A maverick: Environmentally relevant concentrations of nonylphenol attenuate the plasmid-mediated conjugative transfer of antibiotic resistance genes.

Given that many organic pollutants have been reported to facilitate the plasmid-mediated conjugative transfer of antibiotic resistance genes (ARGs), it was naturally deduced that nonylphenol (NP) can also have this kind of effect. Whereas, this study demonstrates an entirely different result that environmentally relevant concentrations of NP attenuate plasmid-mediated ARGs conjugative transfer (maximum inhibition rate 64 %), further study show that NP exposure had no significant effect on bacterial growth, cell vitality, oxidative stress response, and expression of conjugation-relevant genes, which were reported to closely relate to the conjugative transfer in numerous studies. Conclusively, it was found that the dispersant function of NP impeded the occurrence of cell mating, thus was responsible for the decline of conjugative transfer.

Agricultural land-use change and rotation system exert considerable influences on the soil antibiotic resistome in Lake Tai Basin.

In this study, we use high-throughput quantitative polymerase chain reaction approaches to comprehensively assess the effects of agricultural land-use change on the antibiotic resistome of agricultural runoffs after rainfalls in Lake Tai Basin. For the first time in this region, our findings show that orchard runoffs harbored more diverse and abundant antibiotic resistance genes (ARGs) than traditional cropland runoffs. Network analysis demonstrated that orchard runoffs possessed a strong ability for ARG dissemination via horizontal gene transfer.

In situ characterization of change in superficial organic components of thermoacidophilic archaeon Acidianus manzaensis YN-25.

For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS, CuFeS, S, FeSO). The atomic force microscopy (AFM) morphology scanning showed that the strain acclimated with different energy substrates varied a lot in EPS amount. XANES results showed clear associations between the energy substrates and the changes in organic composition in terms of typical function groups (CO, CO and CN).

[Clinical study on negative pressure closed drainage combined with vancomycin loaded calcium sulfate and autogenous bone in the treatment of chronic osteomyelitis].

Objective: To study the clinical effects of negative pressure closed drainage combined with vancomycin loaded calcium sulfate and autogenous bone in the treatment of chronic osteomyelitis.

Methods: From June 2013 to December 2016, there were 35 cases of chronic osteomyelitis patients in our department, including 23 males and 12 females, ranging in age from 11 to 65 years old, with an average of 34 years old. The course of disease ranged from 8 to 46 months, with an average of 26 months.

Complete Genome Sequence of the Extremely Thermoacidophilic Archaeon YN-25.

The complete genome of YN-25 consists of a chromosome of 2,687,463 bp, with a G+C content of 30.62% and 2,746 coding DNA sequences. This archaeon contains a series of specific genes involved in the oxidation of elemental sulfur and reduced inorganic sulfur compounds.

Fabrication of Ag-Nanorods/Polyimide Nanocomposites and Their Thermal, Mechanical, Electrical, and Dielectric Properties.

Silver nanorods/polyimide (Ag-NRs/PI) nanocomposites with high conductivity (An order of magnitude higher than pure PI), frequency-independent dielectric permittivity (3.8-4.2) and low dielectric loss (<0.

[Quantitative detection of hydroxyl radical generated in quartz powder/phosphate buffer solution system by fluorescence spectrophotometry].

Quartz powder would release radical hydroxyl in phosphate buffer solution. In order to detect the quantity of radical hydroxyl with a quite low concentration, the present paper established a fluorescence method. According to the relationship between the concentration of 2-hydroxyl of terephthalic acid (HOTP) and fluorescence intensity at the wavelength of EX(max)/EM(max) = 316 nm/422 nm, a working standard curve was constructed.

[Effect of burn blister fluid on human mesenchymal stem cell (MSC) in vitro and its phenotypic modulation in culture].

Objective: To study the effect of blister fluid obtained from burn patient on human MSCs in vitro and its phenotypic modulation in culture.

Methods: Blister fluid from burn patients was collected at 12, 24, 48 post burn hour (PBH). The human MSCs were isolated, cultured, amplified and identified in vitro, then were divided into A (culture with 20% blister fluid collected at 12 PBH) , B (culture with 20% blister fluid collected at 24 PBH), C (culture with 20% blister fluid collected at 48 PBH), N (with ordinary culture medium) groups.

[Distribution and drug resistance analysis of bacteria in different wound infections].

Objective: To analyze the distribution and drug resistance of bacteria in different wound infections and provide evidence for wound infection control in subtropical regions.

Methods: This study involved 265 patients from 4 different departments of our hospital who experienced wound infections between July, 2007 and July, 2008. The bacterial strain distribution in the wounds and drug resistance of the bacteria were analyzed.