This work aimed to investigate the pharmacokinetics of six major blood-entering components(triptolide, triptophenolide, wilforgine, wilforine, tripterine, and wilforlide A) in Tripterygium wilfordii Polyglycosides Tablets(TWPT) between normal rats and the rat model of adjuvant arthritis, which was established by injection of Freund's complete adjuvant. Liquid chromatography-mass spectrometry(LC-MS) was employed to determine the content of six main components in the serum samples of normal and arthritic rats after administration of TWPT, and the pharmacokinetics of the six components were compared between normal and model rats. The specificity, linearity, accuracy, precision, stability, extraction recovery and matrix effect of the established LC-MS method all met the requirements.
View Article and Find Full Text PDFFive new compounds, including two sesquiterpenoid glycosides (1 and 2), two monoterpenoid glycosides (3 and 4), and a quinovose ester (5), together with four known compounds (6-9) were isolated from branches and leaves of Pittosporum pulchrum Gagnep. Their structures were established by 1D and 2D NMR, HR-ESI-MS, IR and UV spectral analyses. This is the first time to investigate the chemical constituents of P.
View Article and Find Full Text PDFNeuropathic pain(NP) is difficult to be treated since it has similar phenotypes but different pathogenesis in different pathological stages. Targeted intervention of the core regulatory elements at different pathological stages of NP has become a new direction of drug research and development in recent years and provides the possibility for the treatment of NP. The Mongolian medicine Naru-3(NR-3) is effective in the treatment of sciatica and trigeminal neuralgia, the mechanisms of which remain unknown.
View Article and Find Full Text PDFThe Baimai Ointment with the effect of relaxing sinew and activating collaterals demonstrates a definite effect on Baimai disease with pain, spasm, stiffness and other symptoms, while the pharmacodynamic characteristics and mechanism of this agent remain unclear. In this study, a rat model of chronic compression of L4 dorsal root ganglion(CCD) was established by lumbar disc herniation, and the efficacy and mechanism of Baimai Ointment in the treatment of CCD were preliminarily explored by behavioral tests, side effect evaluation, network analysis, antagonist and molecular biology verification. The pharmacodynamic experiment indicated that Baimai Ointment significantly improved the pain thresholds(mechanical pain, thermal pain, and cold pain) and gait behavior of CCD model rats without causing tolerance or obvious toxic and side effects.
View Article and Find Full Text PDFNeuropathic pain(NP) has similar phenotypes but different sequential neuroinflammatory mechanisms in the pathological process. It is of great significance to inhibit the initiation of neuroinflammation, which has become a new direction of NP treatment and drug development in recent years. Mongolian drug Naru-3 is clinically effective in the treatment of trigeminal neuralgia, sciatica, and other NPs in a short time, but its pharmacodynamic characteristics and mechanism of analgesia are still unclear.
View Article and Find Full Text PDFThe present study aimed to investigate the protective role of ergosterol, isolated from Scleroderma polyrhizum Pers., in lipopolysaccharide (LPS)-induced acute lung injury (ALI). ALI was induced in mice by LPS (0.
View Article and Find Full Text PDFApigenin (Api), a mainly bioactive component of Apium graveolens L. var. dulce DC.
View Article and Find Full Text PDFTwo new oxomolybdenum(VI) complexes [(TpMoO(2))(2)(μ-O)]·H(2)O (1) and (Tp(4-I)MoO(2))(2)(μ-O) (2) were synthesized and characterized by elemental analysis, IR spectra, UV-Vis spectroscopy, powder X-ray diffraction, single-crystal X-ray diffraction and thermogravimetric analyses (TG). In addition, the catalytic performances of complexes 1 and 2 were studied firstly on the oxidation reaction of cyclohexane at room temperature.
View Article and Find Full Text PDFJ Chromatogr B Analyt Technol Biomed Life Sci
March 2003
A chromatographic method for the separation and determination of D- and L-thyroxine enantiomers (D-, and L-T4) in human serum with a chiral ligand ion-exchange system using a chiral mobile phase additive and a silica column was established. An aqueous eluent containing L-proline (L-pro) sufficiently complexed copper II ions and triethylamine (TEA) was used. It was monitored with a UV detector.
View Article and Find Full Text PDFJ Chromatogr B Analyt Technol Biomed Life Sci
June 2002
A simple restricted-access media (RAM) HPLC method for simultaneous determination of the lactone and carboxylate forms of 10-hydroxycamptothecin (HCPT) in human serum was established. Using a RAM Hisep analytical column, serum samples were directly injected into the HPLC system. The eluted peaks of two forms of HCPT were monitored with a fluorescence detector.
View Article and Find Full Text PDF