Decreased salivary α-amylase activity responding to citric acid stimulation in Myasthenia gravis with malnutrition.

Objectives: Malnutrition, defined according to Nutritional risk screening (NRS 2002), is commonly observed in patients of Myasthenia gravis (MG), a neuromuscular disorder manifested by varied degrees of skeletal muscle weakness. Because biochemical composition of saliva changes in correspondence to alterations in nutritional status, we tested our hypothesis that a certain saliva component(s) might serve as a biomarker(s) for nutrition status of MG, particularly for those MG patients with high risk of malnutrition.

Materials And Methods: 60 MG patients and 60 subjects belonging to the healthy control group (HCG) were enrolled in this case-control study.

Association between serum vitamin D levels and cytotoxic-associated gene A seropositivity: a cross-sectional study in US adults from NHANES III.

Objective: To assess the association of serum vitamin D (VD) levels and () cytotoxic-associated gene A (CagA) seropositivity, and further explore potential effect modifiers in this association.

Design: Cross-sectional study.

Setting: Data from phase I of the National Health and Nutrition Examination Survey (NHANES III, 1988-1991) led by the Center for Disease Control and Prevention.

The Single-Nucleotide Polymorphism of miR-27a and the Expression of miR-27a in -Related Diseases and the Correlation with the Traditional Chinese Medicine Syndrome.

Aims: The study aims to explore the effects of the single-nucleotide polymorphism of miR-27a and its expression in -related diseases and the relationship between gastric pathology and traditional Chinese medicine (TCM).

Methods: Subjects were classified into six histopathological groups and five TCM syndrome groups. All specimens underwent detection through rapid urease test and methylene blue staining.

Efficacy and safety of Qingre-Chushi therapies in active ulcerative colitis: A network meta-analysis.

Background: Ulcerative colitis (UC) is a chronic inflammatory disease with an increasing incidence in the world. Qingre-Chushi therapies (QC) can alleviate clinical symptoms. Therefore, a network meta-analysis was conducted to systematically evaluate the efficacy and safety of QC in the treatment of active UC patients.

Pharmacological treatments of Chinese herbal medicine for irritable bowel syndrome in adults: A network meta-analysis of randomized controlled trials.

Introduction: Plenty of clinical studies have suggested the value of Chinese herbal medicine (CHM) for patients with irritable bowel syndrome (IBS), but their efficacy and safety have not been systematically concluded yet. This article aimed to compare and rank the therapeutic effect and safety of CHM with routine pharmacotherapies and placebo in the treatment of IBS.

Methods: Randomized controlled trials regarding CHM to treat IBS were searched in six databases from inception to Jan 31, 2020.

Constraint-Induced Movement Therapy Promotes Neural Remodeling and Functional Reorganization by Overcoming Nogo-A/NgR/RhoA/ROCK Signals in Hemiplegic Cerebral Palsy Mice.

. Little is known about the induction of functional and brain structural reorganization in hemiplegic cerebral palsy (HCP) by constraint-induced movement therapy (CIMT). .

Efficacy and safety of non-pharmacological interventions for irritable bowel syndrome in adults.

Background: Although nonpharmacological interventions (NPI) for irritable bowel syndrome (IBS) have been applied clinically, their relative efficacy and safety are poorly understood.

Aim: To compare and rank different NPI in the treatment of IBS.

Methods: Five electronic databases were searched from their inception to January 12, 2020.

Different Traditional Herbal Medicines for the Treatment of Gastroesophageal Reflux Disease in Adults.

Background/aims: Traditional Herbal Medicines (THM) have been being used for gastroesophageal reflux disease (GERD) for a long time, but clinical evidence is still scarce. We evaluated different THM prescriptions for GERD in adults.

Methods: Data added to nine online databases from their inception to November 30, 2019, were systematically searched.

Interaction of Cyclooxygenase-2 with Induces Gastric Chronic Nonresolving Inflammation and the Formation of Syndrome of Internal Block of Static Blood in -Related Gastric Diseases.

Cyclooxygenase-2 (COX-2) is an inducible enzyme stimulated by various inflammatory factors (IFs). Chronic gastritis is a classic model of "inflammation-cancer transformation" and -related gastric diseases (HPGD) are specific ones of this model. Traditional Chinese Medicine (TCM) syndromes could play a predictive role in gastric histopathological evolution.

Efficacy of Jianpi Liqi therapy for functional dyspepsia: A meta-analysis of randomized, positive medicine-controlled trials.

Background: We performed this meta-analysis to assess the efficacy and safety of Jianpi Liqi therapy (JLT), a traditional Chinese medicine therapy, in treating functional dyspepsia (FD).

Methods: We systematically searched 13 databases from their inception to 15th, May 2019. Eligible studies were randomized controlled trials (RCTs) that compared JLT medicine with conventional pharmacotherapy (CP) in treating patients with FD.

Abnormalities in acute salivary biochemical characteristic responses to gustatory stimulation with citric acid in chronic non-atrophic gastritis.

Background And Aim: Salivary characteristics are altered in gastrointestinal diseases and related to oral taste disorder. However, specific salivary biochemical characteristics and their relationships with oral taste disturbances in chronic non-atrophy gastritis (CNAG) remain uncertain.

Methods: Seventy patients with CNAG and 70 subjects in healthy control group (HCG) were enrolled in our study.

Systematic review and meta-analysis of traditional Chinese medicine in the treatment of constipation-predominant irritable bowel syndrome.

Aim: This meta-analysis analyzed the efficacy and safety of traditional Chinese medicine (TCM) for the treatment of irritable bowel syndrome with constipation (IBS-C).

Methods: We searched seven electronic databases for randomized controlled trials investigating the efficacy of TCM in the treatment of IBS-C. The search period was from inception to June 1, 2017.

Gender differences in the saliva of young healthy subjects before and after citric acid stimulation.

Background: Gender differences in the function and anatomical features of salivary glands are well known. However, specific gender differences in the biochemical composition and salivary flow rate (SFR) remain uncertain. Collection methods affect the assessment of the salivary composition and SFR, which are also highly affected by acid stimulation.

[Effects of Methanol Extracts from Atractylodes macrocephalae Rhizoma on Small Intestinal Epi- thelial Cell Proliferation and Migration, and Expression of Phospholipase C-γl].

Objective: To observe the effects of methanol extracts from Atractylodes macro- cephalae Rhizoma (AMR) on the proliferation and migration of IEC-6 cell (small intestinal epithelial cells) and the expression of phospholipase C-γ1 (PLC-γ1) , and to explore the mechanism of AMR (a Chinese herb capable of invigorating Pi replenishing qi) for promoting repair of gastrointestinal mucosal injury.

Methods: IEC-6 cells were divided into the blank group, the positive control (spermidine, SPD; 5 μmol/L) group, AMR extracts groups (50, 100, and 200 mg/L). The alpha-difluoromethylornithine (DFMO, polyamines synthesis inhibitor) group, the SPD +DFMO group, AMR extracts (50, 100, and 200 mg/L) +DF- MO groups were set up in stress test.

[Effect of Sijunzi Decoction Polysaccharide on IEC-6 Cell Migration,Potassium Channel and Membrane Potential].

Objective: To investigate the effect of Sijunzi decoction polysaccharide( SJZDP) on intestinal epithelial cells( IEC-6)cell migration and polyamine signaling pathway potassium channel during intestinal epithelial cell migration, and to explore the mechanism of SJZDP on promoting gastrointestinal mucosal restitution after wounding.

Methods: Cell migration model was established by scratch damage, and then the effect of SJZDP normal cultured or with difluoromethylornithine( DFMO) and 4-aminopyridine( 4-AP) on IEC-6 cell migration was observed and calculated on this wounding model. The effect of SJZDP on expression of IEC-6 cell kv1.

[Comparative Study on Effects of Anti-Gastritis,Gastric Mucosal Protection and Gastrointestinal Movement Promotion of Root and Stem of Zanthoxylum nitidum].

Objective: To provide the experimental evidence for expansion of medicinal parts of Zanthoxylum nitidum by comparing the effects of anti-gastritis,gastric mucosal protection and gastrointestinal movement promotion of its root and stem.

Methods: The pharmacological effects between root and stem of Zanthoxylum nitidum were compared by observing the anti-gastritis effect on rats with chronic superficial gastritis induced by iodoacetamide, evaluating the gastric mucosal protective effect on rats’ gastric ulcer induced by stress, indometacin and pylorus ligation test, and investigating gastrointestinal movement promotion effect on mice gastric evacuation and intestinal propelling.

Results: Both root and stem of Zanthoxylum nitidum showed effects of relieving the inflammation symptoms of rats’ gastric mucosa induced by iodoacetamide, gastric ulcer respectively induced by stress, and presenting a strong inhibition of free acid and pepsin activity in gastric juice.

[Effect of 3 saliva collection methods on salivary secretion].

Purpose: This study compared the effect of 3 saliva collection methods on salivary secretion, in order to select optimum collection method for follow-up studies.

Methods: Fifty-five young healthy volunteers' saliva samples were collected by EP tube collecting emulated with natural flow (ETC), rotating mouth swab slightly (RMS) and chewing mouth swab (CMS) before and after stimulating with acid. The salivary flow rate, salivary alpha-amylase (sAA) activity of each saliva sample and its ratio before and after stimulating with acid were detected to provide the basis for the preferred method of collecting saliva.

Atractylodes macrocephala Koidz stimulates intestinal epithelial cell migration through a polyamine dependent mechanism.

Ethnopharmacological Relevance: Atractylodes macrocephala Koidz (AMK), a valuable traditional Chinese herbal medicine, has been widely used in clinical practice for treating patients with disorders of the digestive system. AMK has shown noteworthy promoting effect on improving gastrointestinal function and immunity, which might represent a promising candidate for the treatment of intestinal mucosa injury. The aim of this study was to investigate the efficacy of AMK on intestinal mucosal restitution and the underlying mechanisms via intestinal epithelial (IEC-6) cell migration model.

Atractylodes macrocephala Koidz promotes intestinal epithelial restitution via the polyamine--voltage-gated K+ channel pathway.

Ethnopharmacological Relevance: Atractylodes macrocephala Koidz (AMK) has been used widely as a digestive and tonic in traditional Chinese medicine. AMK has shown noteworthy promoting effect on intestinal epithelial cell migration, which might represent a promising candidate for the treatment of intestinal mucosa injury. The aim of this study was to investigate the efficacy of AMK on intestinal mucosal restitution and the underlying mechanisms via IEC-6 cell migration model.

[Effect of polysaccharides from Radix Glycyrrhizae on migration and polyamines contents of IEC-6 cell].

Objective: To study the effect of polysaccharides from Radix Glycyrrhizae on migration and polyamines (putrescine, spermidine and spermine) contents of IEC-6 cell.

Methods: Cell migration model was induced by scratch method in each well,and the polyamines in IEC-6 cell was determined by pre-column derivation high performance liquid chromatography. The polysaccharides inhibited effect on migration and polyamines contents of IEC-6 cells, and on IEC-6 cell migration by DFMO (a polyamines synthesis inhibitor) and the polyamines contents in the cells were observed.

[Effect of sijunzi decoction on salivary amylase secretion disorder and VIP-cAMP signaling pathway in splenasthenic rats].

Objective: To observe the effect of Sijunzi Decoction on secretion disorder of salivary amylase in splenasthenic rat and its mechanism.

Methods: The model group rats received reserpine 0.5 mg/kg through subcutaneous injection while the control group rats received the same volume of saline for 8 days.

Docosahexaenoic acid suppresses the expression of FoxO and its target genes.

Docosahexaenoic acid (DHA), an n-3 polyunsaturated fatty acid, has previously been shown to ameliorate obesity-associated metabolic syndrome. To decipher the mechanism responsible for the beneficial effects of DHA on energy/glucose homeostasis and the metabolic syndrome, 30 weaned cross-bred pigs were randomly assigned to three groups and fed ad libitum with a standard diet supplemented with 2% of beef tallow, soybean oil or DHA oil for 30 days, and the gene expression profile of various tissues was evaluated by quantitative real-time polymerase chain reaction. The DHA-supplemented diets reduced the expression of forkhead box O transcription factor (FoxO) 1 and FoxO3 in the liver and adipose tissue.

Studies on cell migration model in intestinal epithelial restitution for pharmacological research.

Objective: To set up a suitable IEC-6 migration model for pharmacological research and observe the effect of complex polysaccharide from Astragalus membranaceus (Fisch.) Bge. var.

[Study on ribosomal protein gene expression in patients with ulcerative colitis of Pi-asthenic syndrome and Pi-asthenic syndrome].

Objective: To study differential expression profiles of ribosomal protein (RP) genes in healthy subjects and ulcerative colitis (UC) patients of Pi-asthenic syndrome (PAS) and of dampness-heat syndrome (DHS), thus providing experimental bases for " Pi as the source of qi and blood" theory from the view of protein synthesis.

Methods: RP genes arrays were made. The mucous membrane of colon was detected in four UC patients of PAS (UC-PAS), four UC patients of DHS (UC-DHS), and four healthy subjects (N), and data analyzed using BRB-TOOL Software Package (3.