Aspirin intervention before ICU admission reduced the mortality in critically ill patients with acute kidney injury: results from the MIMIC-IV.

Aspirin, with its pleiotropic effects such as anti-inflammatory and anti-platelet aggregation, has been widely used for anti-inflammatory, analgesic, and cardiovascular diseases. However, the association between the use of aspirin before the intensive care unit (ICU) and clinical outcomes in critically ill patients with acute kidney injury (AKI) is unknown. Patients with AKI in this retrospective observational study were selected from the Marketplace for Medical Information in Intensive Care IV (MIMIC-IV).

Effects of Mineralocorticoid Receptor Antagonists for Chronic Kidney Disease: A Systemic Review and Meta-Analysis.

Background: Mineralocorticoid receptor blockade could be a potential approach for the inhibition of chronic kidney disease (CKD) progression. The benefits and harms of different mineralocorticoid receptor antagonists (MRAs) in CKD are inconsistent.

Objectives: The aim of the study was to summarize the benefits and harms of MRAs for CKD patients.

Immune responses of mice inoculated with recombinant Lactobacillus plantarum NC8 expressing the fusion gene HA2 and 3M2e of the influenza virus and protection against different subtypes of influenza virus.

To evaluate the efficiency of preventing pathogenic avian influenza by vaccination with recombinant Lactobacillus plantarum (L. plantarum) that expresses conserved antigens, the mucosal and systemic immune responses in animals vaccinated with recombinant L. plantarum NC8-409-1 (NC8-pSIP409-pgsA'-HA2) and NC8-409-2 (NC8-pSIP409-pgsA'-3M2e-HA2) were evaluated.

[Application of micro-lecture in standardized training of endodontic residents].

Purpose: To apply micro-lecture in standardized training of endodontic residents, in order to improve training quality.

Methods: Twenty endodontic residents were randomly divided into 2 groups, 10 students in each group. One group were taught with micro-lecture while the other group with lecture-based learning (LBL).

[Effects of Porphyromonas endodontalis lipopolysaccharides on the expression of monocyte chemotactic protein-1 in mouse osteoblasts].

Purpose: To investigate the effects of lipopolysaccharide (LPS) extracted from Porphyromonas endodontalis (P.endodontalis) on expression of monocyte chemotactic protein-1 (MCP-1) mRNA and protein in MC3T3-E1 cells and the role of p38 mitogen-activated　protein　kinase (MAPK) and nuclear factor-kB(NF-kB）in the process.

Methods: MC3T3-E1 cells were treated with different concentrations of P.

[Application of PBL combined with SP method in during-course practice of endodontics for undergraduate dental students].

Purpose: To apply problem-based learning (PBL) combined with standardized patients(SP) in during-course practice of endodontics for undergraduate dental students, in order to improve the teaching quality.

Methods: One hundred and four undergraduate dental students of China Medical University School of Stomatology were randomly divided into 2 groups, 52 students in each group. One group were taught with PBL combined with SP while the other group with lecture-based learning (LBL) alone.

[Application of problem-based learning in pre-job training of postgraduate students in department of endodontics].

Purpose: To apply problem-based learning (PBL) in pre-job training of postgraduate students in department of endodontics.

Methods: Thirty master degree postgraduate students of China Medical University were randomly divided into 2 groups, there were 15 students in each group. One group were taught with PBL method while the other group with lecture-based learning (LBL) method.

[Effect of Carisolv on endodontic microleakage].

Purpose: To evaluate the effect of Carisolv on endodontic microleakage by establishing a fluid transport model using sodium nitrite as tracer.

Methods: Seventy extracted teeth were randomly divided into 5 groups, all the root canals were prepared with different chemical reagents, including Carisolv,3% hydrogen peroxide+ 2%tosylchloramide sodium,2.5% sodium hypochlorite,5% sodium hypochlorite(negative control)and distilled water(positive control),post spaces were prepared after cold lateral condensation, the samples were put on the model, concentration of sodium nitrite which penetrated from the coronal to the apical end was measured by diazo reaction at 1,2,4,7,10,15,20,25,30,35,40,45,50,55 and 60 day.

[Hypomethylation of 5'CpG island of insulin-like growth factor binding protein-related protein 1 is associated with its overexpression in colorectal cancer].

Objective: To investigate the methylation status of 5'CpG island of insulin-like growth factor binding protein-related protein 1 (IGFBP-rP1) in colorectal cancer and its relationship with gene expression and clinicopathologic parameters.

Methods: Semi-quantitative reverse transcription-PCR (RT-PCR) was used to detect the expression of IGFBP-rP1 in 46 cases of colorectal cancer and their matched normal mucosa. Methylation-specific PCR (MSP) was applied to evaluate the methylation status of 5'CpG island of IGFBP-rP1.

[Effect of Carisolv on root canal cleanness in chemomechanical preparation].

Purpose: To evaluate the effect of Carisolv as chemical reagent on root canal cleanness in chemomechanical preparation.

Methods: Sixty extracted teeth which were dyed with methylene blue were randomly divided into 3 groups, different chemical reagents were applied in different groups. The optical density of methylene blue in irrigating solution were measured with UV-VIS spectrophotomer after preparation with each file, the root canal cleanness was evaluated under SEM.

[The expression of Staufen gene in colorectal cancer].

To study the expression of differential methylation-associated Staufen gene in colorectal cancer (CRC), we detected the expression of Staufen gene in adenocarcinoma, mucosa adjacent to colorectal cancer (MACC) and corresponding distant normal tissue using RT-PCR and immunohistochemistry. We found that the expression of Staufen gene in MACC and adenocarcinoma of CRC was significantly lower than that in corresponding distal normal tissue at mRNA level (P<0.05.

[Study of B2 gene structure and its expression in colorectal cancer].

Objective: To get a complete cDNA sequence of B2 and evaluate the correlation on structure and expression between B2 and insulin like growth factor binding protein related protein 1 (IGFBP-rP1) in colorectal carcinomas, paired normal tissues, adenomas, tissues adjacent to the tumor, and colorectal carcinoma cell lines.

Methods: 5'RACE (rapid amplification of cDNA end) was applied to get the sequence of the 5' end of B2. Semi-quantitative RT-PCR and immunohistochemistry were used to detect the expression of B2 in colorectal cancer tissues and cell lines (SW480, SW1116, SW620, HCT8, CoLo205 and LoVo).