Publications by authors named "Li-she Zhou"

Article Synopsis
  • The study focused on using fluorescence sequencing typing technology to identify the ingredients in Liuwei Dihuang pill, a type of Chinese patent medicine.
  • The researchers amplified and sequenced DNA from the plant Paeonia suffruticosa using specially designed primers, analyzing the results with GeneMarker software.
  • The psbA-trnH fluorescence labeling primer proved effective, successfully identifying three key medicinal plants in the Liuwei Dihuang pill, highlighting the reliability of this method for distinguishing raw materials in traditional Chinese medicine.
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Several lines of evidence support the notion that MUC1 is often aberrantly expressed in gastric cancer, and it is a ligand for Helicobacter pylori. Genetic variation in MUC1 gene may confer susceptibility to H. pylori infection and gastric cancer.

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  • Researchers developed a method to distinguish Astragali Radix from Hedysari Radix using PCR to amplify specific DNA alleles from 30 and 28 samples, respectively.
  • The study involved extracting DNA, amplifying specific sequences (trnL-trnF), and optimizing PCR conditions; results showed distinct band sizes for each plant – 136 bp for Astragali Radix and 323 bp for Hedysari Radix.
  • This technique effectively identifies and authenticates these plant materials, allowing for detection of 10% Hedysari Radix DNA mixed in with Astragali Radix.
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  • The study aims to differentiate Gelsemium elegans from Lonicera japonica and related species through specific PCR amplification.
  • Thirteen G. elegans samples and 58 samples from various Lonicera species were collected, and DNA was extracted and amplified using PCR techniques.
  • The results showed that PCR effectively amplified a specific 97 bp DNA fragment for G. elegans, while no bands were produced for Lonicera samples, confirming the method's efficacy as a molecular marker for identification.
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Lian Qiao Bai Du Wan was used to study the identification of Chinese patent medicine by molecular marker technique. DNA was extracted through modified CTAB method. The psbA-trnH and rbcL sequences were gradient amplified, and PCR products were ligated with the pEASY-T5 vector and then transformed into Trans1-T1 cells, respectively.

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Article Synopsis
  • The study aimed to find a novel method for identifying Astragali Radix by analyzing its DNA sequence, specifically the ITS region.
  • Thirteen samples of Astragali Radix and six samples of various adulterants were tested using PCR to amplify the ITS sequence, followed by sequencing and comparative analysis through phylogenetic methods.
  • The results showed that the ITS sequence can reliably differentiate Astragali Radix from its adulterants, proving it to be an effective molecular marker for their identification.
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Objective: To investigate the polymorphism distribution of vitamin D receptor (VDR) gene Fok I in Mongolian population of China.

Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to analyze three genotypes FF, Ff and ff in the start codon of VDR gene (Fok I) in unrelated normal healthy Mongolian individuals of China.

Results: In the population, we obtained the allelic frequencies of 57% and 43% for (F) and (f) allele and the percentage of genotypes FF, Ff and ff to be 31%, 52%, and 17% respectively.

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