Repeated implantation failures and infertility in patients are strongly associated with elevated integrin B3 due to endometrial copy number variation.

This study reveals the association between gene copy number variants (CNVs) and integrin expression in endometrial tissue, especially focusing on patients with repeated implantation failure and unexplained infertility. The CNV expression, integrin αVβ3 (by real-time quantitative polymerase chain reaction and protein immunoblotting), and related clinical indicators were investigated by analyzing endometrial samples from 48 patients with repeated implantation failures and 10 patients with infertility and with no history of implantation issues. The results revealed the presence of CNVs in approximately 31.

Development of a mesoporous polypyrrole nanofiber mat for simultaneous detection of multiple mycotoxins in various foods.

Due to health hazards and co-contamination of mycotoxins, efficient separation and detection of multiple mycotoxins in food is highly desirable yet challenging. In this study, we prepared a novel mesoporous polypyrrole nanofiber mat (M-PPy NFM) for extracting multiple mycotoxins from food. The mesoporous effects and multifunctional PPy contribute to higher recovery and purification efficiency of M-PPy NFM for mycotoxins by facilitating hydrogen bonding and π-π interaction.

Optimization and application of high-throughput supported liquid extraction for simultaneous determination of carotenoids and fat-soluble vitamins in serum.

The demand for analysis of carotenoids (CAR) and fat-soluble vitamins (FSV) is continuously expanding, but currently used sample preparation methods either require complicated extraction procedure or large sample volume, let alone the reliability of the results. This study aimed to develop a fast, high-efficient, and high-throughput method based on supported liquid extraction (SLE) for the simultaneous extraction of FSV and CAR from human serum before using high-performance liquid chromatography-diode array detector (HPLC-DAD) analysis. The optimization of SLE parameters was achieved through response surface methodology (RSM) based on the Box-Behnken design (BBD) and included serum-water-extraction solvent ratio and eluent volume.

Decreased expression of CLCA2 and the correlating with immune infiltrates in patients with cervical squamous cell carcinoma: A bioinformatics analysis.

Objective: Calcium-activated chloride channel 2 (CLCA2) is closely related to the invasion, metastasis, and prognosis of some common malignant tumors. The present study aimed to evaluate the role of CLCA2 in cervical squamous cell carcinoma (CESC) using bioinformatics analysis.

Materials And Methods: The mRNA sequencing data and the corresponding clinical data were obtained from Gene Expression Omnibus (GEO) database and The Cancer Genome Atlas (TCGA) database respectively.