[Construction and application of inducible artificial microRNA expression vector targeting eIF3g gene].

Aim: To construct an inducible artificial microRNA expression vector targeting eIF3g gene and use it to inhibit the expression of eIF3g in K562 cells.

Methods: The microRNA targeting human eIF3g was designed and obtained by PCR. After confirmed by sequencing, the microRNA was cloned into the pRevTRE2 plasmid.

[Construction and application of an artificial microRNA expression vector for inhibiting PAR4].

Aim: To construct artificial microRNA expression vector targeting PAR4 and suppress the expression of PAR4 in human colorectal cancer SW620 cells with the artificial microRNA.

Methods: Artificial microRNA was designed and amplified by two rounds of PCR and cloned into pMD-19T vector. The sequence of the cloned artificial microRNA was verified by DNA sequencing.