Drug Treatment of Hyperlipidemia in Chinese Patients: Focus on the Use of Simvastatin and Ezetimibe Alone and in Combination.

Elevated serum low-density lipoprotein cholesterol (LDL-C) is a major risk factor for coronary heart disease (CHD). Many guidelines recommend LDL-C as a primary treatment target, and statins represent the cornerstone of treatment for lipid management. Recently revised guidelines recommend even more intense management of LDL-C, especially in patients at moderate and high risk.

Deciphering DMET genetic data: comprehensive assessment of Northwestern Han, Tibetan, Uyghur populations and their comparison to eleven 1000 genome populations.

We investigated the allele frequencies of drug absorption, distribution, metabolism and elimination (ADME)-related drug-metabolizing enzymes and transporters (DMET) genes in the Northwestern Han, Tibetan and Uyghur populations and compared the related genes in these three populations with those in eleven 1000 Genome populations. We examined 1936 single nucleotide polymorphisms of 225 DMET genes involved in ADME processes and found 732, 679 and 804 sites were polymorphic in Han, Tibetan and Uyghur. Tibetan differed from Han in only four sites (p < .

Toll-like receptor 2 and Toll-like receptor 4 exhibit distinct regulation of cancer cell stemness mediated by cell death-induced high-mobility group box 1.

Background: High-mobility group box 1 (HMGB1), a common extracellular damage associated molecular pattern molecule, is overexpressed in several solid tumors including pancreatic carcinoma. We previously observed that radiotherapy induced dying cells secrete HMGB1 and accelerate pancreatic carcinoma progression through an unclear mechanism.

Methods: Using the Millicell system as an in vitro co-culture model, we performed quantitative reverse transcriptase-polymerase chain reaction, western blot and sphere forming ability analyses to access the effect of dying-cell-derived HMGB1 on CD133 cancer cell stemness in vitro and in vivo.

Anagliptin ameliorates high glucose- induced endothelial dysfunction via suppression of NLRP3 inflammasome activation mediated by SIRT1.

High glucose- induced endothelial dysregulation has been recognized as an initiation of vascular complications in Type 2 diabetes mellitus (T2DM). Anagliptin is a novel licensed dipeptidyl peptidase-4 (DPP-4) inhibitor for the treatment of T2DM. The effects of anagliptin in high glucose- induced endothelial dysfunction are less reported.

Two ultramicroporous metal-organic frameworks assembled from binuclear secondary building units for highly selective CO/N separation.

Two novel metal-organic frameworks [Ni(μ-Cl)(BTBA)·DMF]·Cl·3DMF (JLU-MOF56, BTBA = 3,5-bis(triazol-1-yl)benzoic acid) and [Co(μ-Cl)(BTBA)·DMF]·Cl·3DMF (JLU-MOF57) have been successfully synthesized under solvothermal conditions. Crystallographic analysis indicates that the two compounds with different metal ions are isoreticular and both are constructed from binuclear [M(μ-Cl)(COO)N] (M = Co, Ni) and a 3-connected hetero-N,O donor ligand. The overall framework possesses a (3,6)-connected dag topology.

The HNF1-Regulated LncRNA HNF1-AS1 Is Involved in the Regulation of Cytochrome P450 Expression in Human Liver Tissues and Huh7 Cells.

Expression of cytochrome P450s (P450s) is regulated by epigenetic factors, such as DNA methylation, histone modifications, and noncoding RNAs through different mechanisms. Among these factors, long noncoding RNAs (lncRNAs) have been shown to play important roles in the regulation of gene expression; however, little is known about the effects of lncRNAs on the regulation of P450 expression. The aim of this study was to explore the role of lncRNAs in the regulation of P450 expression by using human liver tissues and hepatoma Huh7 cells.

Revealing transcription factor and histone modification co-localization and dynamics across cell lines by integrating ChIP-seq and RNA-seq data.

Background: Interactions among transcription factors (TFs) and histone modifications (HMs) play an important role in the precise regulation of gene expression. The context specificity of those interactions and further its dynamics in normal and disease remains largely unknown. Recent development in genomics technology enables transcription profiling by RNA-seq and protein's binding profiling by ChIP-seq.

Fungal pathogens pose a potential threat to animal and plant health in desertified and pika-burrowed alpine meadows on the Tibetan Plateau.

Intact Tibetan meadows provide significant defense against soil-borne pathogen dispersal. However, dramatic meadow degradation has been observed due to climate change and pika damage, but their impacts on soil-borne pathogens are still unclear. With approximately 40% of the world's population living in Tibetan Plateau and its downstream watersheds, this lack of knowledge should be of great concern.

Development of a Novel Gene Expression System for Secretory Production of Heterologous Proteins via the General Secretory (Sec) Pathway in .

Background: () is a potential host for the secretory production of the heterologous proteins. However, to this date few secretion-type gene expression systems in have been developed, which limit applications of in a secretory production of the heterologous proteins.

Objectives: In this study, a novel and efficient general secretory (Sec) pathway-dependent type gene expression system for the production of heterologous proteins was developed in .

Hyaluronic Acid-Functionalized Gadolinium Doped Iron Oxide Nanoparticles for Atherosclerosis-Targeted Mr Imaging.

Magnetic resonance imaging (MRI) contrast agents (CTs) with both positive () and negative (₂) contrast abilities have attracted considerable interests due to their complementary diagnostic information for more precise diagnosis. In this study, gadolinium-doped oxide nanoparticles functionalized by hyaluronic acid (HA-GdIO NPs) were synthesized for atherosclerosis-targeting -₂ dual-model MR imaging. The results showed that the HA-GdIO NPs exhibited high magnetic susceptibility and good biocompatibility.

Highly Selective Separation of Actinides from Lanthanides by Dithiophosphinic Acids: An in-Depth Investigation on Extraction, Complexation, and DFT Calculations.

To further reveal the extraction model for selective separation of trivalent actinides over lanthanides by dithiophosphinic acids (DPAHs), five representative DPAH ligands with different substituent groups have been synthesized, and their extraction and complexation behaviors toward Am/Eu have been investigated both experimentally and theoretically. The introduction of electron-withdrawing group -CF into DPAH ligands is beneficial to their extractability among the five ligands. Slope analyses show that both Am and Eu were extracted as tetra-associated species with DPAH ligands.

The competing endogenous circular RNA ADAMTS14 suppressed hepatocellular carcinoma progression through regulating microRNA-572/regulator of calcineurin 1.

Emerging evidence have discovered that circular RNAs (circRNAs) may serve as diagnostic or tumor promising biomarkers. This study aimed to investigate how circular RNA ADAMTS14 (circADAMTS14) regulates microRNA-572/ regulator of calcineurin 1(miR-572/ RCAN1) in hepatocellular carcinoma (HCC). The expression profiles of circRNA/microRNA (mRNA) between HCC tissues and paired adjacent tissues were analyzed via microarray analysis.

Metabolic engineering of for agmatine production.

Agmatine is a kind of important biogenic amine. The chemical synthesis route is not a desirable choice for industrial production of agmatine. To date, there are no reports on the fermentative production of agmatine by microorganism.

LncRNA CRNDE promotes hepatocellular carcinoma cell proliferation, invasion, and migration through regulating miR-203/ BCAT1 axis.

Objective: To investigate the impact of long noncodingRNA (lncRNA) colorectal neoplasia differentially expressed (CRNDE) on hepatocellular cancer (HCC) cell propagation, invasion, and migration by mediating miR-203/ BCAT1 axis.

Methods: Microarray analysis was based on 25 pairs of HCC cancerous tissues and adjacent tissues. The expression levels of CRNDE, miR-203, and BCAT1 in HCC tissues were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR).

Bioinformatics analysis of microarray profiling identifies the mechanism of focal adhesion kinase signalling pathway in proliferation and apoptosis of breast cancer cells modulated by green tea polyphenol epigallocatechin 3-gallate.

Objectives: This study aimed to investigate potential gene and signal pathway associated with tumour progression.

Methods: Related microarray data set of breast cancer was obtained from Gene Expression Omnibus database, and differential-expressed genes (DEGs) between two control samples and two treated samples were analysed using statistical software R. We collected 50 epigallocatechin-3-gallate(EGCG)-related genes and 119 breast cancer-related genes to create a knowledge base for following pathway analysis.

Tuning the gate opening pressure of a flexible doubly interpenetrated metal-organic framework through ligand functionalization.

A functional doubly interpenetrated MOF [(ZnO)(MDCPB)DMF]·3DMF·3HO (JLU-Liu33F, HMDCPB = 5-methyl-1,3-di(4-carboxyphenyl)benzene, DMF = N,N-dimethylformamide) with pcu topology has been solvothermally synthesized. JLU-Liu33F (F = functionalization) is the isostructural analogue of JLU-Liu33, which is constructed by -CH functional V-shaped ligand and a classical ZnO cluster. Similar to the adsorption property of JLU-Liu33, JLU-Liu33F also exhibits breathing behavior upon N and CO adsorption at 77 and 195 K, respectively.

Oral Administration of Improves the Effect of Taxane on Preventing the Progression of Breast Cancer and Reduces Its Side Effects.

We aimed to explore the efficacy and safety of (PVL) combined with taxane for treatment of patients with breast cancer (BC). The main ingredients of PVL were analyzed by high-performance liquid chromatography (HPLC). In the experiment, 424 patients with BC were evenly assigned into two groups: experimental group (EG, oral administration of PVL and taxane) and control group (CG, oral administration of placebo and taxane).

Resveratrol inhibits proliferation, migration and invasion of multiple myeloma cells via NEAT1-mediated Wnt/β-catenin signaling pathway.

Purpose: To study the influence of resveratrol on proliferation, migration and invasion of multiple myeloma (MM) cells through the long noncoding RNA (lncRNA) NEAT1 (nuclear enriched abundant transcript 1)-modulated Wnt/β-catenin signaling pathway.

Methods: Microarray analysis was used to screen aberrantly expressed lncRNAs from MM cells and normal plasmocytes. The effects of NEAT1 overexpression/silencing or resveratrol treatment on MM cell (U266 and LP1) proliferation, migration and invasion were investigated using CCK-8 (Cell Counting Kit-8), wound healing and Transwell invasion assays.

Bioactive heterocycles containing a 3,4,5-trimethoxyphenyl fragment exerting potent antiproliferative activity through microtubule destabilization.

Novel bioactive heterocycles containing a 3,4,5-trimethoxyphenyl fragment as antiproliferative agents by targeting tubulin were synthesized and their preliminary structure activity relationships (SARs) were explored. Among all these chemical agents, 2-(Benzo[d]oxazol-2-ylthio)-N-(4-methoxybenzyl)-N-(3,4,5-trimethoxyphenyl)acetamide (4d) exhibited the potent antiproliferative activity against MGC-803 cells with an IC value of 0.45 μM by induction of G2/M pahse arrest and cell apoptosis.

In situ fabrication of radiopaque microcapsules for oral delivery and real-time gastrointestinal tracking of .

Introduction: Although oral administration of is a promising approach for diseases, lack of resistance to harsh conditions and real-time tracking in gastrointestinal system in vivo are still major challenges in basic research and clinical applications.

Materials And Methods: In this study, we fabricated a chitosan-coated alginate microcapsule loaded with in situ synthesized barium sulfate (CA/BaSO microcapsule) for oral delivery and real-time X-ray computed tomography (CT) imaging. CA/BaSO microcapsules containing the were prepared in situ by one-step electrostatic spraying method, and then coated with chitosan.

Genetic polymorphisms of CYP2S1, CYP2J2 and CYP2R1 genes in three Chinese populations: Han, Tibetan and Uighur.

Aim: Cytochrome P450 enzymes CYP2S1, CYP2J2 and CYP2R1 are of interest due to their unknown enzymatic function and disease-specific expression property. The aim of this study was to investigate the genetic variations of CYP2S1, CYP2J2 and CYP2R1 genes, and their polymorphic distribution in different Chinese populations.

Materials & Methods: All of the exons, exon-intron boundaries and 1 kb 5'-flanking region of the three genes were sequenced in 150 Chinese subjects.

Single-tube multiplex real-time PCR assay for rapid and reliable detection of HLA-A*31:01 allele.

Aim: HLA-A*31:01 has been associated with carbamazepine-induced hypersensitivity reactions. HLA-A*31:01 genetic testing is recommended before the initiation of carbamazepine therapy.

Methods: A novel real-time PCR assay was designed for HLA-A*31:01 detection by allele-specific primers and TaqMan minor groove binding probes.

Surface Functionalization of Polymeric Nanoparticles with Umbilical Cord-Derived Mesenchymal Stem Cell Membrane for Tumor-Targeted Therapy.

Multiple cell plasma membranes have been utilized for surface functionalization of synthetic nanomaterials and construction of biomimetic drug delivery systems for cancer treatment. The natural characters and facile isolation of original cells facilitate the biomedical applications of plasma membranes in functionalizing nanocarriers. Human umbilical cord-derived mesenchymal stem cells (MSCs) have been identified to show tropism toward malignant lesions and have great advantages in ease of acquisition, low immunogenicity, and high proliferative ability.

MiR-29a-5p inhibits proliferation and invasion and induces apoptosis in endometrial carcinoma via targeting TPX2.

Unlabelled: This study was aimed to explore the effects of miR-29a-5p expression and its target gene TPX2 (target protein for Xenopus kinesin-like protein 2) on endometrial cancer (EC) devel on EC development and to assess the prognostic impacts of TPX2. Microarray-based GEO and TCGA (the Cancer Genome Atlas) EC expression data were used to identify differentially expressed miRNAs and mRNAs. The observed potential target relationship between miR-29a-5p and TPX2 was verified using TargetScan and luciferase reporter assays.