SARS-CoV-2 and Co-Infection in a Returning Traveler.

Since December 2019, the Coronavirus Disease 2019 (COVID-19) pandemic has become a non-neglectable context for the whole healthcare system. Under the background of COVID-19, the detection and diagnosis of malaria cases are under challenge. Here, we reported a COVID-19 and malaria co-infection traveler who has a long living history in Cameroon.

Identification and molecular typing of Naegleria fowleri from a patient with primary amebic meningoencephalitis in China.

Naegleria fowleri is the only Naegleria spp. known to cause an acute, fulminant, and rapidly fatal central nervous system infection in humans called primary amebic meningoencephalitis (PAM). In 2016, a patient with suspected PAM was found in Zhejiang Province of China.

Genetic diversity analysis of PvCSP and its application in tracking of Plasmodium vivax.

Background: Plasmodium vivax remains a potential cause of morbidity and mortality for people living where it is endemic. Understanding the regional genetic diversity of P. vivax is valuable for studying population dynamics and tracing the origins of parasites.

Genetic diversity of Plasmodium Vivax revealed by the merozoite surface protein-1 icb5-6 fragment.

Background: Plasmodium vivax remains a potential cause of morbidity and mortality for people living in its endemic areas. Understanding the genetic diversity of P. vivax from different regions is valuable for studying population dynamics and tracing the origins of parasites.

[Diagnosis and treatment of a local case of Taenia saginata infection in Zhejiang Province].

Objective: To diagnose and provide treatment for a local case of taenia infection in Zhejiang Province and identify the species of the worm.

Methods: The information of disease onset, clinical feature and therapeutic process was collected and epidemiological investigation was carried out. The anal cellophane swab was used to detect the eggs.

[An Investigation on a Case of Family-clustered Paragonimiasis in Wenzhou City].

Paragonimus infection was reported in a family of 8 members after consuming wine-preserved raw crabs. Seven members developed symptoms of fever, cough, sputum, fatigueness, chest pain, and abdominal pain during 2-3 months after crab feeding, while one member was normal. Serum samples were collected from the 7 members for anti-paragonimus antibody test, with 5 members showing positive, 1 weakly positive and 1 negative.

[Identification of Trypanosoma Species in Rattus flavipectus and R. losea using Microscopy and PCR Method].

Objective: To identify two suspected Trypanosoma species in Rattus found in Zhejiang Province using microscopy and PCR method.

Methods: Blood samples were collected from Rattus losea and R. flavipectus.

[Iditification of five imported cases of Plasmodium ovale wallikeri infection in Zhejiang Province].

Objective: To identify and analyze Plasmodium ovale wallikeri in 5 imported malaria cases, who were detected positive by microscopy and negative by conventional PCR.

Methods: Epidemiological information and blood samples were collected from the five patients. The detection was conducted by microscopy, Rapid Diagnostic Test (RDT) and nested PCR with Plasmodium genus-specific, species-specific and Plasmodium ovale wallikeri-specific primers.

Approaches to the evaluation of malaria elimination at county level: case study in the Yangtze River Delta region.

As the progress on transition from malaria control to malaria elimination in the People's Republic of China (P.R. China), four counties/districts, namely Zhabei District and Songjiang District of Shanghai municipality, and Anji County and Haiyan County of Zhejiang Province, representatives of the Yangtze River Delta region, were included in the pilot project of the national malaria elimination programme in P.

Development and application of an AllGlo probe-based qPCR assay for detecting knockdown resistance (kdr) mutations in Anopheles sinensis.

Background: Anopheles sinensis is one of the most important malaria vectors in China and other Southeast Asian countries. High levels of resistance have been reported in this species due to the long-term use of insecticides, especially pyrethroids, for public health and agricultural purposes. Knockdown resistance (kdr) caused by a single base pair mutation in the gene encoding the sodium channel is strongly associated with pyrethroid insecticide resistance in many Anopheles mosquitoes.

[Tracing investigation and diagnosis of one transfusion-transmitted malaria case in Zhejiang Province].

Objective: To identify the sources of infection and the mode of transmission of a malaria case with unknown origin.

Methods: Clinical data of the case were collected and the epidemiological investigation was conducted. The blood samples of the patient and the suspected infection source (blood donor) were detected by microscopy, rapid diagnostic test strip (RDT) and nested PCR.

[Diagnosis and analysis of the first imported ovale malaria case in Wenzhou City].

The first imported case of Plasmodium ovale infection in Wenzhou City was confirmed by microscopy and PCR test. The patient returned from the People's Republic of Congo to Wenzhou for three and a half months presented a history of fever with chills and rigors on April 30, 2012. The results from peripheral blood smear examination and PCR analysis confirmed a diagnosis of P.

[Species identification in 5 imported cases previously diagnosed as Vivax malaria by parasitological and nested PCR techniques].

Objective: To identify the species of malaria parasites in 5 imported cases previously diagnosed as vivax malaria.

Methods: Epidemiological information and blood samples were collected from five patients who returned from Africa and were diagnosed as vivax malaria. The detection was conducted by microscopy, right VIEW rapid malaria test (RDTs) and nested PCR with Plasmodium genus-specific and species-specific primers.

Sodium hydrosulfide alleviates lung inflammation and cell apoptosis following resuscitated hemorrhagic shock in rats.

Aim: To investigate the protective effects of hydrogen sulfide (H2S) against inflammation, oxidative stress and apoptosis in a rat model of resuscitated hemorrhagic shock.

Methods: Hemorrhagic shock was induced in adult male SD rats by drawing blood from the femoral artery for 10 min. The mean arterial pressure was maintained at 35-40 mmHg for 1.

[Pathogen identification and clinical diagnosis for one case infected with Babesia].

Objective: To identify the pathogen and make diagnosis on a case who was misdiagnosed as malaria.

Methods: Clinical and epidemiological information of the suspected case was collected. Blood samples during hospitalization were collected and examined microscopically.

An exogenous hydrogen sulphide donor, NaHS, inhibits the nuclear factor κB inhibitor kinase/nuclear factor κb inhibitor/nuclear factor-κB signaling pathway and exerts cardioprotective effects in a rat hemorrhagic shock model.

Hemorrhagic shock (HS) is a common condition and leading cause of death in trauma patients universally. Severe inflammatory responses during HS finally lead to multiple-organ failure. Hydrogen sulphide (H₂S) is increasingly recognized as an important signaling molecule with various protective effects.

Evaluation of entropy for monitoring the depth of anesthesia compared with bispectral index: a multicenter clinical trial.

Background: As a new electroencephalogram (EEG) signal processing technique for monitoring the depth of anesthesia, entropy consists of two indices: reaction entropy (RE) and state entropy (SE). Our study compared entropy with classical bispectral index (BIS) in reduction of myoelectrical interference and noxious stimuli with EEG signals.

Methods: Two hundred and eighty patients (ASA I-II, 18-60 years old) undergoing scheduled surgeries from seven medical centers were enrolled.

Exogenous hydrogen sulfide protects against traumatic hemorrhagic shock via attenuation of oxidative stress.

Objective: This study was designed to investigate the protective effects of exogenous hydrogen sulfide (H(2)S) on trauma-hemorrhagic shock (T-H).

Materials And Methods: Forty-eight male Sprague-Dawley rats were anesthetized, while 32 were subjected to both midline laparotomy and hemorrhagic shock (35-40 mmHg for 90 min) by bleeding them from the femoral artery. One hour later, resuscitation was initiated with Ringer lactate.

The effect of ketamine on N-methyl-D-aspartate receptor subunit expression in neonatal rats.

Background And Objective: Ketamine has been widely used in paediatric anaesthesia but its influence on development in infants and toddlers still remains unclear. In order to elucidate the influence of ketamine on brain development in neonatal rats, semiquantitative reverse transcriptase PCR, quantitative reverse transcriptase PCR and immunohistochemistry assays were performed to detect the expression of N-methyl-D-aspartate receptor subtypes expression.

Methods: Seven-day-old rats were divided into two random groups.

[Epidemiological investigation on an outbreak of amoebic dysentery in Jiangshan City of Zhejiang Province].

The investigation was carried out from 15 May to 10 June 2006 among diarrhea patients of two schools and periphery residents in Hecun Town, Jiangshan City. Stool samples were examined for Entamoeba histolytica. Water samples were taken for microbial analysis.

[Evaluation of intervention strategy and measures on the control of intestinal parasitic infections].

Objective: To evaluate the effect of intervention strategy and measures for intestinal parasite control in Zhejiang Province.

Methods: The protective rate (PR) and the index of effectiveness (IE) on the overall prevalence of parasites after and before interventions for intestinal parasitic infections were compared in 30 villages of 10 counties randomly selected as investigation spots.

Results: After the implementation of the interventions in the past decade, the total parasite prevalence declined significantly from 77.