[Relationship of polymorphisms of AhR -1661G/A with GSTP1 -313A/G and susceptibility to endometriosis].

Objective: To investigate the association of polymorphisms of arylhydrocarbon receptor (AhR)-1661G/A with glutathione S-transferase pi (GSTP1) -313A/G and the susceptibility to endometriosis in southern Han Chinese.

Methods: Total of 432 endometriosis patients undergoing laparoscopic or laparotomy surgery matched with 493 patients with fallopian tube ligation, tubal recanalization, laparoscopic hydrotubation, benign ovarian tumor and teratoma surgeries without endometriosis as control group were enrolled in this study. The single nucleotide polymorphism (SNP) of AhR -1661G/A and GSTP1 -313A/G were detected by using a fluorescent quantitative PCR-based high resolution melting (HRM).

[Association of the tumor necrosis factor-alpha -1031T/C and its combination with interleukin-6 -634C/G gene polymorphisms with susceptibility to endometriosis].

Objective: To investigate the association of tumor necrosis factor-alpha (TNF-α) gene promoter region -1031T/C and its combination with interleukin-6 (IL-6) gene promoter region -634C/G single nucleotide polymorphisms (SNP) with the genetic susceptibility to endometriosis.

Methods: Total of 432 endometriosis patients and 499 non-endometriosis women who had received an operation due to tubal ligation, tubal recanalization, laparoscopic hydrotubation, ovarian simple cyst and teratoma were collected and separated into endometriosis group and control group, that all cases were confirmed by operation and pathology. A case-control study was performed in endometriosis and control group to evaluate the association of these SNP with the susceptibility to endometriosis by using a fluorescent quantitative PCR-based high resolution melting (HRM) method.

Chinese medicine for menopausal syndrome: current status, problems and strategies.

The use of Chinese medicine (CM) for the management of: menopausal syndrome is considered effective both at home and abroad, and more and more clinical studies are confirming its efficacy. However, many problems still exit in current studies, such as the standard of CM syndrome differentiation, the design methodology and criteria to assess the quality of clinical trials and the efficacy of interventions. In this paper, the authors present the CM research and treatment strategies for menopausal syndrome with concepts explaining the CM understanding of the mechanism of the disorder.

Prevalence of specific and phylogenetically closely related genotypes in the population of Candida albicans associated with genital candidiasis in China.

Genitourinary candidiasis, which is most frequently caused by Candida albicans, is a common problem worldwide. The pathogenesis of the infection, especially recurrence of the infection, remains to be elucidated. This study analyzed 199 independent Chinese C.

Distribution of Candida albican genotype and Candida species is associated with the severity of vulvovagianl candidiasis.

Objective: To investigate the distribution of pathogenic C.albican genotype and Candida species in association with the severity of vulvovaginal candidiasis (VVC).

Methods: Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) of the internal transcribed spacer analysis was employed to identify the Candida species isolated from the vaginal secretions of 198 patients with acute VVC.

[Association of the IL-6 gene 634C/G polymorphism with susceptibility to endometriosis].

Objective: To investigate the association of interleukin 6 gene (IL-6) promoter region 634C/G (rs1800796) single nucleotide polymorphism (SNP) with the genetic susceptibility to endometriosis (Ems) in south Han Chinese women.

Methods: A case-control study was performed in 432 Ems patients and 499 control women to evaluate the SNP of IL-6 634C/G by using a fluorescent quantitative PCR-based high resolution melting (HRM) method.

Results: There were statistical significances in the IL-6 634C/G alleles, whether or not to carry allele G and genotype distributions between Ems patients and control women (P=0.

[Association of single nucleotide polymorphism in CYP17 and ERα genes with endometriosis risk in southern Chinese women].

Objective: To investigate the association of single nucleotide polymorphisms in cytochrome P450 17 (CYP17) and estrogen receptor alpha (ERα ) genes with the risk of endometriosis among southern Chinese women.

Methods: Two SNPs rs743572 (CYP17 gene 34T/C) and rs9322331 (ERα gene -397T/C) were genotyped by high resolution melting curve in 432 endometriosis patients and 499 matched controls.

Results: There was no significant difference in the genotype frequencies of the two loci between endometriosis patients and the control subjects (P> 0.

[No association of AhR gene 1661G/A and ARNT gene 567G/C polymorphisms with endometriosis in southern Han Chinese women].

Objective: To explore the association between the arylhydrocarbon receptor gene (AhR) 1661G/A or arylhydrocarbon nuclear translocatorgene (ARNT) 567G/C polymorphism and endometriosis in southern Han Chinese women.

Methods: The polymorphisms of AhR gene 1661G/Aand ARNT gene 567G/C in 431 cases of endometriosis and 499 healthy women were genotyped by fluorescence quantitative PCR-based high resolution melting.

Results: The frequencies of genotypes AA, AG, GG and alleles A and G in controls were 12.

[A comprehensive prevention programs on AIDS, HBV and syphilis among pregnant women and couples experienced premarital medical examination].

Objective: To evaluate the effect of comprehensive prevention programs on HIV, HBV and syphilis transmission from mother to child and between premarital couples.

Methods: HIV, HBV and syphilis were screened among pregnant women with interventional measure for infected women; HIV, HBV and syphilis (TP) were screened among premarital couples with medical advice.

Results: The HIV, HBsAg and TP positive rates were 8.

Biased genotype distributions of Candida albicans strains associated with vulvovaginal candidosis and candidal balanoposthitis in China.

Background: Vulvovaginal candidosis (VVC), which is most frequently caused by Candida albicans, is one of the most common vaginal infections and is a common problem worldwide. Despite the fact that extensive epidemiological studies have been performed, what triggers VVC, especially recurrence of the infection, is still uncertain.

Methods: Genotypes of C.

[Effect of gene polymorphism of TNF-beta on the concentration of TNF in serum of patient with endometriosis].

Objective: To determine the polymorphism in +252 site of tumor necrosis factor-beta(TNF-beta) gene in patients with or without endometriosis, to evaluate the levels of TNF-alpha and TNF-beta in the serum with or without endometriosis, to explore the relation between polymorphism of TNF-beta gene and the genetic susceptibility of endometriosis, and to explore the pathogenic mechanism of endometriosis at gene level.

Methods: By polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, polymorphism on +252 site of TNF-beta gene was measured in 82 patients with endometriosis (the endometriosis group) and 80 patients without endometriosis (the control group). With the sandwich-enzyme-linked immunosorbent assay (ELISA), the levels of TNF-alpha and TNF-beta in the serum of the two groups were determined.

[Association of tumor necrosis factors-beta gene polymorphism with endometriosis in women in Guangdong Province].

Objective: To study the polymorphism of +252 site in intron 1 of tumor necrosis factor (TNF)-beta gene in relation to genetic susceptibility of endometriosis.

Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was employed to detect the polymorphism of +252 site in intron 1 of TNF-beta gene in 82 Chinese Han patients with endometriosis in Guangdong Province and 80 Han patients without endometriosis (control group), and the relation between TNF gene polymorphism and the risk of endometriosis was analyzed.

Results: The +252 site of TNF-beta allele and genotype distribution showed significant difference between endometriosis and control groups (Chi2=6.

[Inhibitory effects of integrin av beta3 monoclonal antibody on implanted lesions of endometriosis model of SCID mouse].

Objective: To explore the potential use of integrin av beta3 (Lm609) monoclonal antibody against ectopic lesion and microvessel angiogenesis of human endometriosis xenografts in SCID mice.

Methods: The endometrial tissues from endometriosis patients were inoculated subcutaneously into SCID mouse, which were treated with Lm609, an inhibitor of angiogenesis, twice a week, for 3 weeks. The mice were sacrificed and immunohistochemical staining was employed to determine the microvessel density (MVD) and expression of integrin av beta3 in the lesion tissues.

[Cloning, expression, purification and characterization of human endostatin gene].

Objective: To procure biologically active human endostatin.

Methods: Human endostatin gene was acquired by means of reverse transcriptase (RT)-PCR and cloned into PGEM-T vector with subsequent sequence identification. The gene fragment was inserted into the prokaryotic expression vector pBV220 and transformed into E.

[Expression of hepatocyte growth factor and its receptor c-met gene in the endometrium of women with endometriosis].

Objective: To study the role of hepatocyte growth factor (HGF) and its receptor (c-Met) in the pathogenesis of endometriosis.

Methods: In situ hybridization and immunohistochemistry method was used to examine HGF mRNA and protein expression in eutopic and ectopic endometrium obtained from 54 women in reproductive age with endometriosis confirmed by laparoscopy and histological examination (including 28 cases of stages I/II and 26 of stages III/IV) and from the endometrium of 24 healthy fertile women.

Results: In patients with endometriosis, the positivity rate of HGF/c-Met expression was similar in the eutopic and ectopic endometria, but the number of positive cells and expression intensity were much higher in ectopic endometrium.

[Clinical application of hysteroscopic electroresection in 775 cases].

Objective: To review our experience with hysteroscopic electroresection in the treatment of uterine benign lesions.

Methods: We conducted a retrospective analysis of 775 cases of hysteroscopic surgery in view of the operation time, blood loss, complications and prognosis.

Results: The average operation time was 26.

[Expression of heparanase gene in eutopic and ectopic endometrium of women with endometriosis].

Objective: To study the function of heparanase gene in the pathogenesis of endometriosis.

Methods: In situ hybridization method was used to examine the heparanase mRNA expression in the eutopic and ectopic endometrium of patients with endometriosis (EM group, 23) and the eutopic endometrium of women without endometriosis (control group, 25).

Results: (1) In EM group: The frequency of heparanase gene expression in eutopic was similar to that in ectopic endometrium.

[Establishment of animal models of endometriosis in rhesus monkeys].

Objective: To establish animal models of endometriosis in rhesus monkeys and study the invasion mechanism of endometriosis.

Methods: Five female healthy rhesus monkeys with regular menstrual cycles were selected, of whom 4 were used for endometriosis model establishment and 1 for control. During the period of days 8-15 of menstruation, or 3-5 days after the midcycle estrogen peak, the endometrium was implanted into the pelvic cavity other than the uterus in the experimental group, and in the control group, the greater omentum was implanted instead.

Determination of HGF concentration in serum and peritoneal fluid in women with endometriosis.

Scatter factor (hepatocyte growth factor, SF/HGF) is a multifunctional polypeptide growth factor that plays an important role in tumor proliferation, angiogenesis and invasiveness. The aim of this study was to investigate the role of HGF in endometriosis and their relationship. Peritoneal fluid and serum samples of 126 women of reproductive age receiving laparoscopy (72 with endometriosis and 54 without endometriosis) were obtained and enzyme-linked immunosorbent assay (ELISA) was employed to measure HGF concentrations.

Association of HLA-DPB1 gene with endometriosis in women of Guangdong Province in China.

Objective: To analyze the hereditary susceptibility in patients with endometriosis by way of genotyping of HLA-DPB1 alleles.

Methods: The allelic types of HLA-DPB1 were detected by sequence-based typing (SBT) in 38 patients with endometriosis and 36 healthy women as the control.

Result: Significant differences in the frequency of HLA-DRB1 allele was not observed between endometriotic patients and normal subjects.