A Simplified and Turn-on Fluorescence Chemosensor Based on Coumarin Derivative for Detection of Aluminium(III) Ion in Pure Aqueous Solution.

A novel chemosensor L based on coumarin Schiff-base was synthesized and investigated. Sensor L showed remarkable selectivity for Al(3+) in Tris-HCl aqueous buffer solution (pH 7.2), and the selectivity was not affected by the presence of a large excess of other competitive ions.

First and facile enzymatic synthesis of β-fucosyl-containing disaccharide nucleosides through β-galactosidase-catalyzed regioselective glycosylation.

β-Galactosidase from bovine liver was purified to homogeneity. Its molecular mass was estimated to be 54kDa by SDS-PAGE, 60kDa by gel permeation chromatography, and 57kDa by matrix-assisted laser desorption ionization - time of flight tandem mass spectrum. This enzyme displayed the highest catalytic efficiency with p-nitrophenyl β-d-galactopyranoside (Vmax/Km value, 0.

[Detecting ABO blood type of bloodstain with fluorescent antibody method].

Unlabelled: OBJECTIVE To explore the advantage and feasibility of fluorescent antibody method for detection of blood type in biological material.

Methods: According to theory of specific binding of antigen and antibody, at first the anti-A monoclonal antibody (MA) and anti-B MA were labeled with the fluorescent, then fluorescent-labeled antibodies (FLA) were bound with corresponding biological material (such as bloodstain) in the optimum condition, finally the ABO blood type of bloodstain was determined under microscope fluorescent.

Results: The fluorescent antibody method is highly sensitive, accurate and simple.