A preoperative nomogram predicting risk of lymph node metastasis for early-stage cervical cancer.

Objective: This study aimed to develop a preoperative nomogram based on clinical and pathological characteristics to provide a more individualized and accurate estimation of lymph node metastasis (LNM) in patients with early-stage cervical cancer.

Methods: A total of 7,349 early-stage cervical cancer patients with pathologically confirmed between 1988 and 2015 were obtained from the Surveillance, Epidemiology, and End Results (SEER) database. All the patients were divided into training (n = 5,500) and validation (n = 1,849) cohorts randomly.

Transcription factors MdMYC2 and MdMYB85 interact with ester aroma synthesis gene MdAAT1 in apple.

Volatile esters in apple (Malus domestica) fruit are the critical aroma components determining apple flavor quality. While the exact molecular regulatory mechanism remains unknown, jasmonic acid (JA) plays a crucial role in stimulating the synthesis of ester aromas in apples. In our study, we investigated the effects of methyl jasmonate (MeJA) on the production of ester aroma in apples.

MdERF1B-MdMYC2 module integrates ethylene and jasmonic acid to regulate the biosynthesis of anthocyanin in apple.

Ethylene and jasmonic acid (JA) are crucial hormones that promote anthocyanin synthesis in apple ( × ) However, the mechanism by which these hormones cooperate to modulate anthocyanin production in apple is unclear. According to our results, expression was strongly induced by ethylene and JA. Physiological phenotypes and the results of molecular biological analyses indicated that encodes a positive regulator of anthocyanin synthesis.

Ethylene precisely regulates anthocyanin synthesis in apple via a module comprising MdEIL1, MdMYB1, and MdMYB17.

Ethylene regulates anthocyanin synthesis in ripening apple fruit via the antagonistic activities of the R2R3-MYB repressors and activators. However, the molecular mechanism underlying this process remains unknown. In this study, ethylene significantly induced the expression of the R2R3-MYB gene MdMYB17 in apple fruit.

Preparation of magnetic yolk-shell structured metal-organic framework material and its application in pharmacokinetics study of alkaloids.

In this study, a magnetic yolk-shell structured metal-organic framework material (FeO@YS-UiO-66-NH) is prepared by the directional etching of Co/peroxymonosulfate and in situ magnetization. The characteristic properties of the material were investigated by using field emission scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, X-ray powder diffraction, Fourier transform infrared spectroscopy, vibrating sample magnetometer, Brunauer-Emmett-Teller, and contact angle test. The FeO@YS-UiO-66-NH shows the advantages of large surface area, good magnetic property, and satisfactory stability, as well as giving high affinity to alkaloids (ALs) via hydrophilic interaction, hydrogen bonding, and π-π interaction.

Combination of mild therapeutic hypothermia and adipose-derived stem cells for ischemic brain injury.

Mild therapeutic hypothermia has been shown to mitigate cerebral ischemia, reduce cerebral edema, and improve the prognosis of patients with cerebral ischemia. Adipose-derived stem cell-based therapy can decrease neuronal death and infiltration of inflammatory cells, exerting a neuroprotective effect. We hypothesized that the combination of mild therapeutic hypothermia and adipose-derived stem cells would be neuroprotective for treatment of stroke.

A regulatory loop involving miR-29c and Sp1 elevates the TGF-β1 mediated epithelial-to-mesenchymal transition in lung cancer.

Specificity protein1 (Sp1) is required for TGF-β-induced epithelial-to-mesenchymal transition (EMT) which has been demonstrated to aggravate the progression of cancer including lung cancer. microRNA-29c (miR-29c) is identified to inhibit EMT, but the correlation between miR-29c and Sp1 in human lung cancer remain incompletely clarified. Here, we confirmed decreased expression of miR-29c and enhanced expression of Sp1 in lung cancer tissues (n = 20) and found that Sp1 could be targeted and inhibited by miR-29c.

Heart rate variability predicts mortality in peritoneal dialysis patients.

Background: The predictive value of heart rate variability (HRV) in peritoneal dialysis (PD) has never been tested.

Methods: In this study, the associations between HRV measures and the mortality in 81 PD patients were analyzed. HRV was measured by using 5-min recordings of a stationary system by a standardized method.

[Effect of prefrontal infralimbic cortex GABAA receptor agitating on passive avoidance memory consolidation of rats].

In this study, one-trial step-through task was applied to reveal the effect of prefrontal infralimbic cortex GABAA receptor agitating on passive avoidance memory consolidation in rats. Immediately after training (with shock), GABAA receptor agonist, muscimol (0.5 μg/0.

Heart rate variability in peritoneal dialysis patients: what is the role of residual renal function?

Background: The relationship between heart rate variability (HRV) and residual renal function (RRF) has not been elucidated previously.

Methods: In this cross-sectional study, HRV was evaluated in 71 peritoneal dialysis patients. Patients were divided into RRF decline group, RRF stable group and anuric group.

The study of spectral analysis of heart rate variability in different blood pressure types in euvolemic peritoneal dialysis patients.

Background: Heart rate variability (HRV) is an useful noninvasive tool to assess autonomic nervous system (ANS) function and may provide an insight into the understanding of the role of ANS in the pathogenesis of blood pressure (BP) abnormality in euvolemic continuous ambulatory peritoneal dialysis (CAPD) patients.

Methods: In this cross-sectional study, 62 CAPD patients with normal hydration values (assessed by a bioimpedance spectroscopy device) were enrolled from our peritoneal dialysis (PD) unit. Patients were divided into three groups according to their BP: normotension (NT), hypertension (HT), and hypotension (HyT).

[Effect of naoxintong capsule on the vascular endothelial function and the infarct size of patients with acute myocardial infarction].

Objective: To observe the effect of Naoxintong Capsule (NC) on the vascular endothelial function and the infarct size of patients with acute myocardial infarction (AMI).

Methods: One hundred and four patients with AMI were randomly assigned to the NC group (Group A, 36 cases), the Tongguan Capsule group (Group B, 32 cases), and the conventional Western medicine group (Group C, 36 cases). The conventional Western medicine was given to the three groups.

[Cross-sectional study of relation between blood pressure and heart rate variability in patients with peritoneal dialysis].

Objective: To analyze the possible relationship between heart rate variability (HRV) and blood pressure in peritoneal dialysis patients.

Methods: In this cross-sectional study, 93 continuous ambulatory peritoneal dialysis patients (CAPD) patients were enrolled. Patients were divided into three groups according blood pressure.

Effects of DAPT and Atoh1 overexpression on hair cell production and hair bundle orientation in cultured Organ of Corti from neonatal rats.

Background: In mammals, hair cells do not undergo spontaneous regeneration when they are damaged and result in permanent hearing loss. Previous studies in cultured Organ of Corti dissected from neonatal animals have shown that both DAPT (r-secretase inhibitor in the Notch signal pathway) treatment and Atoh1 overexpression can induce supernumerary hair cells. The effects of simultaneous DAPT treatment and Atoh1 over expression in the cells of cultured Organ of Corti from neonatal rats are still obscure.

The effect of blood pressure (37 vs 45 mmHg) and carotid occlusion duration (8 vs 10 min) on CA1-4 neuronal damage when using isoflurane in a global cerebral ischemia rat model.

This study presents our findings on the extent of neuronal damage in the hippocampal CA1-4 subfields following global (forebrain) cerebral ischemia in rats when using different blood pressure levels (37 vs 45 mmHg) and bilateral carotid occlusion durations (8 vs 10 min) under isoflurane anesthesia. We observed that global ischemia induced at a blood pressure of 37 mmHg resulted in high-grade CA1 neuron injury (>90%) at either duration of carotid occlusion. In contrast, global ischemia induced at a blood pressure of 45 mmHg resulted in either high-grade CA1 neuronal loss or a neuronal loss of ≈50% or less.

Comparison of the efficacy of mild hypothermia (35°c) and moderate hypothermia (33°c), alone or combined with magnesium treatment, when commenced 2 or 4 hours after global cerebral ischemia in rats.

We first assessed the neuroprotective efficacy of mild hypothermia (35°C/24 hours) alone and in combination with magnesium (intravenous loading dose: 360 μmol/kg; 48 hours infusion: 120 μmol/kg/h) commencing 4 hours after global ischemia. Treatment with mild hypothermia alone (CA1 survival rate: 8.7%±0.

[Application of near-infrared absorption spectrum scanning techniques in gas quantitative measurement].

A practical gas sensing system utilizing absorption spectrum scanning techniques was developed. Using the narrow-band transmission of a fiber tunable filter (TOF) and wavelength modulation technique, the so-called cross-sensing effects of the traditional spectrum absorption based gas sensor were reduced effectively and thus the target gas was detected sensitively and selectively. In order to reduce the effects of nonlinearity of TOF on the measurement results and improve the system stability in operation, the reflection spectrum of a reference FBG was monitored and employed to control the modulation region and center of TOF wavelength precisely.

[Application of PSO algorithm in wavelength detection of FBG sensors].

In order to improve the measurement accuracy of FBG sensing system, particle swarm optimization (PSO) algorithm combined with reference FBGs array was applied to investigate the nonlinearity and hysteresis character of Fabry-Parot filter (FPF). A method of modeling the wavelength-voltage relationship of FPF online in each FPF scanning cycle was proposed in the present paper. The feature of particle swarm optimization algorithm such as fast convergence and simple implementation makes the process of modeling wavelength-voltage relationship of FPF be completed with low computing cost and high accuracy.

[Effects of climate change on photosynthesis of Diospyros kaki under different soil moisture conditions].

Taking two-year-old Diospyros kaki as test material, this paper studied the effects of high CO2 concentration (700 micromol x mol(-1)), high temperature (5 degrees C higher than the mean daily temperature); and their combination on the net photosynthesis rate (Pn), evapotranspiration (Tr), stomatal conductance (Gs), water use efficiency (WUE), chlorophyll content, Fv/Fm, and Fv/Fo under different soil moisture conditions, with the ambient air temperature and CO2 concentration (380 micromol x mol(-1)) as the control. Under all test soil moisture conditions, the combination of high CO2 concentration and high temperature decreased the Tr and Gs, but increased the WUE. This combination increased the Pn when the soil moisture content was 75%-85% and 55%-65% of field capacity, but decreased the Pn when the soil moisture content was 35%-45%.

[The relation between en-dormancy and changes in two main electron transport pathways of nectarine (Prunus persica var. nectariana) buds].

Respiration rate of buds of Prunus persica var. nectariana Maxim. cv.

Application of real-time fluorescence quantitative PCR to rapid molecular detection of Down's syndrome.

Objective: To develop a rapid and reliable technique for the detection of Down's syndrome.

Methods: The peripheral blood samples were collected from twenty-five Down's syndrome patients and fifty normal individuals. Four polymorphic loci on chromosomes 21, 1, 19 were amplified by real-time fluorescence quantitative PCR, and then four pairs of deltaCt values were analytically compared between the two groups.

[Follow-up of 3 605 cord blood donors after 6 months of cord blood donation in Guangzhou cord blood bank].

To study the program of evaluating mothers and infants after 6 months of cord blood donation, from June 1998 to February 2004, all mothers after 6 months of cord blood donation were followed-up by phone calls or letters to report on the health condition. The results showed that when 3 195 mothers were visited by phone calls, 18 mothers declined to answer. 392 letter were send to those who could not be found by phone, 15 of whom wrote back.