[Detecting the concentrations of bisphenol A (BPA) in air of workplaces with HPLC].

Objective: To establish the method of detecting the concentrations of bisphenol A (BPA)in air of workplaces with high performance liquid chromatographic (HPLC).

Methods: According to standards of methods for determining the chemical substances in workplace air, BPA in the air was collected by glass fiber filter, then dissolved by acetonitrile and determined by high performance liquid chromatography with FLD.

Results: There was a linear relationship within the range of 0.

[Effects of applying controlled release fertilizers on N2O emission from a lateritic red soil].

Static closed chamber technique and contrast method were adopted to study the effects of three coated compound fertilizers (N:P2O5:K2O = 19:8.6:10.5, high N; 14.

Electroacupuncture at Qiuxu (GB 40) for treatment of migraine--a clinical multicentral random controlled study.

Objective: To observe the therapeutic effects of electroacupuncture (EA) at Qiuxu (GB 40) for treatment of migraine so as to provide clinical evidence for compilation of the Acupoints' Dictionary of the People's Republic of China.

Methods: 275 migraine patients admitted in 3 hospitals were randomly divided into a treatment group treated by EA at Qiuxu (GB 40), and a control group treated by EA at Tianshu (ST 25). The indexes of the migraine symptoms and the 5-HT level were observed in both the groups before and after treatment.

[Levels of environmental endocrine disruptors in umbilical cord blood and maternal blood of low-birth-weight infants].

Objective: To monitor the biological levels of environmental endocrine disruptors (EDs, phthalates and surfactants) in the umbilical cord blood and maternal blood of low-birth-weight infants.

Methods: All 30 umbilical cord blood samples and 21 maternal blood samples were collected from low-birth-weight infants. The concentration of four kinds of phthalates (di-2-ethylhexyl phthalate, DEHP; mono-2-ethylhexyl phthalate, MEHP; di-ethyl phthalate, DEP; di-n-butyl phthalate, DBP) and two kinds of surfactants (4-nonylphenol, 4-NP; octylphenol, OP) in these blood samples were measured by using reversed-phase high performance liquid chromatography.

[Study on the therapeutic effect of electroacupuncture on migraine and influencing factors].

Objective: To compare therapeutic effects of electrical acupuncture (EA) at Qiuxu (GB 40) and Tianshu (ST 25), and to probe the factors of influencing the therapeutic effect.

Methods: Two hundred and seventy-five cases were randomly divided into the test group of 138 cases treated with EA at Qiuxu (GB 40) and the control group of 137 cases treated with EA at Tianshu (ST 25). Their therapeutic effects were observed and the factors influencing the therapeutic effect were analyzed.

[Effect of dimethoate on serum monoamines neurotransmitters in rats].

Objective: To study the effect of dimethoate on the monoamine Neurotransmitters, including norepinephrine (NE), epinephrine (E), serotonin (5-HT), dopamine (DA) and its metabolite (3, 4-hydroxyphenylacetic acid, DOPAC) in the serum of rats and furthermore to explore the non-cholinergic mechanism of organophosphate induced toxicity.

Methods: Groups of rats were treated with saline and 38.9, 83.

Phthalate exposure and human semen quality in Shanghai: a cross-sectional study.

Objective: To monitor the level of phthalates in human semen samples and to analyze the relationship between phthalate levels and semen parameters.

Methods: Concentrations of three kinds of commonly used phthalates (di-ethyl phthalate, DEP; di-n-butyl phthalate, DBP; di-2-ethylhexyl phthalate, DEHP) were measured using reversed-phase HPLC. Semen parameters were measured by computer aided sperm analysis (CASA).

[Determination of time-weighted average concentration for vinyl chloride in the air of workplace].

Objective: To establish a gas chromatographic method with carbon disulfide desorption for time-weighted average (TWA) concentration of vinyl chloride in the air of workplace.

Methods: Vinyl chloride in the air was sampled individually with activated charcoal tube. Vinyl chloride was desorbed by carbon disulfide for 30 minutes and analyzed by GC (FID).

[Determination of four kinds of environmental endocrine disruptors in blood serum of precocious puberty patients by high performance liquid chromatography].

Objective: This study was directed to the development of a simple and highly sensitive method for determination of four kinds of environmental endocrine discrupters (EEDs) in the serum of precocious puberty patients using reversed-phase HPLC.

Methods: The serum samples were extracted by n-hexane. The operating conditions were such as C18 column (4.

[Determination of ergosterol amount in house dust of different indoor environment in Shanghai City by HPLC].

Objective: A high performance liquid chromatographic (HPLC) method was developed for the determination of the amount of ergosterol in house dust, and was examined the feasibility on determination of ergosterol in house dust. According to the established method, different house dusts derived from different functional indoor air was sampled and determined. We expected to regard ergosterol as an indicator of fungal biomass.

[Elevation of some serum liver enzymes in coke oven workers and the possible relationship with exposure to polycyclic aromatic hydrocarbons].

Objective: To explore the dose-response relationship between exposure to polycyclic aromatic hydrocarbons (PAHs) and the elevation of some serum liver enzymes in coke oven workers.

Methods: Cumulative exposure level of total 16 PAHs (CEL-PAHs) and urinary 1-hydroxypyrene (1-OHP) concentrations were assessed, liver enzymes including alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transpeptidase (GGT) and alkaline phosphatase (ALP) were determined between coke oven workers and non-occupational PAHs exposed workers with negative hepatitis B surface antigens (HBsAg).

Results: The exposure levels of PAHs in coke oven workers were significantly higher than in control group (P < 0.

[Study on monoamine neurotransmitters in rat serum and brain tissue by high performance liquid chromatography with electrochemical detection].

Objective: A rapid, reliable and simple method detecting monoamine neurotransmitters in rat serum and brain tissue by high performance liquid chromatography with electrochemical detector was developed.

Methods: An ODS column was selected as separation column at room temperature,and the mobile phase (pH4.50) consisted of 0.

[Study on the level of environmental endocrine disruptors in serum of precocious puberty patients].

Objective: To investigate the role of environmental endocrine disruptors (EEDs) in causing the precocious puberty.

Methods: The blood samples were collected from 79 cases of precocious puberty patients and 42 cases of normal children. The concentrations of 4-nonylphenol (4-NP), 1, 1-dichloro-2, 2, bis (p-chlorophenyl) ethylene (p, p'-DDE) and di-2-ethylhexyl phthalate (DEHP) in blood serum samples were measured by using reversed-phase high performance liquid chromatography (HPLC).

[Comparison on urinary 1-hydroxypyrene concentrations between coke oven workers and non-occupational exposed individuals].

Objective: To compare the urinary 1-hydroxypyrene (1-OHP) concentrations between coke oven workers and non-occupational exposed individuals and to investigate the possible impact factors.

Methods: Spot end-of-shift urine samples were collected in 265 coke oven workers and spot morning urine samples in 226 non-occupational exposed individuals. External exposure levels and possible confounding factors were assessed by environmental polycyclic aromatic hydrocarbons (PAH) monitoring and uniform questionnaire, and the urinary 1-OHP concentrations determined by high performance liquid chromatography (HPLC), were used to compare the different urinary 1-OHP levels between different research populations and to explore the effects of several factors such as external exposure levels, smoking habits, alcohol consumptions, age and body mass index (BMI).

[Survey on the contamination of microcystin-LR in water supply of Shanghai city].

Objective: To study the pollution level of microcystin-LR in water supply of Shanghai city and the removal efficacy for microcystin-LR through routine water treatment technique.

Methods: High performance liquid chromatogram (HPLC) was applied to determine the concentration of microcystin-LR in source water, water samples after various water treatment procedures and tap water.

Results: The concentration of microcystin-LR varied with sampling seasons and sites and reached peak during summer and fall.

[Occupational exposure profiles of polycyclic aromatic hydrocarbons in coke oven workers].

Objective: To study the characteristics of occupational exposure of polycyclic aromatic hydrocarbons (PAHs) in coke oven workers.

Methods: Samples were collected individually and PAHs concentration in the ambient air were assayed by high performance liquid chromatography (HPLC) and toxic equivalency factors (TEFs) were introduced to assess the carcinogenic potency.

Results: The levels of PAHs occupational exposure in oven workers at topside was higher than at side oven and bottom oven (P < 0.