Assessing the impact of lime on chromium migration in soil caused by basic chromium sulfate in tannery.

Chromium (Cr) pollution is the primary pollution problem of the soil in tannery. However, the effect of tanning chemicals on Cr migration in soil has not been clearly elucidated. Column leaching tests were designed in this study to reveal the transport and transformation of Cr from basic chromium sulfate (BCS) into soil and the effects of lime on Cr migration and transformation.

Migration of leather tannins and chromium in soils under the effect of simulated rain.

Chromium (Cr) and tannin are two major pollutants in leather industry. However, little is known about the co-migration of leather tannins and Cr in soils. In this study, column experiments were conducted to estimate Cr leaching behavior from topsoil and the environmental risk of the leachate at various tannin dosages and different ways (tannin either directly adding to the Cr-contaminated soil or adding stepwise through simulated rain) into the soil.

p53 and P-glycoprotein influence chemoresistance in hepatocellular carcinoma.

Chemoresistance is a critical obstacle to the treatment of hepatocellular carcinoma (HCC). The mechanisms underlying resistance to doxorubicin, cisplatin, and 5-fluorouracil involve p53 and P-glycoprotein (P-gp). p53 plays a role in cell growth; therefore, resistance mechanisms involve chemotherapy-induced apoptosis and p53 mutation and inactivation.

LncRNA-TUSC7/miR-224 affected chemotherapy resistance of esophageal squamous cell carcinoma by competitively regulating DESC1.

Background: This study aims to clarify the underlying mechanism for the tumor suppressive function of lnc TUSC7 in chemotherapy resistance of esophageal squamous cell carcinoma (ESCC).

Methods: TUSC7, miR-224 and DESC1 expressions in ESCC tissues and cells were detected by qRT-PCR. Protein level of DESC1, EGFR and p-AKT were observed by Western blot.

Novel Mutations in SERPINF1 Result in Rare Osteogenesis Imperfecta Type VI.

Osteogenesis imperfecta (OI) is a group of inherited disorders characterized by recurrent fragile fractures. Serpin peptidase inhibitor, clade F, member 1 (SERPINF1) is known to cause a distinct, extremely rare autosomal recessive form of type VI OI. Here we report, for the first time, the detection of SERPINF1 mutations in Chinese OI patients.

Novel mutations in FKBP10 in Chinese patients with osteogenesis imperfecta and their treatment with zoledronic acid.

Osteogenesis imperfecta (OI) is a group of hereditary disorders characterized by decreased bone mass and increased fracture risk. The majority of OI cases have an autosomal dominant pattern of inheritance and are usually caused by mutations in genes encoding type I collagen. OI cases of autosomal recessive inheritance are rare, and OI type XI is attributable to mutation of the FKBP10 gene.

A cryptic balanced translocation involving COL1A2 gene disruption cause a rare type of osteogenesis imperfecta.

Background: Osteogenesis imperfecta (OI) is a group of hereditary disorders characterized by low bone mass and recurrent fractures. Most OI cases follow an autosomal dominant pattern of inheritance and are attributed to mutations in genes encoding type I collagen (COL1A1/COL1A2). Genomic structural variations involving type I collagen genes are extremely rare in OI.

Two novel mutations in TMEM38B result in rare autosomal recessive osteogenesis imperfecta.

Osteogenesis imperfecta (OI) is a group of clinically and genetically heterogeneous disorders characterized by decreased bone mass and recurrent bone fractures. Transmembrane protein 38B (TMEM38B) gene encodes trimeric intracellular cation channel type B (TRIC-B), mutations of which will lead to the rare form of autosomal recessive OI. Here we detected pathogenic gene mutations in TMEM38B and investigated its phenotypes in three children with OI from two non-consanguineous families of Chinese Han origin.

Role of microRNAs in metastasis of non-small cell lung cancer.

Lung cancer causes the most number of deaths from cancer. Non-small cell lung cancer (NSCLC), including squamous cell carcinoma, large cell carcinoma, and adenocarcinoma, is responsible for more than 80% of primary lung cancer cases. As metastasis is the main cause of death, understanding the mechanisms underlying NSCLC metastasis are essential for improving the treatment of this disease.

PU.1 Is Identified as a Novel Metastasis Suppressor in Hepatocellular Carcinoma Regulating the miR-615-5p/IGF2 Axis.

Invasion and metastasis is the major cause of tumor recurrence, difficulty for cure and low survival rate. Excavating key transcription factors, which can regulate tumor invasion and metastasis, are crucial to the development of therapeutic strategies for cancers. PU.

The design and recombinant protein expression of a consensus porcine interferon: CoPoIFN-α.

CoPoIFN-α is a recombinant non-naturally occurring porcine interferon-α (IFN-α). It was designed by scanning 17 porcine IFN-α nonallelic subtypes and assigning the most frequently occurring amino acid in each position. We used a porcine IFN-α (PoIFN-α) derived from domestic pig as a control.

The metastasis of hepatocarcinoma can be inhibited by T lymphocytes reconstitution in nude mice model.

Background/aims: The metastasis of hepatic carcinoma is correlated with the body's immune status. T lymphocytes play a big part role in tumor immune. The aim of the present study is to investigate the inhibition effects of metastatsis in nude mice bearing hepatic carcinoma after T lymphocytes reconstitution.

Enhancement of waste activated sludge aerobic digestion by electrochemical pre-treatment.

Electrochemical technology with a pair of RuO(2)/Ti mesh plate electrode is first applied to pre-treat Waste Activated Sludge (WAS) prior to aerobic digestion in this study. The effects of various operating conditions were investigated including electrolysis time, electric power, current density, initial pH of sludge and sludge concentration. The study showed that the sludge reduction increased with the electrolysis time, electric power or current density, while decreased with the sludge concentration.

[The experimental results of GVHD following orthotropic liver transplantation].

Objective: To analyze experimental results of Graft-versus-host disease (GVHD) after liver transplantation.

Methods: 13 cases of GVHD out of the 1013 liver transplantation between 2002-2008 were analysed. Routine blood test, liver function and microorganisms test were done in all of the 13 cases, bone marrow test was done in 5 cases, liver pathological test was done in 5 cases, cytokines were analyzed in 4 cases, chimerism test was done in 6 cases.

[Analysis of 643 colorectal cancer patients diagnosed by total colonoscopy].

Objective: To evaluate the value of colonoscopy in the diagnosis of colorectal cancer.

Methods: The data of colonoscopy in 1751 patients from May 2003 to June 2006 were retrospectively analyzed.

Results: Of these 1751 colonoscopies, 643 colorectal cancers (36.

[Functional localization of metastasis suppressor genes for HCC on human chromosome 8].

Objective: We previously showed that introduction of a normal, neomycin-tagged human chromosome 8 reduced the metastatic capacity of C5F rat liver cancer cell line, which had high metastatic potential without affecting tumorigenicity, suggesting the presence of one or more metastasis suppressor genes encoded on human chromosome 8. We proceeded to define further the region harboring the metastasis suppressor gene(s) and to determine the random loss of human chromosome 8 by PCR amplification of sequence tag site (STS) markers.

Methods: The national Center for Biotechnology Information (NCBI) databases were used as references of the relative genetic distances of the STS markers.

[Relation between changes of dendritic cell function and down-regulation of beta-centractin in hepatocellular carcinoma].

Objective: To investigate the relation between the changes of dendritic cell (DC) function and down-regulation of beta-centractin in hepatocellular carcinoma.

Methods: DC derived from peripheral blood were cultured and then pulsed by lysates from hepatocarcinoma cells (HCC) with high, low, or none metastatic potential of the lines HCCLM6, MHCC97L, and Hep3B, and from normal human liver cell of the line Chang liver. DC not pulsed were used as control group.

[A preliminary study of the killing function in vitro by T lymphocytes activated by dendritic cells loaded with exosomes secreted by hepatic cancer cell lines with high or low metastatic potentials].

Objective: To study the tumor cell killing function of T lymphocytes stimulated by dendritic cells (DC) and to analyze the differences of protein contents of exosomes in each type of cell.

Methods: The exosomes of hepatic cell lines with high (P group) or low (F group) metastatic potentials were isolated by a process of four-step centrifugation and the collected exosomes were observed under an electron microscope (EM). The tumor cell killing experiment was performed by adding T lymphocytes activated by DC loaded with exosomes from corresponding P and F group cells and was studied using 3H-TdR experiments.

[The relationship between lymphangiogenesis and lymphatic metastasis in murine hepatic carcinoma of high and low metastatic potentialities].

Objectives: To study the relationship between lymphangiogenesis and lymphatic metastasis in mice bearing hepatic carcinoma and analyze the mechanism of the lymphatic metastasis.

Methods: Hepatic carcinoma cell lines of high and low potentialities of lymphatic metastasis were injected into the footpads of Balb/c mice. Their metastases to lymph nodes were examined.

[Influence of reconstruction of immunological functions of T lymphocytes on mouse hepatocarcinoma metastasis].

Objective: To investigate the effectiveness of reconstruction of immunological functions of T cells on the degree of metastases of mouse hepatocarcinoma and the mechanisms of their functioning.

Methods: The T cell model of immunological functions in Balb/c nu/nu mice was established and the effectiveness of the model was evaluated. The mice were divided into 4 groups.

[Relationship between DLC-1 expressions and metastasis in hepatocellular carcinoma].

Objectives: To study the relationship between the expression level of DLC-1 mRNA (located in 8p) and the invasion/metastasis of human hepatocellular carcinoma (HCC).

Methods: Fifty-one surgical specimens of human HCC were divided into high-invasive and low invasive groups according to their clinicopathological features. DLC-1 mRNA expression was studied in the 51 HCC specimens as well as 5 different metastasis potential cell lines using real-time quantitative PCR (RQ-PCR).