Automated measurement and correlation analysis of fundus tessellation and optic disc characteristics in myopia.

This study aims to quantify fundus tessellated (FT) density and optic disc (OD) morphology using deep learning (DL) techniques and to investigate the correlations between these fundus characteristics and refractive function in young patients with myopia. We constructed two DL-based segmentation models to delineate the FT, OD, peripapillary atrophy (PPA), and macula at a pixel-level resolution. The study sought to identify differences in fundus characteristics between eyes categorized as having high myopia versus mild or moderate myopia.

Halophytic succulence is a driver of the leaf non-structural carbohydrate contents in plants in the arid and hyper-arid deserts of northwestern China.

Background And Aims: Non-structural carbohydrates (NSC), primarily sugars and starch, play a crucial role in plant metabolic processes and a plant's ability to tolerate and recover from drought stress. Despite their importance, our understanding of NSC characteristics in the leaves of plants that thrive in hyper-arid and saline environments remains limited.

Methods: To investigate the variations in leaf NSC across different species and spatial scales, and to explore their possible causes, we collected 488 leaf samples from 49 native plant species at 115 sites in the desert area of northwestern China.

Desertification indirectly affects soil fauna by reducing complexity of soil habitats and diversity of energy sources.

Soil fauna is closely linked to ecological functions such as biogeochemical cycling, soil structure, ecosystem sustainability and trophic interactions. However, little consideration has been given to how desertification influences the abundance and diversity of soil fauna in arid areas. In this study, soil fauna was sampled in four desert habitats (gravel, sand, salt and mud desert) in northwest China.

Syntax-Guided Content-Adaptive Transform for Image Compression.

The surge in image data has significantly increased the pressure on storage and transmission, posing new challenges for image compression technology. The structural texture of an image implies its statistical characteristics, which is effective for image encoding and decoding. Consequently, content-adaptive compression methods based on learning can better capture the content attributes of images, thereby enhancing encoding performance.

Fucoidan alleviates the inhibition of protein digestion by chitosan and its oligosaccharides.

Chitosan (CTS) and chitosan oligosaccharides (COS) have been widely applied in food industry due to their bioactivities and functions. However, CTS and COS with positive charges could interact with proteins, such as whey protein isolate (WPI), influencing their digestion. Interaction among CTS/COS, FUC, and WPI/enzymes was studied by spectroscopy, chromatography, and chemical methods in order to reveal the role of FUC in relieving the inhibition of protein digestibility by CTS/COS and demonstrate the action mechanisms.

Heterogeneity of leaf stoichiometry of different life forms along environmental transects in typical ecologically fragile areas of China.

The coupling between carbon (C):nitrogen (N):phosphorus (P) stoichiometry in plant leaves is closely related to ecological functions such as photosynthesis, growth, and biogeochemical cycling. To explore the biogeographic patterns, nutrient limitations, and the relationships between leaf and soil stoichiometry, as well as the factors influencing leaf stoichiometry, we quantified community-level leaf C:N:P stoichiometry in trees, shrubs, and herbs along transects with a total length of about 4300 km. The leaf C:N:P ratios of trees, shrubs, and herbs were approximately 349:13:1, 267:14:1, and 226:12:1, respectively.

Chitosan and its oligosaccharide accelerate colonic motility and reverse serum metabolites in rats after excessive protein consumption.

Excessive protein consumption (EPC) could increase the gastrointestinal burden and impair gut motility. The present study was designed to explore the improvement of chitosan (CTS) and chitosan oligosaccharide (COS) on colonic motility and serum metabolites in rats after EPC. The results of in vivo experiments fully proved that CTS and COS could improve gut motility and reverse the serum metabolites in rats as indicated by LC-MS/MS analysis, and the COS group even showed a better effect than the CTS group.

Structural characterization of a fucoidan from Ascophyllum nodosum and comparison of its protective effect against cellular oxidative stress with its analogues.

In the present study, a fucoidan fraction (ANP-3) was isolated from Ascophyllum nodosum, and the combined application of desulfation, methylation, HPGPC, HPLC-MS, FT-IR, GC-MS, NMR, and Congo red test elucidated ANP-3 (124.5 kDa) as a triple-helical sulfated polysaccharide constituted by →2)-α-Fucp3S-(1→, →3)-α-Fucp2S4S-(1→, →3,6)-β-Galp4S-(1→, →3,6)-β-Manp4S-(1→, →3,6)-β-Galp4S-(1→，→6)-β-Manp-(1→, →3)-β-Galp-(1→, α-Fucp-(1→, and α-GlcAp-(1→ residues. To better understand the relationship between the fucoidan structure of A.

Soil extracellular enzyme stoichiometry reflects microbial metabolic limitations in different desert types of northwestern China.

Soil extracellular enzyme activity (EEA) stoichiometry reflects the dynamic balance between microorganism metabolic demands for resources and nutrient availability. However, variations in metabolic limitations and their driving factors in arid desert areas with oligotrophic environments remain poorly understood. In this study, we investigated sites in different desert types in western China and measured the activities of two C-acquiring enzymes (β-1,4-glucosidase and β-D-cellobiohydrolase), two N-acquiring enzymes (β-1,4-N-acetylglucosaminidase and L-leucine aminopeptidase), and one organic-P-acquiring enzyme (alkaline phosphatase) to quantify and compare the metabolic limitations of soil microorganisms based on their EEA stoichiometry.

polysaccharide regulates gut microbiota metabolites to protect against colonic inflammation in mice.

polysaccharide (ANP) can protect against colonic inflammation but the underlying mechanism is still unclear. This study has determined the metabolites of gut microbiota regulated by ANP to reveal the mechanism of the anti-inflammation effect of ANP. Using an colonic fermentation model, the results indicate that gut microbiota could utilize a proportion of ANP to increase the concentrations of short-chain fatty acids (SCFAs) and decrease ammonia content.

Interaction between Bacteroidetes species in the fermentation of Lycium barbarum arabinogalactan.

The present study investigated the utilization of an arabinogalactan from Lycium barbarum (LBP-3) by intestinal Bacteroidetes species. The mixed-culture assay showed 58.4 % LBP-3 was utilized, and Bacteroides caccae and Phocaeicola vulgatus utilized more LBP-3 in single-culture compared to others.

HO-TiO photocatalytic degradation of chondroitin sulfate and in vivo absorption and excertion of its product.

Chondroitin sulfate (CS) is widely known for its various biological activities which are closely related to the sulfate substitution and the molecular weight. Effective degradation methods without striping sulfate groups are in a need. In the present study, a photocatalytic degradation method using HO and TiO has been developed and it could decrease the average molecular weight of CS into 5 kDa within 6 h.

Theoretical study on the electronic properties of different types of the donor:acceptor complexes.

Non-fullerene organic solar cells can be classified into four forms in line with the different types of donor (D) and acceptor (A) in the active layer: all-polymer (P:P), polymer D:small-molecule A (P:M), small-molecule D:polymer A (M:P), and all-small-molecule (M:M). On the basis of having studied the electronic properties of a large number of related monomer molecules and D:A complexes, this work constructed four groups of D:A molecular pairs as described above as examples to investigate their electronic properties with first-principles density functional theory. The results show that the absolute value of the average binding energy of the P:Pcomplex D18:P(NDI2HD-T) is larger than others, indicating the structure is relatively more stable.

Chitosan and chitosan oligosaccharide influence digestibility of whey protein isolate through electrostatic interaction.

Chitosan (CTS)/chitosan oligosaccharide (COS) and whey protein isolate (WPI) have been frequently used as food supplements, but notably, the interaction between the carbohydrate and the protein may affect the digestibility of protein. Thus, the present study focused on effects of the interaction between CTS/COS and WPI on the protein digestibility. A series of chemical and spectroscopic techniques including gel electrophoresis, gel permeation chromatography, Fourier transform-infrared (FT-IR) spectroscopy, intrinsic fluorescence (IF) spectroscopy, and circular dichroism (CD) spectroscopy were applied.

Two Fucoidans with Different Molecular Weights Inhibit Inflammation via Blocking of TLR/NF-κB Signaling Pathway Discriminately.

The present study aimed to clarify the potential mechanism of fucoidans found in on anti-inflammation and to further explore the relationship between their structures and anti-inflammation. Two novel fucoidans named ANP-6 and ANP-7 and found in , were separated and purified and their structures were elucidated by HPGPC, HPLC, GC-MS, FT-IR, NMR, and by the Congo red test. They both possessed a backbone constructed of →2)-α-L-Fuc4-(1→, →3)-α-L-Fuc24-(1→, →6)-β-D-Gal-(1→, and →3,6)-β-D-Gal4-(1→ with branches of →2)-α-L-Fuc4-(1→ and →3)-β-D-Gal-(1→.

Digestion behavior of a polysaccharide from Cyclina sinensis: An explanation for the discrepancy in its immunostimulatory activities in vitro and in vivo.

Although numerous polysaccharides have demonstrated potential immunostimulatory activities in in vitro models, only a few of them successfully stimulate the immune system in vivo. In order to explore the possible reasons for the activity loss of polysaccharides in in vivo models, the immunostimulatory activities in vitro and in vivo and the digestion behavior of a polysaccharide from Cyclina sinensis (CSP) were investigated in the present study. CSP showed obvious immunostimulatory activity in a RAW 264.

Anti-obesity effects of fucoidan in high-fat diet-fed mice vary with the gut microbiota structure.

Previous studies have indicated that fucoidan could resist high-fat-diet (HFD)-induced obesity by modulating the composition of gut microbiota. However, the outcome of dietary intervention may differ between individuals due to large inter-individual variability in gut microbiota. Thus, the present study aimed to investigate the possible discrepancy of the anti-obesity effects of fucoidan supplementation in HFD-fed mice models with different gut microbiota communities.

Differences and spatial variations of plant leaf calorific value in deserts of the Hexi Corridor, Northwestern China.

The leaf calorific value (LCV) is an important trait that indicates how efficiently a plant utilizes natural resources to capture energy. However, little is known about the LCV characteristics of plants in arid and hyper-arid environments. To investigate the spatial patterns and variations in LCV of desert plants and their possible causes, we collected 343 leaf samples of 52 species along a 1000-km transect in the desert area of northwestern China.

Preparation of Low-Molecular-Weight Fucoidan with Anticoagulant Activity by Photocatalytic Degradation Method.

It is a challenge to degrade sulfated polysaccharides without stripping sulfate groups. In the present study, a photocatalytic method was applied to degrade fucoidan, a sulfated polysaccharide from brown algae. The degradation with varying addition amounts of HO and TiO were monitored by high performance gel permeation chromatography (HPGPC) and thin layer chromatography (TLC), and fucoidan was efficiently degraded with 5% TiO and 0.

Evaluation of an OCT-AI-Based Telemedicine Platform for Retinal Disease Screening and Referral in a Primary Care Setting.

Purpose: To evaluate the performance of a telemedicine platform integrated with optical coherence tomography (OCT) and artificial intelligence (AI) techniques for retinal disease screening and referral.

Methods: We constructed an OCT-AI-based telemedicine platform and deployed it at four primary care stations located in Jing'an district, Shanghai, to detect retinal disease cases among aged groups and refer them to Shanghai Tenth People's Hospital (TENTH Hospital). Two ophthalmologists jointly graded the data set collected from this pilot application, and then the performance of this platform was analyzed from multiple aspects.

Image enhancement of color fundus photographs for age-related macular degeneration: the Shanghai Changfeng Study.

Aim: To develop and evaluate a new fundus image optimization software based on red, green, blue channels (RGB) for the evaluation of age-related macular degeneration (AMD) in the Chinese population.

Methods: Fundus images that were diagnosed as AMD from the Shanghai Changfeng Study database were analyzed to develop a standardized optimization procedure. Image brightness, contrast, and color balance were measured.

Grazing exclusion had greater effects than nitrogen addition on soil and plant community in a desert steppe, Northwest of China.

Background: The impacts of increasing nitrogen (N) deposition and overgrazing on terrestrial ecosystems have been continuously hot issues. Grazing exclusion, aimed at restoration of grassland ecosystem function and service, has been extensively applied, and considered a rapid and effective vegetation restoration method. However, the synthetic effects of exclosure and N deposition on plant and community characteristics have rarely been studied.

Structural characterization and SARS-CoV-2 inhibitory activity of a sulfated polysaccharide from Caulerpa lentillifera.

Caulerpa lentillifera (Bryopsidophyceae, Chlorophyta) is an edible seaweed attracting great attention for its expansion of farming scale and increasing consumption in these years. In the present study, a sulfated polysaccharide (CLSP-2) was isolated and separated from C. lentillifera, and its chemical structure was elucidated by a series of chemical and spectroscopic methods.