mRBPome capture identifies the RNA-binding protein TRIM71, an essential regulator of spermatogonial differentiation.

Continual spermatogenesis relies on the actions of an undifferentiated spermatogonial population that is composed of stem cells and progenitors. Here, using mouse models, we explored the role of RNA-binding proteins (RBPs) in regulation of the biological activities of this population. Proteins bound to polyadenylated RNAs in primary cultures of undifferentiated spermatogonia were captured with oligo (dT)-conjugated beads after UV-crosslinking and profiled by proteomics (termed mRBPome capture), yielding a putative repertoire of 473 RBPs.

Cideb controls sterol-regulated ER export of SREBP/SCAP by promoting cargo loading at ER exit sites.

SREBPs are master regulators of lipid homeostasis and undergo sterol-regulated export from ER to Golgi apparatus for processing and activation via COPII-coated vesicles. While COPII recognizes SREBP through its escort protein SCAP, factor(s) specifically promoting SREBP/SCAP loading to the COPII machinery remains unknown. Here, we show that the ER/lipid droplet-associated protein Cideb selectively promotes the loading of SREBP/SCAP into COPII vesicles.

[Development and Output Characteristics of 785 nm Portable Grating-Coupled External Cavity Tunable Semiconductor Laser].

Aiming at the miniaturization requirement of shifted excitation Raman spectroscopy test system, a portable grating-coupled external cavity (EC) tunable semiconductor laser in Littrow configuration is designed and fabricated with a commercial 785 nm high-power laser diode as the gain device. By using a new wavelength tuning method, aiming to change the position of gain device relative to the collimating lens in the horizontal direction, a miniaturized device with the size of 140 mm×65 mm×50 mm is designed. Compared to the traditional wavelength tuning method which is to change the light incident angle by rotating the diffraction grating, this new tuning method reduces the translational distance of semiconductor gain device effectively, thus it is conductive to the fast and broad wavelength tuning of portable EC laser.