POU2F2-mediated upregulation of lncRNA inhibits ferroptosis in breast cancer via miR-376c-3p/SLC7A11 axis.

Triple-negative breast cancer (TNBC) is a subtype of BC with high rates of mortality. The mechanism of in ferroptosis of TNBC was investigated. Chromatin immunoprecipitation and dual-luciferase reporter assays were used to measure intermolecular relationships.

Circulating miR-221 expression level and prognosis of cutaneous malignant melanoma.

Background: The aim of this study was to investigate the feasibility of using serum miR-221 as a noninvasive prognostic biomarker for cutaneous malignant melanoma (CMM).

Material/methods: We measured the expression levels of miR-221 in serum samples from 72 CMM patients and 54 healthy controls by real-time quantitative polymerase chain reaction (RT-PCR). The overall survival (OS) and disease-free survival (DFS) were calculated using the Kaplan-Meier method.

Rapid and sensitive detection of Cronobacter spp. (previously Enterobacter sakazakii) in food by duplex PCR combined with capillary electrophoresis-laser-induced fluorescence detector.

Cronobacter spp. (Enterobacter sakazakii) is an emerging opportunistic pathogen with a 40-80% mortality rate in infants and immunocompromised crowd resulting from the consumption of contaminated food. A novel method for detecting Cronobacter spp.

Multivariate analysis of molecular indicators for postoperative liver metastasis in colorectal cancer cases.

Aims: To explore the relationship between various molecular makers and liver metastasis of colorectal cancer (CRC).

Method: Using immunohistochemistry, protein expression of CEA, nm23, c-met, MMP2, COX- 2, VEGF, EGFR, and CD44 was assessed in 80 CRC cases. The Chi-square test and logistic regression were performed to analyze the relationship between these indicators and CRC liver metastasis.

[Determination of dimethyl sulphate in environmental samples with GC-ECD].

Objective: To develop an gas chromatography-electrophoretic capture detection (GC-ECD)method for determining dimethyl sulphate (DMS) in environmental samples.

Methods: The environmental samples were dissolved with acetone and the DMS was reacted with KI. The reaction product of methyl iodide was extracted with Hexane and determined by GC-ECD, with HP-5 (30.

[Determination of biphenyl ether herbicides in water using HPLC with cloud-point extraction].

Objective: To determine residues of multiple biphenyl ether herbicides simultaneously in water using high performance liquid chromatography (HPLC) with cloud-point extraction.

Methods: The residues of eight biphenyl ether herbicides (including bentazone, fomesafen, acifluorfen, aclonifen, bifenox, fluoroglycofenethy, nitrofen, oxyfluorfen) in water samples were extracted with cloud-point extraction of Triton X-114. The analytes were separated and determined using reverse phase HPLC with ultraviolet detector at 300 nm.

[Determination of adenosine in food by high performance anion exchange chromatography].

Objective: To establish a high performance anion chromatography method for the determination of adenosine in food.

Methods: Adenosine in the food sample was extracted with Acetonitrile-water, and separated with Amino PAC PA10 Amino acid-separation column, with 0.25 mol/L NaOH as the mobile phase.

[Detecting nicotine and its metabolite cotinine in human hair with capillary gas chromatography].

Objective: To develop a rapid method for determination of nicotine and its metabolite cotinine in human hair with capillary gas chromatography with flame ionization detector.

Methods: The hair sample was digested by 1.5 mol/L sodium hydroxide solution.

[Simultaneous determination of monosaccharides and oligosaccharides in Lycium barbarum L. by high performance liquid chromatography].

Objective: To develop a rapid approach of refractive index detection with high performance liquid chromatography (HPLC) for the determination of rhamnose, fructose, glucose, surose and maltose in Lycium barbarum L.

Methods: The sample was extracted with water and the analyte was separated by Zorbax Carbohydrate column with acetonitrile-water as mobile phase.

Results: Good linear correlations between the concentrations and the peak areas of the analyte were found, with the correlation coefficients ranging from 0.

[Detecting VA, VD3, VE and beta-carotene with HPLC in human serums].

Objective: To develop a method of detecting retinol (vitamin A), vitamin D3, alpha-tocopherol (vitamin E) and beta-carotene in human serums with HPLC.

Methods: Proteins were precipitated with anhydrous ethanol. Fat-solutable vitamins in human serums were extracted with aether/petroleum ether mixture and determined with HPLC.

[Simultaneous determination of iron, copper and cobalt in food samples by diode array detection-flow injection analysis using partial least squares calibration model].

A flow injection-CCD-diode array detection spectrophotometric method using partial least squares (PLS) algorithm for the simultaneous determination of iron, copper and cobalt in food samples has been established. The method was based on the chromogenic reaction between metallic ions and 5-Br-PADAP in the presence of acetic acid-acetic sodium buffer solution (pH 5) containing 30 g x L(-1) ascorbic acid and 2% (phi) Triton X-100. The overlapped spectra of these complexes were collected by CCD diode array detector and the multi-wavelength absorbance data were processed using partial least squares algorithm.

[Comparative study on long-term results of laparoscopic and open radical resection for colorectal carcinoma].

Objective: To compare the long-term results of laparoscopic and open radical resection for colorectal carcinoma.

Methods: Two hundred and fifteen patients with colorectal cancer from January 1996 to September 2000 were non-randomly divided into laparoscopic and open operation groups. Local recurrence, distant metastasis, 5-year survival rate and long-term postoperative complications were compared between the two groups.

[Determination of mannitol in health food by capillary gas chromatography].

Objective: To develop a capillary gas chromatographic method for the determination of mannitol in health food.

Methods: Mannitol was extracted from the sample with water by ultrasonic wave. The aliquot of sample solution was acetylated to form mannitol hexacetate with lower boiling point.

[CCD-array detection-flow injection analysis of oligomeric: proanthocyanidins in health food].

A flow-injection-CCD array detection spectrophotometry for the determination of oligomeric proanthocyanidin (OPC) in health foods was developed in the present paper, based on the reaction that OPC can form red anthocyanidin in hydrochloric acid medium with ferric ion as a catalytic agent. The absorption spectrum of OPC was scanned and the absorbance at 545 nm was determined by flow injection with CCD-array detector. The influencing factors of the chromogenic reaction and parameters of the flow injection analysis were optimized.

[Rapid analysis of genetically modified soybean by a duplex PCR-capillary electrophoresis system with laser-induced fluorescence detection].

Objective: To develop a rapid detection method for genetically modified soybean resistant to glyphosate.

Methods: A duplex PCR was performed with primers designed in this study to simultaneously amplify heterogenous genes in transgenic soybean: CaMV-35S promoter, NOS terminator and CP4-EPSPS gene. And a simple capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) was developed and applied to the rapid analysis of the above PCR products by using a 50 cm length x 100 microm i.

[Determination of 4-nonylphenol and bisphenol A in rat serum by high performance liquid chromatography with fluorescence detection].

Objective: This study was directed to the development of a simple and highly sensitive method for determination of 4-nonylphenol (4-NP) and bisphenol A (BPA) in rat serum using HPLC with fluorescence detector.

Methods: 4-NP and BPA in serum samples were extracted by a mixed solvent of n-hexane and diethyl ether (70:30), the organic layer was dried with nitrogen flow and dissolved with mobile phase. The operating conditions such as C18 column (250 mm x 4.

[Study on a sensitive setup of capillary electrophoresis with laser induced fluorescence detector and its application].

Objective: To develop a setup of capillary electrophoresis with laser induced fluorescence detector for separating and detecting DNA fragments and protein.

Methods: The setup is assembled of He-Ne Laser with 543.5 nm as the excitation light, filter, monochromator, photomultiplier tube and a computer for controlling the system and collecting the electropherograms.

[Simultaneous determination of copper and iron by flow-injection catalytic kinetic spectrophotometry with multi-channel detector].

The simultaneous determination of copper and iron by catalytic kinetic spectrophotometric method with back propagating artifical neural net (BP-ANN) was studied based on the catalysis of Cu(II) and Fe(III) to the reaction of rhodamine B hydrogen peroxide in the range of pH 3-9. The determination was performed by the construction of pH gradient and a CCD-diode array detector with a microcomputer. The optimum conditions of reaction and flow injection were studied.