[Association between expressions of HSP70, HSP90 and efficacy of chemotherapy in colorectal cancer patients with unresectable liver metastasis].

Objective: To investigate the association between expressions of HSP70, HSP90 and efficacy of chemotherapy in colorectal cancer patients with unresectable liver metastasis.

Methods: Data of 52 colorectal cancer cases, whose primary colorectal focuses were resected but hepatic metastatic tumors were unresectable, were reviewed retrospectively. All the patients underwent FOLFOX4 regimen well.

[A Novel Method for Increasing the Number of Metaphase Cells Cultured in vitro.].

To increase the number of metaphase cells cultured in vitro, two bovine fibroblast cell lines (YFF and CNF) were frozen at 4 degrees for different length of time prior to colchicine treatment , and then chromosomal specimen were prepared. The percentage of metaphase cells was examined under conditions above. Using this method, the variation rate of karyotype of YFF and CNF subcultured up to passage 20 were also analyzed.