Publications by authors named "Li-hua Wan"

Following the publication of the above article, a concerned reader drew to the authors' attention that, among Figs. 1D, 2A and 4B, certain of the control western blots had been re‑used in different blots. The authors have retrieved and re‑examined their original data, and were able to identify the correct control western blots where the data had been inadvertently duplicated in the affected original figures.

View Article and Find Full Text PDF

Background: Plants live with diverse microbial communities which profoundly affect multiple facets of host performance, but if and how host development impacts the assembly, functions and microbial interactions of crop microbiomes are poorly understood. Here we examined both bacterial and fungal communities across soils, epiphytic and endophytic niches of leaf and root, and plastic leaf of fake plant (representing environment-originating microbes) at three developmental stages of maize at two contrasting sites, and further explored the potential function of phylloplane microbiomes based on metagenomics.

Results: Our results suggested that plant developmental stage had a much stronger influence on the microbial diversity, composition and interkingdom networks in plant compartments than in soils, with the strongest effect in the phylloplane.

View Article and Find Full Text PDF

The characteristic life stages of infesting blowflies (Calliphoridae) such as Chrysomya megacephala (Fabricius) are powerful evidence for estimating the death time of a corpse, but an established reference of developmental times for local blowfly species is required. We determined the developmental rates of C. megacephala from southwest China at seven constant temperatures (16-34°C).

View Article and Find Full Text PDF

Examining the succession pattern of carrion insects on vertebrate carcasses is widely accepted as an effective method to estimate the postmortem interval (PMI) of decayed bodies. Investigation of the community of sarcosaprophagous insects, especially flies and beetles, is the foundation of the succession pattern study. This study aimed to investigate the sarcosaprophagous beetles succession on animal carcasses in the suburban area of southwestern China and to establish a basic catalog for forensic application.

View Article and Find Full Text PDF

In this study, simultaneous nitrification and denitrification (SND) coupled Phosphorus removal process through gradually decreasing DO concentration was investigated by treating wastewater with a low COD/TN ratio (C/N = 3 : 1-4: 1) in a sequencing batch reactor (SBR) inoculated with aerobic granular sludge (AGS). Successful SND coupled Phosphorus phenomenon occurred after 20d at the DO concentration of 0.50-1.

View Article and Find Full Text PDF

Blowflies (Calliphoridae) are recognized as a powerful tool for estimating the minimum postmortem interval (PMImin). The times for blowflies to develop from oviposition to eclosion is mainly controlled by temperature, which can differ between even closely related species. Hemipyrellia ligurriens (Wiedemann) (Diptera: Calliphoridae) is a blowfly distributed throughout Asia and Australia.

View Article and Find Full Text PDF
Article Synopsis
  • This study explored the connection between the expression of the survivin gene, an apoptotic inhibitor, and prognosis in nasopharyngeal carcinoma patients.
  • Survivin protein and mRNA levels were significantly higher in nasopharyngeal carcinoma samples (79.5% and 75%, respectively) compared to chronic nasopharyngitis, suggesting its potential role in cancer progression.
  • The study concluded that higher survivin expression correlates with shorter survival times, indicating its importance for both understanding disease development and improving prognosis.
View Article and Find Full Text PDF

Colon cancer is one of the most common malignancies, causes considerable morbidity and mortality. The current treatment for colon cancer is more modest than had been hoped. There is an urgent clinical need to explore new agents or adjuvants for colon cancer treatment.

View Article and Find Full Text PDF

Objective: To observe the effect of diets with different fat levels on the body size and development of Lucilia sericata.

Methods: Under the constant temperature of 28 degrees C, the larvae were reared on the diets containing 0% (G0), 10% (G1), 30% (G3), 50% (G5) and 80% (G8) fat tissues (fat/muscle ratio), respectively. Length and weight of larvae and pupae were measured at 12 h interval since 16 h after eclosion.

View Article and Find Full Text PDF

Objective: To observe the effect of feeding on different pig tissues on the development of Lucilia sericata larvae.

Methods: Under a constant temperature of 25 degrees C, about 200 larvae each were reared on four different substrates, i.e.

View Article and Find Full Text PDF

Inferring the ancestral origin of DNA samples can be helpful in correcting population stratification in disease association studies or guiding crime investigations. Populations throughout the world vary in appearance features and biological characteristics. Based on this idea, we performed a genome-wide scan for SNPs within genes that are related to physical and biological traits.

View Article and Find Full Text PDF

Objective: To observe the effect of feeding on different pig tissues on the development of Chrysomya megacephala larvae.

Methods: About 200 larvae each were reared on four different substrates, i.e.

View Article and Find Full Text PDF

Objective: To investigate the genetic polymorphisms of 11 Y-chromosomal short tandem repeats (STR) loci in Chongqing Tujia population, and to evaluate their forensic application values and genetic relationships with the other 16 populations of China.

Methods: Eleven Y-STR loci in 215 unrelated Tujia individuals from Chongqing were amplified with PowerPlex Y System, and the PCR products were analyzed by 310 Genetic Analyzer. Cluster analysis and phylogenic trees were applied to show the genetic distance among the populations.

View Article and Find Full Text PDF

Objective: To observe the length heteroplasmy and point heteroplasmy in human mtDNA control region.

Methods: The peripheral blood, buccal cell, and single hair shaft from 50 individuals and 16 family members, related in their maternallineage were analyzed by direct sequencing, and clones from 20 individuals whose mtDNA sequences have a T-C transition at 16189 nt were sequenced.

Results: No point heteroplasmy were observed in peripheral blood, buccal cell, single hair shaft from the same individual, neither in maternally related individuals.

View Article and Find Full Text PDF