[Effect of electroacupuncture of "Neiguan" (PC 6) on heart rate and plasma catecholamine contents in ventricular tachycardia rats].

Objective: To observe the effect of electroacupuncture (EA) of "Neiguan" (PC 6) on heart rate (HR) and plasma catecholamine (CA) levels in ventricular tachycardia (VT) rats.

Methods: A total of 50 SD rats were randomly divided into normal control, sham-operation (sham), model, EA-PC 6 and EA-LU 7 groups, with 10 cases in each. VT model was established by intravenous injection of cesium chloride (CsCl) via femoral vein.

[Effect of benzene on sperm DNA].

Objective: To assess the effect of benzene on sperm DNA.

Methods: Twenty-seven workers exposed to benzene for over two years were included in the experiment group and 35 unexposed ones were chosen as controls. Damage by benzene on sperm DNA was detected by modified single cell gel electrophoresis (SCGE).

[Detection of numerical aberrations of chromosomes 7 and 8 in sperms of workers exposed to benzene series by two-color fluorescence in situ hybridization].

Objective: To investigate the frequency of numerical aberrations for chromosomes 7 and 8 in the sperms of workers exposed to benzene series.

Methods: Numerical aberrations in the sperms of workers were detected by two-color fluorescence in situ hybridization with biotin labeled chromosome 7 probe (D7Z1) and digoxingenin labeled chromosome 8 probe (D8Z1).

Results: The time-weight average air concentration (TWA) of benzene in the workshop was 42.

[Detection of the frequencies of numerical and structural chromosome aberrations in sperm of benzene series-exposed workers by multi-color fluorescence in situ hybridization].

To study the frequencies of numerical and structural aberrations for chromosome in sperm of benzene exposed workers, the multi-color FISH was used. Four DNA probes(one for chromosome 1 centromere and one for 1 p terminal, and two for chromosome 18 centromere) were hybridized with interphase sperms, and the frequencies of numerical aberrations for chromosome 1, 18 and structural aberrations of chromosome 1 were detected simultaneously. The time weighted average concentration (TWA) of benzene in workplace (42.

Exposure to acrylonitrile induced DNA strand breakage and sex chromosome aneuploidy in human spermatozoa.

To explore acrylonitrile (ACN)-induced DNA strand breakage and sex chromosome aneuploidy in human spermatozoa, semen parameters were examined among 30 acrylonitrile-exposed workers according to WHO laboratory manual for the examination of human sperm. DNA strand breakage of sperm cells was investigated among 30 ACN-exposed workers using single cell gel electrophoresis (SCGE). The frequency of sex chromosome aneuploidy in sperm cells was analyzed among nine ACN-exposed workers using fluorescence in situ hybridization (FISH).

[Freezing effect on sperm DNA].

Objectives: To assess the effect of freezing on the sperm DNA.

Methods: To assess the sperm DNA preserved at -80 degrees C by using the single cell gel electrophoresis (SCGE).

Results: There was no statistical difference on the time factor by analysis of variance (ANOVA).