[Distribution Characteristics and Formation Mechanism of Main Hydrochemical Ions in Qingshui River].

Qingshui River is a vital source for human life and industrial production in Zhangjiakou City. Determination of the formation mechanism of the main hydrochemical ions is important for the sustainable development and utilization of surface water resources in the Qingshui River. In view of this, 20 surface water samples were collected from the agricultural and urban reaches of the Qingshui River in July 2022.

Downregulation of human epidermal growth factor receptor 2 by short hairpin RNA increases chemosensitivity of human ovarian cancer cells.

The aim of the current study was to investigate the suppressive effects of pSilencer T7-human epidermal growth factor receptor 2 (HER2)-short hairpin RNA (shRNA) recombinant plasmids on human SKOV3 ovarian cancer cell growth and sensitivity to carboplatin (CBP). Three different pairs of shRNAs (shRNAa, shRNAb and shRNAc), targeting the HER2 gene, were selected and transfected into human SKOV3 cells, respectively. The expression levels of HER2 were then detected by immunohistochemical (IHC), semi-quantitative reverse transcription-polymerase chain reaction and western blot analyses.