Identification of watermelon H3K4 and H3K27 genes and their expression profiles during watermelon fruit development.

Background: The ClaH3K4s and ClaH3K27s gene families are subfamilies of the SET family, each with a highly conserved SET structure domain and a PHD structural domain. Both participate in histone protein methylation, which affects the chromosome structure and gene expression, and is essential for fruit growth and development.

Methods And Results: In order to demonstrate the structure and expression characteristics of ClaH3K4s and ClaH3K27s in watermelon, members of the watermelon H3K4 and H3K27 gene families were identified, and their chromosomal localization, gene structure, and protein structural domains were analyzed.

Lineage-specific amplification and epigenetic regulation of LTR-retrotransposons contribute to the structure, evolution, and function of Fabaceae species.

Background: Long terminal repeat (LTR)-retrotransposons (LTR-RTs) are ubiquitous and make up the majority of nearly all sequenced plant genomes, whereas their pivotal roles in genome evolution, gene expression regulation as well as their epigenetic regulation are still not well understood, especially in a large number of closely related species.

Results: Here, we analyzed the abundance and dynamic evolution of LTR-RTs in 54 species from an economically and agronomically important family, Fabaceae, and also selected two representative species for further analysis in expression of associated genes, transcriptional activity and DNA methylation patterns of LTR-RTs. Annotation results revealed highly varied proportions of LTR-RTs in these genomes (5.

[Comparative Analysis of Primary and Reactivated EB Virus Infection Associated Hemophagocytic Lymphohistiocytosis].

Objective: To compare the clinical characteristics of children with hemophagocytic lymphocytosis (HLH) associated with primary Epstein-Barr virus (EBV) infection and EBV reactivation, and explore the effects of different EBV infection status on the clinical indexes and prognosis of HLH.

Methods: The clinical data of 51 children with EBV associated HLH treated in Henan Children's Hospital from June 2016 to June 2021 were collected. According to the detection results of plasma EBV antibody spectrum, they were divided into EBV primary infection-associated HLH group (18 cases) and EBV reactivation-associated HLH group (33 cases).

An Open-Label Exploratory Clinical Trial Evaluating the Effects of GLS (Coptidis Rhizoma-Evodiae Fructus 2 : 1) on Fibroblast Growth Factor 21 in Patients with Nonalcoholic Fatty Liver Disease.

Methods: In a 12-week, open-label, exploratory clinical trial, 126 NAFLD patients were randomly divided into the GLS group (lifestyle intervention plus GLS) or the polyene phosphatidylcholine (PPC) group (lifestyle intervention plus PPC). Random numbers generated by DPS software were used in combination with opaque, sealed envelopes for allocation concealment. At baseline as well as at the end of the study, anthropometric parameters, glucose, lipids, hepatic enzymes, and FGF 21 were measured, with hepatic fat accumulation assessed by ultrasound (US) and US-based controlled attenuation parameter (CAP).

Sulfonated polydivinylbenzene bamboo-like nanotube stabilized Pickering emulsion for effective oxidation of olefins to 1,2-diol.

Sulfonated polydivinylbenzene bamboo-like nanotube (SPDVB) with effective olefins oxidation activity is prepared by combining cationic polymerization and sulfonation. By merely adjusting sulfonation time, SPDVB with different sulfonic acid group (-SOH) contents is achieved. SPDVB is used as both a solid emulsifier and catalyst to fabricate Pickering emulsion interface catalytic system for oxidizing olefins with 30% HO acting as oxidant/water phase and olefins acting as reactants/oil phase.

[Expression Levels of HES1, C-MYC and NF-kB in Peripheral Blood of Patients with T Cell Acute Lymphoblastic Leukemia and Their Significance].

Objective: To analyze and investigate the expression levels of HES1, C-MYC and NF-kB in peripheral blood of patients with T cell acute lymphoblastic leukemia (T-ALL) and their significance.

Methods: Sixty patients with T-ALL and 60 patients with acute myelogenous leukemia (AML) diagnosed in our hospital from June 2012 to March 2015 were enrolled in T-ALL group and AML group, respectively. Another 30 healthy people were enrolled in the control group.

[Clinicopathological Analysis of Children's Systemic EBV-Positive T-Cell Lymphoma].

Objective: To explore the clinical and pathologic features as well as prognosis of systemic EBV-positive T-cell lymphoma in children.

Methods: The clinical data including clinical manifestation, pathologic changes and treatment in 16 patients with children's systemic EBV-positive T-cell lymphoma were analyzed retrospectively, and follow-up of patients were carried out.

Results: The 16 cases included 12 males and 4  females with median age of 3.

Quantitative efficacy of three antipsychotic drugs for schizophrenia based on a real-world study in China.

Atypical antipsychotics exert remarkable long-term efficacy on the personal and social functions of schizophrenic patients. However, quantitative information on the social function of schizophrenic patients treated with atypical antipsychotics is scarce in the current clinical guidelines. In this study, we established pharmacodynamic models to quantify the time-efficacy relationship of three antipsychotic drugs based on the data from a real-world study conducted in China.

Effective solvent-free oxidation of cyclohexene to allylic products with oxygen by mesoporous etched halloysite nanotube supported Co.

One dimensional mesoporous etched halloysite nanotube supported Co is achieved by selective etching of AlO from halloysite nanotube (HA) and immersing the etched HA (eHA) into the Co(NO)·6HO solution consecutively. By facilely tuning the etching time and the weight ratio of Co(NO)·6HO to eHA, the morphology, specific surface area and the supported Co content of the mesoporous material can be tuned. The method for mesoporous material is scaled up and can be extended to other clay minerals.

[The changes of circulating follicular regulatory T cells and follicular T helper cells in children immune thrombocytopenia].

Objective: To investigate the changes and roles of follicular regulatory T cells (Tfr) and follicular T helper cells (Tfh) in the pathogenesis of children immune thrombocytopenia (ITP).

Methods: 32 untreated ITP patients, as well as 20 healthy controls were enrolled in this study. The proportion of circulating Tfr and Tfh cells were determined by flow cytometry; real-time PCR was performed to detect the expression of transcription factors and regulatory factors of Bcl-6, c-Maf, Blimp-1 and PD-1 mRNA; ELISA was used to detect plasma concentration of IL-2, IL-6, IL-10 and IL-21.

[Analysis of prognostic risk factors in childhood hemophagocytic syndrome].

Objective: To identify and explore the prognostic risk factors of the hemophagocytic syndrome (HPS).

Methods: A retrospective study was conducted on 50 childhood patients with HPS who were admitted to our hospital between 2007 and 2011. All their medical records were reviewed and analyzed.

Urinary angiotensinogen levels in relation to renal involvement of Henoch-Schonlein purpura in children.

Aim: To investigate whether urinary angiotensinogen (UAGT) levels are correlated with renal involvement of Henoch-Schonlein purpura (HSP) in children, and to explore whether UAGT has any relation to the severity of HSP.

Methods: The study sample consisted of 107 patients (50 boys and 57 girls, 6.68±2.

Relationship between NRAGE and the radioresistance of esophageal carcinoma cell line TE13R120.

Background And Objective: The mRNA levels of 59 genes, detected by cDNA microarray, were up-regulated in the radioresistant human esophageal cacinoma cell line TE13R120 as compared with its parental cell line TE13 before and after radiation, and the expression of NRAGE gene showed a gradually up-regulating tendency. This study aimed to further detect the differences of NRAGE gene and protein expression and apoptosis between TE13R120 and TE13 cells, and to investigate the relationship between the NRAGE and the radioresistance of TE13R120 cells and its mechanism.

Methods: The two cell lines were irradiated by ⁶⁰Co γ-ray at different conditions.