Identification of MHC I class genes in two Platyrrhini species.

The major histocompatibility complex is a diverse gene family that plays a crucial role in the adaptive immune system. In humans, the MHC class I genes consist of the classical loci of HLA-A, -B, and -C, and the nonclassical loci HLA-E, -F, and -G. In Platyrrhini species, few MHC class I genes have been described so far and were classified as MHC-E, MHC-F, and MHC-G, with MHC-G possibly representing a classical MHC class I locus while there were arguments about the existence of the MHC-B locus in Platyrrhini.

[Cloning and characterization of p43 cDNA from Trichinella spiralis].

Objective: To clone and characterize the p43 cDNA from muscle larvae cDNA library of Trichinella spiralis (Ts) Chinese isolate.

Methods: PCR technique was used to amplify the target cDNA from muscle larvae cDNA library. After cloned in pMD18T vector, it was transformed into E.

[Expression and antigenicity analysis of p46000 antigen from newborn larvae of Trichinella spiralis].

Objective: To express and analyze p46000 antigen from newborn larvae of Trichinella spiralis.

Methods: p46000 antigen gene was subcloned to the pET28a expression system by PCR. The recombinant transformant was induced by IPTG and the antigenicity was analyzed with ELISA and Western blotting.

[Immunoscreening of newborn larvae cDNA library of Trichinella spiralis].

Objective: To obtain antigenic genes encoding newborn larvae antigens of Trichinella spiralis.

Methods: Newborn larvae (NBL) cDNA library of Trichinella spiralis was screened using the infected swine serum and positive clones were sequenced and analysed.

Results: 158 positive clones were obtained from 4.