A rapid liquid chromatography-tandem mass spectrometry based method for the detection of Tet(X) resistance gene in .

There is a major public health threat posed by antibiotic resistance around the world. Tigecycline overcomes the resistance mechanisms of traditional tetracyclines and is often seen as the final resort in combating infections caused by bacteria resistant to multiple drugs. However, the introduction of new mobile tet(X) tetracycline destructases is leading to a notable rise in tigecycline resistance.

Fe/Pt-doped carbon nanoparticles with peroxidase-like activity for point-of-care determination of uric acid.

A pasting-3D microfluidic paper-based analytical device (P-3D μPAD) was developed. It enabled an efficient cascade reaction between urate oxidase (UOX) and Fe/Pt-doped carbon nanoparticles (Fe/Pt-CNPs) for visual colorimetric detection of uric acid (UA). The novel Fe/Pt-CNP nanozyme performed high peroxidase-like activity toward 3,3',5,5'-tetramethylbenzidine (TMB) and HO with Michaelis - Menten constants (K) of 0.

Association Between Neuropsychiatric Event and Systemic Lupus Erythematosus Clinical Manifestation: A Meta-Analysis.

To uncover the clinical characteristics and investigate the underlying causes of psychiatric manifestations in patients with systemic lupus erythematosus (SLE), including its subset, neuropsychiatric systemic lupus erythematosus (NPSLE). Through comprehensive database searches in PubMed, Embase, Medline, and Cochrane, from their inception to August 2023, the study focused on adult SLE and NPSLE cases. From the selected studies, data were synthesised via a random-effects meta-analysis, encompassing a total of 2,997 subjects across six studies.

A whole-animal phenotypic drug screen identifies suppressors of atherogenic lipoproteins.

Lipoproteins are essential for lipid transport in all bilaterians. A single Apolipoprotein B (ApoB) molecule is the inseparable structural scaffold of each ApoB-containing lipoprotein (B-lps), which are responsible for transporting lipids to peripheral tissues. The cellular mechanisms that regulate ApoB and B-lp production, secretion, transport, and degradation remain to be fully defined.

Engineering Nano-Pills to Inhibit Ovarian Cancer Proliferation and Migration through a Combination of Chemical/Nucleic Acid Therapy.

Ovarian cancer (OC) is the most fatal of all gynecological malignancies, presenting a significant threat to women's health. Its treatment is complicated by severe dose-dependent chemotherapy toxicity, drug resistance, and tumor migration. Herein, an intelligent combination strategy of chemotherapy and nucleic acid therapy, named ApMEmiR&D is developed.

The causal role of immune cells in primary Sjögren's syndrome: A two-sample Mendelian randomization.

Background: Primary Sjögren's syndrome (pSS) is an autoimmune disease characterized by the destruction of exocrine glands primarily via T-cell-mediated B-cell over-activation and cytokine production. This leads to pronounced dryness of the mouth and eyes and can result in multi-systemic involvement affecting the kidneys, lungs, and blood. In recent years, there has been increasing attention on the role of immune cells in pSS.

LNCRNA 1197 Promotes the Progression of Fibroblast-Like Synovial Cells by Targeting miR-206 in Rheumatoid Arthritis Patients: A Pilot Study.

Objective: We studied the role of LNCRNA 1197 in rheumatoid arthritis (RA) and its underlying mechanism.

Methods: We detected expression of LNCRNA 1197 in RA fibroblast-like synovial cells (RA-FLS) by RT-qPCR. Functional experiments were conducted to assess the impact of LNCRNA on cells as demonstrated by a Transwell assay.

Artificial intelligence for predicting treatment responses in autoimmune rheumatic diseases: advancements, challenges, and future perspectives.

Autoimmune rheumatic diseases (ARD) present a significant global health challenge characterized by a rising prevalence. These highly heterogeneous diseases involve complex pathophysiological mechanisms, leading to variable treatment efficacies across individuals. This variability underscores the need for personalized and precise treatment strategies.

Risk factors and assessment of subclinical atherosclerosis in patients with psoriatic arthritis.

Objective: To understand the prevalence of subclinical atherosclerosis (SCA) in psoriatic arthritis (PsA) patients; to explore the correlation between PsA combined with SCA and traditional cardiovascular risk factors and disease activity; to compare the role of Framingham Risk Score (FRS) and atherosclerotic cardiovascular disease (ASCVD) scores.

Methods: We included 50 PsA patients who met the CASPAR classification criteria, 50 diabetes patients and 50 healthy people. Clinical data were collected from all patients, minimal disease activity (MDA), disease activity index for psoriatic arthritis (DAPSA), ASCVD, FRS were assessed in patients with PsA, and carotid artery intima-media thickness was measured.

Hepatocyte-Targeted Lipid Nanoparticle Delivery of HERC2 Plasmid Controls Drug-Induced Hepatotoxicity by Limiting β-Catenin-Regulated CYP2E1 Expression.

Understanding the molecular mechanisms that bridge hepatic inflammation and liver injury is crucial for developing effective therapeutic strategies for drug-induced liver injury (DILI) management. HECT domain and RCC1-like domain 2 (HERC2) belongs to the large Herc family of ubiquitin E3 ligases, which are implicated in tissue development and inflammation. The observation reveals a pronounced HERC2 expression in specific hepatocyte subsets that proliferate in response to DILI in humans, prompting an investigation into the role of HERC2 in distinct DILI progression.

Induced pluripotent stem cell-derived mesenchymal stem cells: whether they can become new stars of cell therapy.

Stem cell therapy constitutes a pivotal subject in contemporary discourse, with donor stem cells having been employed in research and clinical treatments for several decades. Primary cell transplantation encompasses diverse stem cell types, including ectomesenchymal stem cells, hematopoietic stem cells, and various stem cell derivatives such as vesicles and extracellular vesicles. Nevertheless, the emergence of cell engineering techniques has heralded a new epoch in stem cell therapy, markedly broadening their therapeutic potential.

A Patient-Prioritized Research Agenda for Clinical Trials in Kidney Stone Disease.

Purpose: To ensure that research on kidney stones provides meaningful impact for the kidney stone community, patients and caregivers should be engaged as stakeholders in clinical trial design, starting at study inception. This project aimed to elicit, refine, and prioritize research ideas from kidney stone stakeholders to develop a patient-centered research agenda for clinical trials.

Materials And Methods: The Kidney Stone Engagement Core, a group of patients, caregivers, advocates, clinicians, and researchers, executed an iterative process of surveys and focus groups to elicit and refine research themes, which were then translated into research questions.

Observation of polarity changes in Sjogren's syndrome mice using a targeting lysosomes and ratiometric fluorescent probe.

Utilizing non-invasive, real-time dynamic imaging and high-resolution detection tools to track polarity changes in Sjögren's syndrome (SS) contributes to a better understanding of the disease progression. Herein, a ratiometric polarity-sensitive fluorescent probe (DIM) was designed and synthesized, DIM consisted of dicyanoisophorone as the fluorophore and morpholine moiety as lysosome targeting. DIM showed a ratiometric response to polarity and high selectivity (unaffected by viscosity, pH, ROS, RNS, etc.

Application of artificial intelligence in rheumatic disease: a bibliometric analysis.

The utilization of artificial intelligence (AI) in rheumatic diseases has enhanced the diagnostic accuracy of rheumatic diseases, enabled the prediction of patient outcomes, expanded treatment options, and facilitated the provision of individualized medical solutions. The research in this field has been progressively growing in recent years. Consequently, there is a need for bibliometric analysis to elucidate the current state of advancement and predominant research foci in AI applications within rheumatic diseases.

Role of hsa_circ_0007482 in pterygium development: insights into proliferation, apoptosis, and clinical correlations.

Aim: To investigate the impact of hsa_circ_0007482 on the proliferation and apoptosis of human pterygium fibroblasts (HPFs) and its correlation with the severity grades of pterygium.

Methods: Pterygium and normal conjunctival tissues were collected from the superior area of the same patient's eye (=33). The correlation between pterygium severity and hsa_circ_0007482 expression using quantitative reverse-transcription polymerase chain reaction (RT-qPCR) were analyzed.