Molecular Basis for the Biosynthesis of an Unusual Chain-Fused Polyketide, Gregatin A.

Gregatin A () is a fungal polyketide featuring an alkylated furanone core, but the biosynthetic mechanism to furnish the intriguing molecular skeleton has yet to be elucidated. Herein, we have identified the biosynthetic gene cluster of gregatin A () in sp. sh18 and investigated the mechanism that produces the intriguing structure of by in vivo and in vitro reconstitution of its biosynthesis.