[Developing traditional Chinese medicine injection is the need for curing sickness to save patients].

Safety issues of traditional Chinese medicine injections has been heated debate. There are two diametrically opposed views: it should be used reasonable and developed healthily or be forbidden to use. Some people have many misunderstandings and prejudices about the safety of traditional Chinese medicine injections.

Neuroprotective effect of α-mangostin on mitochondrial dysfunction and α-synuclein aggregation in rotenone-induced model of Parkinson's disease in differentiated SH-SY5Y cells.

The study was designed to evaluate the protective effect of α-mangostin and explore its mechanism in an in vitro model of Parkinson's disease (PD) induced by rotenone. SH-SY5Y cells were treated with rotenone and α-mangostin for 24 h. α-Mangostin significantly and concentration-dependently inhibited rotenone-induced cytotoxicity.

Allicin improves cardiac function by protecting against apoptosis in rat model of myocardial infarction.

Objective: To study the effects of allicin on cardiac function and underlying mechanism in rat model of myocardial infarction (MI).

Methods: Ninety-four Wistar rats were randomly assigned to 6 groups (n=14-16 per group): sham control group [underwent thoracotomy without left anterior descending (LAD) occlusion and only received an injection of the same amount of citrate buffer], MI control group (subjected to LAD occlusion and only received an injection of same amount of citrate buffer), positive control group (subjected to LAD occlusion and received an injection of diltiazem hydrochloride at the dose of 1.5 mg/kg), and MI + allicin groups (subjected to LAD occlusion and received an injection of allicin at the doses of 1.

[Relationship between law on the protection of wildlife and animal-derived drugs].

In most countries, the protection of wildlife resources is in a negative way, the state do not put emphasis on or even oppose artificial breeding, with the poor results. In our country, the state shall pursue a policy of strengthening the protection of wildlife resources, actively domesticating and breeding the species of wildlife, which has made great achievements. Traditional Chinese medicine(TCM) is one pillar of medical and health service in China, animal-derived drugs which are effective drugs for the treatment of certain critical cases have been used in clinic for 2 000 years.

[Specificity of traditional Chinese medicine's clinical re-evaluation].

Clinical re-evaluation is to verify the drug's safety and effectiveness again,while the drug itself has not been improved. However, due to the complexity of traditional Chinese medicine, ingredients in bulk drugs, prescription, productive processes, quality standards and other aspects need to be enhanced. So improving the quality, safety and effectiveness of traditional Chinese medicine by clinical re-evaluation is also very necessary.

Effect of Erzhi Pill (二至丸) on improving cerebral nerve cell apoptosis in aging rats.

Objective: To investigate the effects of Erzhi Pill (二至丸,EZP) on nerve cell apoptosis in senescence model rats.

Methods: The rats model of senescence was established by peritoneal D-galactose injection combined with thymusectomy. Forty SD rats were randomized into four groups, the normal control group, the senescence model group, the EZP treated group, and the vitamins treated group, 10 in each group.

[Progress on antineoplastic constituents derived from polypore fungi].

Polypore fungi is a cluster of important pharmacological fungi with significant antitumor activity. In recent years, the antineoplastic constituents from polypore fungi have been comprehensively studied. Through investigating the domestic and overseas studied paper, the antitumor active constituents derived from polypore fungi including high molecular weight compounds such as polysaccharides, glycopeptides, glycoproteins, lectins, and lipid soluble low molecular weight compounds such as terpenoids, steroids, phenolics, benzopyranones, were reviewed.

[Effects of guanxin II on cardiac protein expression after acute myocardial ischemia on rats].

Objective: Guanxin II is a famous modern formula of traditional Chinese medicine. Guanxin II after myocardial infarction (MI) has been shown to have beneficial effects on cardiac anatomy and function. The purpose of this study was to examine the effects of Guanxin 1I on cardiac protein expression after MI.

[Hepatic and renal injury induced by Radix Aristolochiae or Guanxin Suhe Wan for a long-term in rats].

Objective: To evaluate the toxicity of Radix Aristolochiae supplied experimental evidence of rational use of drug in clinic.

Method: After treatment with small dose Radix Aristolochiae, Guanxin Suhe Wan (with Radix Aristolochiae) and Guanxin Suhe Wan (without Radix Aristolochiae) in different group for a long- term, respectively, the biochemical indicator of PT, ALT, AST, ALB, ALP, Crea and BUN were detected, and the kidney, liver, stomach and urinary bladder were examined by pathologic assaying.

Result: In Radix Aristolochiae group and Guanxin Suhe Wan (with Radix Aristolochiae) group, all of biochemical indicator were changed significantly, and hepatonecrosis, renal tubular necrosis, gastric carcinoma and bladder carcinoma were discovered.

Comparison of cardioprotective effects of salvianolic acid B and benazepril on large myocardial infarction in rats.

In the present study, we compared cardioprotective effects of salvianolic acid B (Sal B) and the angiotension-converting enzyme inhibitor, benazepril, in rats with large myocardial infarction (MI). The large MI was produced by coronary artery ligation for 4 weeks in rats. The rats were divided into the following groups: sham operation; MI; MI + Sal B (100 mg/kg by a gavage, once a day for 4 weeks) and MI + benazepril (1 mg/kg by a gavage, once a day for 4 weeks).

[Urgency and necessity of standardisation of chinese medicine with confusions of compound Danshen preparations].

Through analysing the complicated phenomenon of the traditional Chinese medicine product, we propose the standardization for the traditional Chinese medicine. Taking compound Danshen preparation for example, the status of our standardization of the traditional Chinese medicine is connected with the chaos of the raw material, vehicle, production process, quality criteria and clinical application. So we propose the countermeatures to strengthen the construction of standardization for the traditional Chinese medicine.

Beta-aescin: a potent natural inhibitor of proliferation and inducer of apoptosis in human chronic myeloid leukemia K562 cells in vitro.

Beta-aescin, a natural triterpenoid saponin isolated from the seed of Chinese horse chestnut (Aesculus chinensis), is known to generate a wide variety of biochemical and pharmacological effects. In the present study, the authors investigated the anti-proliferative and apoptotic effects of beta-aescin in human chronic myeloid leukemia K562 cell line in vitro. The anti-proliferative effects were detected by CFU-K562 colony formation and cell viability assay.

[Nephrotoxicity of Radix Aristolochice and it's substitution material Radix Inulae in rats].

Objective: To evaluate the toxicity of Radix Aristolochiae and Radix Inulae, and to supply the toxicity experimental data that Radix Inulae supersedes Radix Aristolochiae in clinic.

Method: A long dose of Radix Aristolochice and Radix Inulae was given intragastrically to rats for six months, then drug withdrawal for a month. The hematology and biochemical indicators were measured, and the pathologic changes of kidney, liver, stomach and urinary bladder were examined.

[Effects of series of Muskone on heart hemodynamics and myocardial consumption of oxygen in experimental dogs].

Objective: To observe the effects of series of Muskone (the muskone includes Slender Dutchmanspipe Root, Inula Root and neither kind of Common Aucklandia Root) on the heart hemodynamics and myocardial consumption of oxygen in experimental dogs, and to explain its pharmacological action on cardiovascular system.

Method: Arterial blood pressure, coronary blood flow, resistance in coronary artery, total peripheral resistance, work of left artrium and oxygen consumption index of the cardiac muscles were observed in anaesthetic dogs.

Result: The series of Muskone decreased arterial blood pressure significantly, dilated coronary artery and peripheral arteries significantly, increased coronary blood flow, decreased resistance in coronary artery, improved the work of left artrium, the oxygen availability of cardiac muscles and the complaisance of arteries in cardiac muscles.

Increased inflammatory factors activity in model rats with experimental autoimmune prostatitis.

Male rats were immunized with prostate tissue homogenate supernate (PTHS) of male rats with complete Freund's adjuvant (CFA) intra dermal in the multiple points and simultaneously immunized with 0.5 ml Pertussis-Diphtheria-Tetanus (PDT) vaccine intra peritonea on 0 and 30th day. At the 45th day after first immunization, animals were sacrificed and a series of examinations such as HE stain, assay of TNF-alpha by ELISA and assay of inducible nitric oxide synthase (iNOS) mRNA by in-situ hybridization (ISH) were taken.

[Study on therapeutic effects of series of muskone on myocardial infarction canines].

Objective: To observe the effects of the series of Muskone (the Muskone includes Slender Dutchmanspipe Root, Tumuxiang, and not Slender Dutchmanspipe Root) on myocardial ischemia, myocardial infarction and hematological index in experimental canines, and to explain the pharmacological action and characteristic of its therapeutic effect on ischemic heart disease.

Method: The range and degree of myocardial ischemia was evaluated by epicardial electrogram mapping, and the range extent of myocardial infarction was determined by quantitate histology (N-BT staining method). Meanwhile, the changes of ET, TXB2, 6-Keto-PGF1alpha were determined to study the effects of the series of Muskone on myocardial ischemia, myocardial infarction and hematological index in experimental canines.

Effects of fenofibrate and xuezhikang on high-fat diet-induced non-alcoholic fatty liver disease.

1. Fenofibrate and xuezhikang are two types of drugs widely used in the treatment of dyslipidaemia in China. The main purpose of present study was to test the efficacies and explore the potential mechanisms of action of the two lipid-lowering agents on high-fat diet-induced non-alcoholic fatty liver disease (NAFLD).

[Study on comparative pharmacology of series of Muskone].

Objective: To study the therapeutic effects of the series of Muskone (the Muskone includes Slender Dutchmanspipe Root, Tumuxiang, and not Slender Dutchmanspipe Root) on experimental myocardial infarct and pain in rats.

Method: Coronary artery ligation was applied for the model of myocardial infarct. Therapeutic effects were evaluated by measuring parameters of histomorphometry, blood plasm of ET, 6- keto-PGF1alpha and TXB2.

[Effect of vitexia-rhamnoside (V-R) on vasomotor factors expression of endothelial cell].

Objective: To observe the influence of vitexia-rhamnoside (V-R) on vasomotor factor expression of endothelial cell (EC) damaged by hypoxia and reoxygenation.

Method: The cultured human umbilical vein endothelial cells (HUV ECs) were subject to ischemia and reperfusion following hypoxia and reoxygenation. The levels of ET-1, NO and NOS intracellular in culture supertanants were measured by radioimmunity, Griess and immunohistochemistry, respectively.

[Protective effects of San Baoxin on injury induced by myocardical ischemic reperfusion and anti-thrombosis in rats].

Objective: To investigate the effects of San Baoxin on myocardial injury induced by ischemia-reperfusion (MI/R) and thrombogenesis in rats in vivo and ex vivo.

Method: The experimental model was established by ligation of left anterior descending coronary artery for 30 min followed by reperfusion for 180 min. The Chandler method was used to produced ex vivo thrombosis and an electrical stimulation of common carotid artery was adopted to form in vivo thrombosis respectively, the effect of antithrombosis induced by San Baoxin was observed.

[Experimental studies on menopausal syndrome treated by traditional Chinese medicine].

In this paper, literature about experimental researches on female climacteric syndrome (FCS) treated by traditional Chinese medicine (TCM) published in recent years were reviewed. This paper reviewed not only experimental researches about the effects of nervous system-endocrine and immune regulating net work, the vegetative nervous function and free radical treated by TCM; but also concerned the experimental approaches of animal selected, model established; and objective indexes of FCS. Taking advantages of FCS by integrative medicine is available for enhancing the clinical efficacy.

[Progress on the research of prevention and treatment of female climacteric syndrome by traditional Chinese medicine].

Perimenopause syndrome, referred also to climacteric syndrome, results from the changing of relationship among hypothalamus, pituitary and ovary during women's aging process. Those changes take place first in overy, then in hypothalmus and pituitary, which are reflected as the functional changes in endocrinological and central nervous system, accompanied with a series of psychological symptoms. 90% of women with perimenopause syndrome show clinical symptoms.

Effects of Guiyuanfang and autologous transplantation of bone marrow stem cells on rats with liver fibrosis.

Aim: To investigate the therapeutic effects of Guiyuanfang and bone marrow stem cells (BMSCs) on rats with liver fibrosis.

Methods: Liver fibrosis model was induced by carbon tetrachloride, ethanol, high lipid and assessed biochemically and histologically. Liver function and hydroxyproline contents of liver tissue were determined.

[A preliminary investigation on safety evaluation system for health foods].

Objective: In order to guarantee the safety of health foods, a safety evaluation system has been initially proposed.

Method: Based on both experienced and experimental safe data of health foods, different safety criteria could be divided.

Result: A safety evaluation system with five criteria has been finally established, ingredients listed in the first three safe criteria were suitable for health foods.