[Distribution of Typical Resistant Bacteria and Resistance Genes in Source Water of the Middle and Lower Reaches of the Yellow River].

The Yellow River water of an urban area located in the middle and lower reaches of the Yellow River was taken as the research object， in which the seasonal and along-range distribution of total culturable bacteria， typical antibiotic resistant bacteria （amoxicillin resistant bacteria and sulfamethoxazole-resistant bacteria）， and their corresponding typical resistance genes ［-lactam resistance gene （） and sulfamamide resistance genes （ and ）， as well as were investigated. The results showed that the total culturable bacteria， -lactam-resistant bacteria and sulfonamide-resistant bacteria in the Yellow River Basin were significantly affected by temperature and human activities. The composition and quantity of their genera had obvious spatiotemporal distribution characteristics， in which and were dominant in the composition and number of bacteria.

Effect of environmental level of methomyl on hatching, morphology, immunity and development related genes expression in zebrafish (Danio rerio) embryo.

The extensive use of carbamate pesticides has led to a range of environmental and health problems, such as surface and groundwater contamination, and endocrine disorders in organisms. In this study, we focused on examining the effects of toxic exposure to the carbamate pesticide methomyl on the hatching, morphology, immunity and developmental gene expression levels in zebrafish embryos. Four concentrations of methomyl (0, 2, 20, and 200 μg/L) were administered to zebrafish embryos for a period of 96 h.

Bear bile powder ameliorates type 2 diabetes modulation of metabolic profiles, gut microbiota, and metabolites.

Bear bile powder (BBP) is widely used in the clinic and has a hypoglycemic effect, but its mechanism is not clear. In this study, type 2 diabetes mellitus (T2DM) rats induced by a high-sugar and high-fat diet combined with streptozotocin were given BBP, and biochemical indexes, pathological sections, metabonomics, intestinal microbiota (IM) and short-chain fatty acids (SCFAs) were determined. The results showed that BBP could reduce blood glucose, relieve inflammation, insulin resistance, and lipid metabolism disorder, and alleviate tissue damage of the liver, spleen, kidney, and pancreas in T2DM rats.

Bacterial Community Structure and Diversity in the Aqueous Environment of Shihou Lake and its Relationship with Environmental Factors.

Microorganisms are sensitive to changes in the external environment and are often used as indicators to monitor and reflect water quality. Using Illumina MiSeq sequencing, the characteristics of the microbial community in Shihou Lake water at different time points were analyzed and the key environmental factors affecting the bacterial community were identified. The microbial community diversity in Shihou Lake water was rich and showed significant differences over time.

[Chemical constituents and pharmacological action of bile acids from animal:a review].

Bile of animal(mainly chicken, pig, snake, cow, and bear) has long been used as medicine. As the major active components of bile, bile acids mainly include cholic acid, deoxycholic acid, chenodeoxycholic acid, ursodeoxycholic acid, and taurochenodeoxycholic acid. They interact with intestinal microorganisms in enterohepatic circulation, thereby playing an important part in nutrient absorption and allocation, metabolism regulation, and dynamic balance.

SPIKE1 Activates the GTPase ROP6 to Guide the Polarized Growth of Infection Threads in .

In legumes, rhizobia attach to root hair tips and secrete nodulation factor to activate rhizobial infection and nodule organogenesis. Endosymbiotic rhizobia enter nodule primordia via a specialized transcellular compartment known as the infection thread (IT). The IT elongates by polar tip growth, following the path of the migrating nucleus along and within the root hair cell.

Ultrasonographic evaluation of gastric emptying after ingesting carbohydrate-rich drink in young children: A randomized crossover study.

Background: A recent consensus statement in Europe has suggested that the fasting time for clear liquid in children can be shortened to 1 hour before a surgery. However, the study to show that 1-hour fasting time for clear fluids is safe in young children is still lacking. This study aimed to investigate the gastric emptying time for carbohydrate-rich drink and regular 5% glucose solution in children aged 3-7 years.

Effect of Chronic Exposure to Methomyl on Tissue Damage and Apoptosis in Testis of Tilapia (Oreochromis niloticus) and Recovery Pattern.

Tilapia were exposed to 0, 0.2, 2, 20, 200 µg/L methomyl for 30 days, and then transferred to methomyl-free water for 18 days. Caspase-8 in serum, apoptosis rate, microstructure and ultra-microstructure of testis were checked after methomyl exposure and at 18 days after transferring to methomyl-free water.

Effect of methomyl on sex steroid hormone and vitellogenin levels in serum of male tilapia (Oreochromis niloticus) and recovery pattern.

Tilapia were exposed to sub-lethal concentrations of 0, 0.2, 2, 20 or 200 μg/L for 30 days, then transferred to methomyl-free water for 18 days. E , T, 11-KTand VTG in serum were examined.

Responses and recovery pattern of sex steroid hormones in testis of Nile tilapia (Oreochromis niloticus) exposed to sublethal concentration of methomyl.

Tilapia were exposed to sublethal methomyl concentrations of 0, 0.2, 2, 20 or 200 μg/L for 30 days, and then transferred to methomyl-free water for 18 days. The sexual steroid hormones 17β-estradiol (E2), testosterone (T), and 11-ketotestosterone (11-KT) in tilapia testes were examined at 0, 6, 12, 18, 24 and 30 days after methomyl exposure, and at 18 days after fish were transferred to methomyl-free water.

[Simultaneous quantitative analysis of different ceramide species in cells by high-performance liquid chromatography-tandem mass spectrometry].

Objective: To establish a high-performance liquid chromatography-tandem mass spectrometry (HPLC-ESI-MS) method for simultaneous quantitative analysis of different ceramide species in cells.

Methods: The analysis was performed on an Agilent 1290 HPLC system with a ZORBAX Eclipse XDB-C8 (150.0 mm × 2.

Effects of chronic exposure of methomyl on the antioxidant system in liver of Nile tilapia (Oreochromis niloticus).

The chronic effect of methomyl on the antioxidant system in tilapia (Oreochromis niloticus) was investigated. Fish were exposed to sub-lethal concentrations of 0.2, 2, 20 and 200μgL(-1) for 30 days, and then transferred to methomyl-free water for 18 days.

Responses of glutathione-related antioxidant defense system in serum of Nile tilapia (Oreochromis niloticus) exposed to sublethal concentration of methomyl and recovery pattern.

Tilapia were exposed to sublethal concentrations of 0, 0.2, 2, 20, or 200 μg/L for 30 days, and then transferred to methomyl-free water for 18 days. GST, GPx, GR, GSH, and GSSG in tilapia serum were examined at 0, 6, 12, 18, 24, and 30 days after methomyl exposure and at 18 days after transferring to methomyl-free water.

[Analysis of small supernumerary marker chromosome 15q11 in four infertile males].

Objective: To delineate the origins of small supernumerary marker chromosomes (sSMCs) identified in 4 infertile males.

Methods: The sSMCs were analyzed with combined G-banding, N-banding, multiplex ligation-dependent probe amplification (MLPA), fluorescence in situ hybridization (FISH) and single nucleotide polymorphisms array (SNP-array) techniques.

Results: G-banding analysis has suggested a 46,X,-Y,+mar karyotype in all of the 4 cases.

[Responses of soil enzyme activities to re-vegetation in gully Loess Plateau of Northwest China].

In combining field investigation with laboratory analysis, this paper studied the distribution characteristics of soil enzyme activities along the soil profiles and natural slopes with different re-vegetation treatments in gully Loess Plateau, aimed to assess the responses of the soil enzyme activities to re-vegetation. In the study area, the activities of soil urease, invertase and alkaline phosphatase along natural slopes were highly varied, but the activity of soil catalase was in adverse. The profile distribution of the soil enzyme activities varied significantly with vegetation type, and with increasing soil depth, the activities of soil urease, invertase and alkaline phosphatase decreased while the catalase activity increased.

A proximity-dependent surface hybridization strategy for constructing an efficient signal-on electrochemical DNAzyme sensing system.

A proximity-dependent surface hybridization strategy is employed for designing a "signal-on" electrochemical DNAzyme biosensor. By taking advantage of the high sensitivity of the PDSH strategy, and by realizing the enzymatic hydrolysis reaction in a homogenous system with a unimolecular design, the proposed biosensor shows a very high sensitivity to target molecules.

Label-free optical bifunctional oligonucleotide probe for homogeneous amplification detection of disease markers.

Oligonucleotide-based detection schemes that avoid chemical modification possess significant advantages, including simplified design, intrinsic affinity for targets, low cost and ease to extend applications. In this contribution, we developed a label-free self-locked bifunctional oligonucleotide probe (signaling probe) for the detection of different disease markers in parallel. Two signal enhancement techniques based on isothermal circular strand-displacement polymerization reaction, cyclical nucleic acid strand-displacement polymerization (CNDP) and cyclical common (nonnucleic acid) target-displacement polymerization (CCDP), were employed to implement the amplification assay for p53 gene and PDGF-BB, respectively.

A label-free electrochemical immunoassay for IgG detection based on the electron transfer.

In this study, a highly selective, label-free electrochemical immunoassay strategy based on the charge transport through the multilayer films associated with the electrocatalytic reduction of [Fe(CN)(6)](3-) is proposed using human immunoglobulin G (human IgG) as the model analyte. The antibody-antigen complex formed on the sensing interface can efficiently induce change of the surface charge characteristics, the conductivity of multilayer film and/or electron transfer distance, resulting in an immunoreaction signal. The current reduction is proportional to the amount of analyte.

[Quantitative investigation on flocculation and destruction of poly-silicic-ferric (PSF) coagulant].

An inorganic polymer, poly-silicic-ferric (PSF) coagulant was prepared using water glass, FeSO4 x 7H2O and NaClO3 by copolymerization. A comparison of coagulation performance, distribution of residual flocs in finished water and influence of turbulent shear force on the flocs between PSF and polyferric aluminum (PFA) was explored by jar tests and particle counter approach. The results indicate that PSF has superior removal of COD at higher dose to PFA.

Reaction mode between Si and Fe and evaluation of optimal species in poly-silicic-ferric coagulant.

A kind of Fe-polysilicate polymer, poly-silicic-ferric (PSF) coagulant was prepared by co-polymerization (hydroxylation of mixture of Fe3+ and fresh polysilicic acid (PS)), in which PSF0.5, PSF1 or PSF3 denotes Si/Fe molar ratio of 0.5, 1 or 3, respectively.

[Effect of vascular endothelial growth factor 165 gene transfection on repair of bone defect: experiment with rabbits].

Objective: To investigate the influence of vascular endothelial growth factor (VEGF) 165 gene transfection on the repair of bone defect.

Methods: 38 New Zealand rabbits underwent resection of a segment 1 cm in length in bilateral radii filled with absorbable gelatin sponge. Dilated solution of the plasmid pcDNA3.