Bioinformatics analysis of a whole genome sequence coupled with HPLC-DAD analysis revealed that sp. Hu103 has the capacity to produce skyllamycin analogues. A subsequent chemical investigation of this strain yielded four new cinnamoyl-containing cyclopeptides, anulamycins A-D (-), two new cinnamoyl-containing linear peptides, anulamycins E and F ( and ), and two known cyclopeptides, skyllamycins A () and B ().
View Article and Find Full Text PDFCarya cathayensis Sarg. (Chinese hickory) is one of the important economic forest plants, mainly distributed in Zhejiang and Anhui provinces in China. In September 2020, leaf spot disease occurred on 90% C.
View Article and Find Full Text PDFPurpose: We aim to investigate the potential role and underlying mechanisms of linc00174 on pyroptosis in the pathogenesis of DR.
Methods: Expression patterns of linc00174, miR-26a-5p and PTEN in human retinal microvascular endothelial cells (hRMECs) were detected by quantitative real-time PCR (qRT-PCR) and Western blot, respectively. Biological functions of linc00174 on cell proliferation and pyroptosis were evaluated by CCK-8, flow cytometry, caspase-1 activity assays, respectively.
Two new 22-membered macrolide metabolites, phthoramycins B (1) and C (2), were isolated from the fermentation broth of Streptomyces sp. HU210. Their structures were elucidated based on extensive spectroscopic techniques including 1D, 2D NMR and HRESIMS data.
View Article and Find Full Text PDFBackground: Paraganglioma occurring at the lateral skull base is a rare tumor. Surgery is the primary treatment of benign paragangliomas. Postoperative infection of the surgical site at the lateral skull base is very dangerous and hard to manage.
View Article and Find Full Text PDFBackground: A paucity of strategies exist for extensive-stage small cell lung cancer (ES-SCLC) patients who fail the first-line chemotherapy. Apatinib is a tyrosine kinase inhibitor (TKI) that selectively inhibits vascular endothelial growth factor receptor-2 (VEGFR-2), which has been demonstrated to have active anti-tumor activity in ES-SCLC when used only or combined with PD-1 inhibitors or chemotherapy with good tolerance. However, the efficacy and safety of apatinib monotherapy is unclear in second-line or beyond treatment of ES-SCLC.
View Article and Find Full Text PDFA chemical investigation of sp. Hu186 afforded two known quinone antibiotics, sarubicin A () and sarubicin B (), together with three unusual variants, sarubicinols A-C (-), and two new 1,4-naphthoquinone metabolites, sarubicin B () and sarubicin B (). Compounds - possess a rare 2-oxabicyclo [2.
View Article and Find Full Text PDFA new -class milbemycin, 13hydroxy milbemycin (), was isolated from the fermentation broth of a mutant of genetically engineered strain AVE-H39. Its structure and absolute configuration were elucidated by extensive spectroscopic methods and confirmed by single crystal X-ray diffraction.
View Article and Find Full Text PDFZika virus (ZIKV; ) is a devastating virus transmitted to humans by the mosquito Aedes aegypti. The interaction of the virus with the mosquito vector is poorly known. The double-stranded RNA (dsRNA)-mediated interruption or activation of immunity-related genes in the Toll, IMD, JAK-STAT, and short interfering RNA (siRNA) pathways did not affect ZIKV infection in A.
View Article and Find Full Text PDFBackground: High-temperature stress (HTS) is one of the main environmental stresses that limit plant growth and crop production in agricultural systems. Maca (Lepidium meyenii) is an important high-altitude herbaceous plant adapted to a wide range of environmental stimuli such as cold, strong wind and UV-B exposure. However, it is an extremely HTS-sensitive plant species.
View Article and Find Full Text PDFJ Asian Nat Prod Res
September 2021
Two new milbemycin metabolites, 13-hydroxymilbemycin () and 26-methyl-13-hydroxymilbemycin (), were isolated from the fermentation broth of a genetically engineered strain AVE-H39. Their structures were determined by the comprehensive spectroscopic data, including 1 D, 2 D NMR, MS spectral analysis and the comparison with data from the literature. Compounds and not only exhibited potent acaricidal activities against , but also had nematocidal activity against .
View Article and Find Full Text PDFMultiple daily insulin injections have been a common regimen worldwide for the management of diabetes mellitus but involved potential safety and compliance problems. In this context, a single integrated microneedle patch (IMP) with multiple release kinetics is demonstrated to provide better physiologic insulin coverage for postprandial glycemic excursion in a convenient and pain-free manner. The combination of rapid separating technique and multiple individual microneedle arrays provides the combined ability to efficiently deliver insulin into the skin within seconds and to independently control insulin release kinetics.
View Article and Find Full Text PDFThe screening and identification of hyperaccumulators is the key to the phytoremediation of soils contaminated by heavy metal (HM). Arbuscular mycorrhizal fungus (AMF) can improve plant growth and tolerance to HM; therefore, AMF-assisted phytoextraction has been regarded as a potential technique for the remediation of HM-polluted soils. A greenhouse pot experiment was conducted to determine whether Sphagneticola calendulacea is a Cd-hyperaccumulator and to investigate the effect of the AMF-Funneliformis mosseae (FM) on plant growth and on the accumulation, subcellular distribution and chemical form of Cd in S.
View Article and Find Full Text PDFJ Asian Nat Prod Res
July 2021
Two new milbemycin derivatives, milbemycin M () and milbemycin N (), were isolated from the culture of a genetically engineered strain BCJ60. Their structures were elucidated through the interpretation of NMR and HR-ESI-MS spectroscopic data, as well as comparison with previous reports. The acaricidal and nematicidal activities of them against and were tested.
View Article and Find Full Text PDFTwo new threonine-containing metabolites, -[4-hydroxy-3-prenyl-benzoyl]-L-threonine () and -[2,2-dimethyl-2-chromene-6-carbonyl]-L-threonine (), were isolated from the fermentation broth of the soil fungus strain HS-FG-257. Their structures were elucidated through the interpretation of HR-ESIMS and extensive NMR spectroscopic data. Both compounds exhibited no cytotoxic activity against the test cell lines A549 and HCT-116.
View Article and Find Full Text PDFTwo novel milbemycin derivatives, 5,27-epoxy-13α-hydroxy milbemycin β (1) and 5,27-epoxy-13α-hydroxy-25-ethyl milbemycin β (2), were isolated from the genetically engineered strain Streptomyces avermitilis AVE-H39. Their structures were elucidated through the interpretation of HR-ESIMS and extensive NMR spectroscopic data. Compounds 1 and 2 exhibited moderate acaricidal and nematicidal activities.
View Article and Find Full Text PDFStudies of maternal oral contraceptive pill (OCP) exposure and the offspring's risk of atopic diseases are of current interest due to concerns about widespread use of OCP before or during pregnancy.We evaluated whether maternal OCP exposure is associated with an increased risk of atopic diseases by reviewing the literature and performing a meta-analysis. The PubMed and Embase databases were searched to identify potential studies for inclusion.
View Article and Find Full Text PDFNovel antilipid peroxidative carbazole alkaloids, antiostatin A (), antiostatin A (), and (±)-morindolestatin (), were isolated from a new soil-derived sp. Compound possesses an unusual cyclohexene side chain. Compound was a pair of enantiomers featuring an unprecedented [1,4]oxazino[2,3-]carbazole ring system.
View Article and Find Full Text PDFACS Appl Mater Interfaces
November 2019
Poly(acrylic acid) (PAA) hydrogels with a multi-bond network composed of sparse chemical cross-links and carboxyl-Fe coordination are prepared through a controllable permeation strategy utilizing ferric citrate (FeCA). The existing strategies that directly soak PAA hydrogels in Fe solutions usually induce an inhomogeneous network with densely cross-linked shells and uncertain water content of the hydrogels, which brings about ambiguity when investigating strengthening mechanisms because water content significantly affects the mechanical properties of hydrogels. Herein, the controllable permeation of Fe into PAA networks based on the competition between citric acid (CA)-Fe chelation and PAA-Fe coordination guarantees sustained release of Fe, facilitating homogeneous distribution of ionic cross-links and a certain water content.
View Article and Find Full Text PDFMitochondrial DNA B Resour
September 2019
is an important timber plant endemic to southwest of China. In the present study, we sequenced the complete chloroplast genome of and used the data to reveal the species phylogenetic in Lauraceae. The complete chloroplast genome showed a circular genome of 152,730 bp size with 39.
View Article and Find Full Text PDFBackground: Congenital chloride diarrhea (CCD) in a newborn is a rare autosomal recessive disorder with life-threatening complications, requiring early diagnostics and treatment to prevent severe dehydration and infant mortality. SLC26A3 rs386833481 (c.392C>G; p.
View Article and Find Full Text PDFNicotinamide phosphoribosyltransferase (NAMPT) functions in NAD synthesis, apoptosis, and inflammation. Dysregulation of NAMPT has been associated with several inflammatory diseases, including rheumatoid arthritis (RA). The purpose of this study was to investigate NAMPT's role in arthritis using mouse and cellular models.
View Article and Find Full Text PDFAcetaminophen (APAP) is a commonly used analgesic responsible for more than half of acute liver failure cases. Identification of previously unknown genetic risk factors would provide mechanistic insights and novel therapeutic targets for APAP-induced liver injury. This study used a genome-wide CRISPR-Cas9 screen to evaluate genes that are protective against, or cause susceptibility to, APAP-induced liver injury.
View Article and Find Full Text PDFAlthough a deficiency of surfactant protein B (SFTPB) has been associated with lung injury, SFTPB expression has not yet been linked with nicotinamide phosphoribosyltransferase (NAMPT), a potential biomarker of acute lung injury (ALI). The effects of Nampt in the pulmonary epithelial cell on both SFTPB expression and lung inflammation were investigated in a LPS-induced ALI mouse model. Pulmonary epithelial cell-specific knockdown of Nampt gene expression, achieved by the crossing of Nampt gene exon 2 floxed mice with mice expressing epithelial-specific transgene Cre or by the use of epithelial-specific expression of anti-Nampt antibody cDNA, significantly attenuated LPS-induced ALI.
View Article and Find Full Text PDFBackground: Bone degenerative disorders like osteoporosis may be initiated by age-related shifts in anabolic and catabolic responses that control bone homeostasis. Although there are studies suggesting that metabolic changes occur with stem cell differentiation, the molecular mechanisms governing energy metabolism and epigenetic modification are not understood fully. Here we reported the key role of nicotinamide phosphoribosyltransferase (Nampt), which is the rate-limiting enzyme in the salvage pathway of NAD biosynthesis from nicotinamide, in the osteogenic differentiation of bone marrow stromal cells.
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