Precise magnetic resonance imaging-guided sonodynamic therapy for drug-resistant bacterial deep infection.

The precise treatment of drug-resistant deep bacterial infections remains a huge challenge in clinic. Herein, a polymer-peptide-porphyrin conjugate (PPPC), which can be real-time monitored in infectious site, is developed for accurate and deep sonodynamic therapy (SDT) based on "in vivo self-assembly" strategy. The PPPC contains four moieties, i.

Precise Detection of Gene Mutations in Fine-Needle Aspiration Specimens of the Papillary Thyroid Microcarcinoma Using Next-Generation Sequencing.

Purpose: To assess the feasibility of next-generation sequencing (NGS) to detect mutations in BRAF, RAS, TERT promoter, and TP53 genes in ultrasound-guided fine-needle aspiration (FNA) biopsy samples of the papillary thyroid microcarcinoma (PTMC).

Methods: A total of 135 FNA samples out of 135 patients with suspected PTMC were submitted for mutation testing using NGS. NGS was successfully performed in 114 specimens, while the remaining 21 samples were excluded due to insufficient amount/poor quality of DNA and sequencing failure.

Comprehensive evaluation of medullary thyroid carcinoma before surgery.

Background: Medullary thyroid carcinoma (MTC) is a rare disease, but it exhibits more aggressive behaviors. The aim of this study was to improve the diagnostic accuracy of MTC before surgery by analyzing the clinical and ultrasonic data of patients with MTC.

Methods: The study included 71 patients (96 lesions) with histopathologically proven MTC between April 2011 and September 2016 in the Cancer Hospital of Chinese Academy of Medical Sciences and Peking Union Medical College.

[Effects of Ca on nitric oxide-induced adventitious rooting in cucumber under drought stress].

Cucumber (Cucumis sativus L. 'Xinchun 4') was used to explore the relationship between nitric oxide (NO) and calcium (Ca) during adventitious rooting under drought stress. Rooting parameters, endogenous Ca fluorescent intensity and the antioxidant enzymes activity (SOD, CAT and APX) in cucumber explants under drought stress were investigated.

Factors Influencing the Reliability of Thyroid Fine-Needle Aspiration: Analysis of Thyroid Nodule Size, Guidance Mode for Aspiration and Preparation Method.

Objective: We aimed to clarify the influence of ThinPrep preparation, nodule size and guidance mode on the accuracy of thyroid fine-needle aspiration (FNA).

Methods: A total of 1,240 thyroid FNAs were reviewed and 489 cases with histological correlations were enrolled in this study.

Results: Of the 489 total cases examined, 101 were processed with both ThinPrep and conventional preparation and 388 entirely with ThinPrep.

[Sentinel lymph node biopsy in papillary thyroid cancer].

Objective: To investigate the reliability and feasibility of sentinel lymph node biopsy (SLNB) of papillary thyroid carcinoma using combination of lymphoscintigraphy, the gamma probe and methylene blue staining techniques.

Methods: Twenty-three consecutive patients with thyroid papillary carcinoma were entered in the study between August 2007 and August 2009. All cases were without clinical evidence of cervical lymph node involvement.

Hypermethylation of multiple tumor-related genes associated with DNMT3b up-regulation served as a biomarker for early diagnosis of esophageal squamous cell carcinoma.

This study was designed to determine the significance of DNA methyltransferases (DNMTs) in DNA hypermethylation in esophageal squamous cell carcinoma (ESCC) and to identify DNA methylation markers in serum for the early diagnosis of ESCC. A promoter methylation profile of 12 tumor-related genes was assessed using methylation-specific PCR in ESCC and paired non-tumor tissue samples from 47 patients. Expression levels of DNMTs were examined by real-time reverse transcription-PCR and immunohistochemistry.

[Sonographic evaluation of the special thyroid carcinoma].

Objective: To analyze the cases of thyroid carcinoma with special sonographic features, so as to increase the accuracy of sonographic diagnosis for thyroid carcinoma.

Methods: The sonographic characteristics of 9 patients with special sonographic appearance were analyzed. According to the sonographic features, they were divided into two kinds: 8 cases suffered from thyroid diffuse malignant lesions, and one case showed cystic lesion sticking out of the thyroid.

[Sonographically guided percutaneous ethanol injection for therapy of thyroid benign lesion].

Objective: To develop the microtrauma therapy for thyroid benign lesion, to evaluate efficacy of sonographically guided percutaneous ethanol injection for therapy of thyroid benign lesion.

Methods: Thirty-two patients with thyroid benign lesion were treated in Cancer Hospital, the patients had thyroid disease with cystic or cystic and solid nodule without heart and lung disease, and no allergy to ethanol, but the most diameter was below 3 cm for the solid disease. These patients were divided into two groups, the first group were 14 patients (from June 2002 to August 2003), the second group was 18 patients (from September 2003 to March 2006).

[Value of high-resolution ultrasonography in differential diagnosis of cervical cystic lesions].

Objective: To analyze ultrasonographic features of the different kinds of cervical cystic lesions, and propose differential diagnostic standard for these lesions based on the evidence of high resolution ultrasonography.

Methods: The clinical data of 107 patients with different kinds of cervical cystic lesions were analyzed. The role of gray scale and colour Doppler sonography for such lesions was prospectively studied.

[Diagnostic value of ultrasonography in thyroid lesions].

Objective: To analyse ultrasonographic mapping of the thyroid lesions, so as to summarize ultrasonographic characteristics, and improve the accuracy rate of preoperative diagnosis for thyroid lesions.

Methods: The clinical data were analyzed for 1700 patients with different thyroid lesions who were treated between January 2002 and December 2005. The appearance of gray scale and colour Doppler sonography for the lesions was prospectively studied, the different blood flow index was determined.