[Comparison of bowel function pre and post-sigmoid augmentation cystoplasty in spinal cord injury patients].

Objective: To explore the changes of bowel function in spinal cord injury (SCI) patients undergoing sigmoid augmentation cystoplasty.

Methods: From September 2005 to January 2011, 30 SCI patients undergoing sigmoid augmentation cystoplasty were surveyed by follow-up questionnaires at Beijing Charity hospital and Affiliated Hospital of Nantong University.

Results: Among them, 18 cases (60.

[Interference of invasion and metastasis of colorectal cancer cells by EMS1-siRNA encoded by a recombinant lentiviral vector].

Objective: To explore the effect of Cortactin on invasion and metastasis of human colorectal cancer cells through silencing the expression of Cortactin by transfecting colorectal cancer cells with EMS1-siRNA encoded by a recombinant lentiviral vector.

Methods: EMS1-siRNA lentiviral vector and negative control lentiviral vector were both transfected into HCT8 cells after virus packing, then the expression of Cortactin of the cells was examined by Western blot to determine the inhibited level of Cortactin. The cells were defined as three groups: EMS1-siRNA transfecting cells, negative control transfecting cells and un-transfection cells.

[A pivotal role of cortactin, a CTTN encoding protein, in endocytosis of human colon cancer].

Objective: To explore the role of cortactin in endocytosis of colon cancer cells and clarify the significance of its over-expression in colon cancer tissues.

Methods: Immunohistochemistry and Western blot were employed to detect the expression of cortactin in benign and malignant tissues and cells. Cell endocytosis was examined in cancer cells after siRNA treatment and DNA transfection with plasmid encoding cortactin wild type and domain deletion mutants.