preS2S antigen.

Methods: BALB/c mice were divided into 3 groups: experimental group (intramuscularly injected with pCMV-S2S), control group 1 (HBsAg vaccine immunizing) or control group 2 (plasmid pCMV immunizing), which were immunized thrice at week 0.4 and 8 respectively.

[On the role of liver-enriched transcription factors in regulating HBV transcription and replication].

Objective: To investigate the effects of various liver-enriched transcription factors in regulating HBV transcription and replication, and to explore their potential roles in HBV hepatotropism.

Methods: The replication-competent HBV recombinant plasmid pHBV4.1 plus different liver-enriched transcription factor (HNF1, HNF3, HNF4, HNF6, C/EBP and RXRa/PPARa) expression plasmids were co-transfected into nonhepatic cell lines (NIH3T3, HeLa, 293T, SW1353, CV-1 and COS1).

[Experimental study of liver fibrosis reversal effect of warming-yang compound formula ganzhifu].

Objective: To observe the effect of Ganzhifu (GZF), a Chinese compound formula for Warming-yang on experimental hepatic fibrosis, and to explore its mechanism.

Methods: Fifty-nine Wistar rats were divided into the normal control group and the model group. After the hepatic fibrosis model was established in rats by subcutaneous injection of CCl4 and feeding 10% alcohol in the model group, the rats were randomly sub-divided into the Ganzhifu group, colchicine group and model group, they were treated with Ganzhifu, colchicine and normal saline of same volume once a day respectively for 30 days as one treatment course.

[Immune response enhanced by genes encoding IFN-alpha 8 and T alpha 1 co-inoculated with HBV DNA vaccine].

Objectives: To evaluate if the humoral immune response of hepatitis B DNA vaccine pVAX1-S2S could be enhanced by Talpha1 and/or IFNa expression plasmid co-inoculated.

Methods: The following mammalian expression recombinant plasmids were constructed: the plasmid pVAX1-S2S expressing hepatitis B surface antigen S2S, the plasmid pVAX1-T/I co-expressing thymosin a and IFNalpha, the plasmid pVAX1-I/S2S co-expressing IFNalpha and S2S. These plasmids were inoculated intramuscularly into several BALB/c mice groups in different combinations.

[Full-length sequence of hepatitis B virus isolated from high incidence hepatocellular carcinoma area-Longan county].

Objective: To understand full-length sequence of HBV isolated from high incidence hepatocellular carcinoma area-Longan county, Guangxi.

Methods: The nested polymerase chain reaction (nPCR) was used for amplifying the whole HBV DNA in sera of asymptomatic carriers. The products were sequenced by clone sequencing and homological analysis.

Early diagnosis of bacterial and fungal infection in chronic cholestatic hepatitis B.

Aim: To investigate the early diagnostic methods of bacterial and fungal infection in patients with chronic cholestatic hepatitis B.

Methods: One hundred and one adult in-patients with chronic hepatitis B were studied and divided into 3 groups: direct bilirubin (DBil)/total bilirubin (TBil) > or = 0.5, without bacterial and fungal infection (group A, n=38); DBil/TBil <0.

[Study of infusion of oxygen-enriched liquid to correct severe hypoxemia in infectious diseases: a report of pilot clinical study].

Objective: To investigate a new therapy for effectively correcting severe hypoxemia in patients with infectious diseases by infusion of oxygen-enriched liquid, in order to raise the partial pressure of blood oxygen without passing through pathologically damaged alveoli of such patients.

Methods: Intravenous drip with oxygen-enriched liquids was given to 6 cases suffering from severe acute respiratory syndrome (SARS), and 3 cases of acquired immune deficiency syndrome (AIDS) in the course of treatment for 1 to 5 days, 500-700 ml per day.

Results: For all the 9 SARS cases, their hypoxemia was gradually corrected to normal in 20 minutes' or 4 hours' intravenous drip with oxygen-enriched liquid.

Cloning of HBsAg-encoded genes in different vectors and their expression in eukaryotic cells.

Aim: To compare the efficiency of different plasmids as DNA vectors by cloning three HBsAg-encoded genes into two eukaryotic expression vectors, pRc/CMV and pSG5UTPL/Flag, and to express HBsAg S, MS, and LS proteins in SP2/0 cells, and to establish monoclone SP2/0 cell strains that are capable of expressing S or S2S proteins stably.

Methods: Segments of S, preS2-S, preS1-preS2-S genes of Hepatitis B virus were amplified by routine PCR and preS1-S fragment was amplified by Over-Lap Extension PCR. The amplified segments were cleaved with restricted endonuclease Hind III/Not I followed by ligation with pRc/CMV, or BamHI/EcoR I followed by ligation with pSG5UTPL/Flag.

[Early indexes to predict the therapeutic effect of interferon on chronic hepatitis B].

Objective: To summarize the clinical changing characters of the clinical markers after interferon treatment in chronic hepatitis B (CHB) and make out practical indexes to predict the effect.

Methods: 150 CHB patients were randomly divided into two groups: therapeutic group (90) and control group (60) in the prospective controlled trial. The levels of endogenous interferon before treatment, interferon antibody at the end of the second month and fourth month after treatment, alanine aminotransferase (ALT) and HBV DNA in the serum were detected.