Translation of Mutant Repetitive Genomic Sequences in and Changes in the Secondary Structures and Functional Specifications of the Encoded Proteins.

Multiple repetitive sequences of authentic genes commonly exist in fungal genomes. AT-biased genotypes of have been hypothesized as repetitive pseudogenes in the genome of (GC-biased Genotype #1 of ) and are generated through repeat-induced point mutation (RIP), which is charactered by cytosine-to-thymine and guanine-to-adenine transitions, concurrent epigenetic methylation, and dysfunctionality. This multilocus study examined repetitive sequences in the genome and transcriptome using a bioinformatic approach and revealed that 8.

Analysis of Metabolic Profiles and Antioxidant Activity of Chinese Cordyceps, and Based on Untargeted Metabolomics.

Chinese cordyceps (GL) is a traditional medicinal fungus, with (, BL) and (, JSB) being fungi isolated from wild Chinese cordyceps. These three species share similar chemical composition and pharmacological effects. Existing studies have primarily compared the metabolites of Chinese cordyceps and , overlooking the assessment of antioxidant capacity in Chinese cordyceps, , and .

Effects of Lianhuaqingwen Capsules in adults with mild-to-moderate coronavirus disease 2019: an international, multicenter, double-blind, randomized controlled trial.

Background: In a randomized trial, Lianhuaqingwen (LHQW) capsule was effective for accelerating symptom recovery among patients with coronavirus disease 2019 (COVID-19). However, the lack of blinding and limited sample sizes decreased the level of clinical evidence.

Objectives: To evaluate the efficacy and safety of LHQW capsule in adults with mild-to-moderate COVID-19.

Bioinformatic Analysis of Secondary Metabolite Biosynthetic Potential in Pathogenic .

species are among the filamentous fungi with the most pronounced impact on agricultural production and human health. The mycotoxins produced by pathogenic not only attack various plants including crops, causing various plant diseases that lead to reduced yields and even death, but also penetrate into the food chain of humans and animals to cause food poisoning and consequent health hazards. Although sporadic studies have revealed some of the biosynthetic pathways of toxins, they are insufficient to satisfy the need for a comprehensive understanding of toxin production.

Altered GC- and AT-biased genotypes of Ophiocordyceps sinensis in the stromal fertile portions and ascospores of natural Cordyceps sinensis.

Objective: To examine multiple genotypes of Ophiocordyceps sinensis in a semi-quantitative manner in the stromal fertile portion (SFP) densely covered with numerous ascocarps and ascospores of natural Cordyceps sinensis and to outline the dynamic alterations of the coexisting O. sinensis genotypes in different developmental phases.

Methods: Mature Cordyceps sinensis specimens were harvested and continuously cultivated in our laboratory (altitude 2,254 m).

[Differential transcription of mating-type genes during sexual reproduction of natural Cordyceps sinensis].

Natural Cordyceps sinensis as an insect-fungal complex, which is developed after Ophiocordyceps sinensis infects a larva of Hepialidae family. Seventeen genotypes of O. sinensis have been identified in natural C.

Differential coexistence of multiple genotypes of Ophiocordyceps sinensis in the stromata, ascocarps and ascospores of natural Cordyceps sinensis.

Objective: To examine the differential occurrence of Ophiocordyceps sinensis genotypes in the stroma, stromal fertile portion (SFP) densely covered with numerous ascocarps, and ascospores of natural Cordyceps sinensis.

Methods: Immature and mature C. sinensis specimens were harvested.

Medium optimization for high mycelial soluble protein content of using response surface methodology.

is widely utilized due to its pharmaceutical value. Mycelial protein forms a key active component of and determines the medicinal potential of fungus. Here, we describe the development of an optimized fermentation medium to obtain more mycelial soluble protein from using response surface methodology (RSM) and investigate the increased mycelial protein content using transcriptomics.

Antibacterial activity and action target of phenyllactic acid against Staphylococcus aureus and its application in skim milk and cheese.

Phenyllactic acid (PLA) has been demonstrated to possess antibacterial activity and capacity to prolong food shelf life. However, studies on the performance of PLA in inhibiting Staphylococcus aureus and its effectiveness when applied to dairy products are largely lacking. Here, antibacterial activity (planktonic and biofilm states) of PLA against S.

A novel bacteriocin against Staphylococcus aureus from Lactobacillus paracasei isolated from Yunnan traditional fermented yogurt: Purification, antibacterial characterization, and antibiofilm activity.

Staphylococcus aureus and its biofilm have emerged as a significant threat to the safety of dairy products. In recent years, lactic acid bacteria (LAB) bacteriocins have been widely acknowledged as the potential natural antibacterial substance in food biopreservation due to their excellent antibacterial effects. However, few LAB bacteriocins with antibacterial and antibiofilm activity against S.

Starch-enhanced degradation of HMW PAHs by Fusarium sp. in an aged polluted soil from a coal mining area.

The present study used strain ZH-H2 (Fusarium sp.) isolated by our group as the PAH-degrading strain and 5-6-rings PAHs as degradation objects. The soil incubation experiment was carried out to investigate the starch-enhanced degradation effects of HMW PAHs by Fusarium sp.

Antifungal, Phytotoxic, and Cytotoxic Activities of Metabolites from Epichloë bromicola, a Fungus Obtained from Elymus tangutorum Grass.

The development of high-quality herbage is an important aspect of animal husbandry. Inoculating beneficial fungi onto inferior grass is a feasible strategy for producing new varieties of high-quality herbage. Epichloë bromicola is a candidate fungus that is isolated from Elymus tangutorum.

[Approach to the road of vigorously developing Chinese medicine by scientific location of channels and collaterals].

Through researches of channels and collaterals and clinical practice of many years, the authors understand that Chinese medicine, which considers the human body as an interrelated, mutual constraints, whole, dynamic living system, has gradually become an important part of modern medicine. Channels and collaterals are a closed loop system which is communicated and linked by energy and information in the form of electromagnetic oscillation, reflecting many characteristics similar to quantum. Channels and collaterals are not a fixed organizational structure.

[Meridian regulation is a course of communicating and implicating of the informational system with the material system].

Objective: To approach to the intrinsic mechanism of meridian regulation.

Methods: Summarize and analyze the results of the author's studies and the development of other studies about life regulating and meridian at home and abroad.

Conclusion: The regulating mechanism of meridians has two levels.

[Relationship of meridians with human material and energy systems study on theory and clinical application of meridians (IV)].

Objective: To expound the relationship between meridians and the human two information systems, material system and energy system.

Methods: Summarize and analyze the results of experimental and other studies.

Conclusion: The carrier of the meridian is human material system.

[Study on theory and clinical application of meridians (III)].

Objective: To probe into essence of meridians.

Methods: To examine meridians, acupoints, brain and relative organs by SQUID and fMRI, and to analyze the results.

Conclusion: A relative stable circular current of electromagnetic and chemical oscillation along the low electric resistance pathway was found.