Aberrant white matter function and structure in Rhegmatogenous retinal detachment: A study utilizing functional network clustering and TractSeg methods.

Background: Previous studies have documented abnormal functional changes in the visual pathways and gray matter regions related to vision in Rhegmatogenous retinal detachment (RRD) patients. However, the extent of alterations in the functional and structural characteristics of white matter (WM) in these patients remains insufficiently understood.

Methods: In this study, we employed functional clustering networks and TractSeg methodologies to investigate the alterations in WM function and structure among patients with RRD.

Association of triglyceride-glucose index and derived indices with cataract in middle-aged and elderly Americans: NHANES 2005-2008.

Aim: Explore the relationship between the triglyceride-glucose (TyG) index, along with its derivative indices, and the prevalence of cataracts.

Methods: Data from 20,497 participants in the 2005-2008 National Health and Nutrition Examination Survey (NHANES) were compiled. A final total of 4,499 individuals met the eligibility criteria.

[Effect of dual-wavelength laser combined with bioceramics iRoot SP to assist molar root canal treatment].

Purpose: To investigate the effect of dual-wavelength laser combined with bioceramics material iRoot SP on molar root canal treatment.

Methods: A total of 104 patients receiving root canal treatment from January 2020 to June 2023 were selected and divided into control group and experimental group according to random number table method, with 52 cases in each group. The control group was treated with iRoot SP paste, and the experimental group was treated with dual-wavelength laser(Nd:YAG and Er:YAG) combined with iRoot SP paste.

Optimization of the Zhou Tian Formula extraction technology based on AHP-CRITIC method and analysis of transfer efficiency of key components based on HPLC fingerprinting.

Introduction: Zhou Tian Formula (ZTF) is an antidepressant traditional Chinese medicine utilized widely in clinical settings for the treatment of patients with depression. However, shortcomings persist in its extraction technology and quality control.

Objective: This study aimed to propose a methodology for ZTF extraction technology based on the analytic hierarchy process (AHP)-criteria importance through intercriteria correlation (CRITIC) method and to establish a quality control framework for the efficient transfer of index components.

Occurrence of liquid crystal monomers in indoor and outdoor air particle matters (PM): Implications for human exposure indoors.

Liquid crystal monomers (LCMs) are potentially persistent, bioaccumulating, and toxic substances. However, limited data are available on the occurrence of LCMs in indoor and outdoor air particle matter (PM) in residential areas. Herein, residential areas near an e-waste dismantling center (Guiyu Town, Shantou City), as well as areas away from the e-waste site (Jiedong District, Jieyang City) were selected as the sampling areas.

Osteoinduction Evaluation of Fluorinated Hydroxyapatite and Tantalum Composite Coatings on Magnesium Alloys.

Magnesium (Mg) alloys have a wide range of biomaterial applications, but their lack of biocompatibility and osteoinduction property impedes osteointegration. In order to enhance the bioactivity of Mg alloy, a composite coating of fluorinated hydroxyapatite (FHA) and tantalum (Ta) was first developed on the surface of the alloy through thermal synthesis and magnetron sputtering technologies in this study. The samples were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), energy dispersive spectroscopy (EDS) mapping, X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and water contact angle measurement (WCA), which characterized the surface alternation and confirmed the deposition of the target FHA/Ta coating.

Food-grade γ-aminobutyric acid production by immobilized glutamate decarboxylase from Lactobacillus plantarum in rice vinegar and monosodium glutamate system.

Objectives: γ-amino butyric acid (GABA) is a non-protein amino acid, considered a potent bioactive compound. This study focused on biosynthesis of food-grade GABA by immobilized glutamate decarboxylase (GAD) from Lactobacillus plantarum in the rice vinegar and monosodium glutamate (MSG) reaction system.

Results: The gene encoding glutamate decarboxylase (GadB) from L.

Identification of β-conglycinin α' subunit antigenic epitopes destroyed by thermal treatments.

In the present study, phage display technology (PDT) was used to examine β-conglycinin α' subunit epitopes destroyed by heat treatments. The overlapping gene fragments of the α' subunits were amplified using a polymerase chain reaction (PCR) method, and then the recombinant T7 phages containing the target genes were expressed on the phage surface. In addition, an indirect enzyme-linked immunosorbent assay (iELISA) method was adopted to screen the α' subunit epitopes which had been damaged by the heat treatments.

Phosphate regulator PhoP directly and indirectly controls transcription of the erythromycin biosynthesis genes in Saccharopolyspora erythraea.

Background: The choice of phosphate/nitrogen source and their concentrations have been shown to have great influences on antibiotic production. However, the underlying mechanisms responsible for this remain poorly understood.

Results: We show that nutrient-sensing regulator PhoP (phosphate regulator) binds to and upregulates most of genes (ery cluster genes) involved in erythromycin biosynthesis in Saccharopolyspora erythraea, resulting in increase of erythromycin yield.

[Comparison of the effect of three nickel-titanium instruments on molar root canal curvature].

Purpose: To evaluate the influence of ProTaper, Mtwo, and M3 nickel-titanium instruments on root canal curvature during root canal preparation.

Methods: Forty-five molars with root canal therapy were randomly divided into 3 groups. The root canals were prepared by using ProTaper, Mtwo and M3 nickel-titanium instruments.

[Comparison of clinical efficacy of CAD/CAM all -ceramic inlay and polymerid porcelain inlay in restoration of posterior Class II cavity].

Purpose: To compare the clinical efficacy of CAD/CAM all-ceramic inlay and polymerid porcelain inlay in restoring Class II cavity of posterior teeth.

Methods: Ninety-seven patients with 100 posterior teeth of ClassII cavity were recruited in this randomized control trial; Among them, 50 patients were grouped into CAD/CAM all-ceramic inlays and 47 patients were grouped into Ceramage polymerid porcelain inlay. According to the modified USPHS criteria, the incidence of postoperative sensitivity, prosthesis fracture, prosthesis falling off, and edge coloration were evaluated 12 months and 24 months after restoration.

[Influence of age on the effectiveness of revascularization in immature permanent teeth].

Purpose: To evaluate the effect of age on the potential of dental pulp regeneration in young permanent teeth with periapical periodontitis.

Methods: A total of 30 mandibular premolars from 9-18 years old patients with pulp necrosis were divided into 2 groups, group A (younger age group): 9-13 years old, and group B (older age group): 14-18 years old. Revascularization procedures were performed for all patients.

GlnR and PhoP Directly Regulate the Transcription of Genes Encoding Starch-Degrading, Amylolytic Enzymes in Saccharopolyspora erythraea.

Starch-degrading enzymes hydrolyze starch- and starch-derived oligosaccharides to yield glucose. We investigated the transcriptional regulation of genes encoding starch-degrading enzymes in the industrial actinobacterium We observed that most genes encoding amylolytic enzymes (one α-amylase, one glucoamylase, and four α-glucosidases) were regulated by GlnR and PhoP, which are global regulators of nitrogen and phosphate metabolism, respectively. Electrophoretic mobility shift assays and reverse transcription-PCR (RT-PCR) analyses demonstrated that GlnR and PhoP directly interact with their promoter regions and collaboratively or competitively activate their transcription.

Direct and indirect effects of RNA interference against pyridoxal kinase and pyridoxine 5'-phosphate oxidase genes in Bombyx mori.

Vitamin B6 comprises six interconvertible pyridine compounds (vitamers), among which pyridoxal 5'-phosphate is a coenzyme involved in a high diversity of biochemical reactions. Humans and animals obtain B6 vitamers from diet, and synthesize pyridoxal 5'-phosphate by pyridoxal kinase and pyridoxine 5'-phosphate oxidase. Currently, little is known on how pyridoxal 5'-phosphate biosynthesis is regulated, and pyridoxal 5'-phosphate is supplied to meet their requirement in terms of cofactor.

Effect of exogenous hormones on transcription levels of pyridoxal 5'-phosphate biosynthetic enzymes in the silkworm (Bombyx mori).

Vitamin B6 includes 6 pyridine derivatives, among which pyridoxal 5'-phosphate is a coenzyme for over 140 enzymes. Animals acquire their vitamin B6 from food. Through a salvage pathway, pyridoxal 5'-phosphate is synthesized from pyridoxal, pyridoxine or pyridoxamine, in a series of reactions catalyzed by pyridoxal kinase and pyridoxine 5'-phosphate oxidase.

Reciprocal Regulation of GlnR and PhoP in Response to Nitrogen and Phosphate Limitations in Saccharopolyspora erythraea.

Nitrogen and phosphate source sensing, uptake, and assimilation are essential for the growth and development of microorganisms. In this study, we demonstrated that SACE_6965 encodes the phosphate regulator PhoP, which controls the transcription of genes involved in phosphate metabolism in the erythromycin-producing Saccharopolyspora erythraea. We found that PhoP and the nitrogen regulator GlnR both regulate the transcription of glnR as well as other nitrogen metabolism-related genes.

[Preparation and identification of polyclonal antibody against small peptides of beta-tubulin of Toxoplasma gondii].

The amino acid sequences of beta-tubulin from Toxoplasma gondii stains (GT1 and ME49) and human were aligned by ClustalW2 software. Based on the alignment result, the C-terminal peptides of beta-tubulin of T. gondii were artificially synthesized.

Three genes encoding citrate synthases in Saccharopolyspora erythraea are regulated by the global nutrient-sensing regulators GlnR, DasR, and CRP.

Saccharopolyspora erythraea has three citrate synthases encoded by gltA-2, citA, and citA4. Here, we characterized and identified the expression and regulatory properties of these synthases. Three pleiotropic global regulatory proteins of S.

Acetyl coenzyme A synthetase is acetylated on multiple lysine residues by a protein acetyltransferase with a single Gcn5-type N-acetyltransferase (GNAT) domain in Saccharopolyspora erythraea.

Reversible lysine acetylation (RLA) is used by cells of all domains of life to modulate protein function. To date, bacterial acetylation/deacetylation systems have been studied in a few bacteria (e.g.

GlnR-mediated regulation of nitrogen metabolism in the actinomycete Saccharopolyspora erythraea.

Nitrogen source sensing, uptake, and assimilation are central for growth and development of microorganisms which requires the participation of a global control of nitrogen metabolism-associated genes at the transcriptional level. In soil-dwelling antibiotic-producing actinomycetes, this role is played by GlnR, an OmpR family regulator. In this work, we demonstrate that SACE_7101 is the ortholog of actinomycetes' GlnR global regulators in the erythromycin producer Saccharopolyspora erythraea.

[Crystal structure and upconversion emission of Yb3+/Er(3+) -co-doped NaYF4 nanocrystals].

Yb3+/EP(3+) -co-doped cubic NaYF4 and Yb3+/Er3+/Gd(3+) -tri-doped hexagonal NaYF4 nanocrystals were synthesized by a modified coprecipitation method with ethylenediamine tetraacetic acid (EDTA) as chelating agent. The samples' morphology, crystal phase and upconversion emission were measured with transmission electron microscope (TEM), X-ray diffraction patterns (XRD) and upconversion luminescence spectrum. TEM and XRD results showed that the phase transition from cubic to hexagonal was promoted through Gd3+ doping.

Prediction and characterization of small non-coding RNAs related to secondary metabolites in Saccharopolyspora erythraea.

Saccharopolyspora erythraea produces a large number of secondary metabolites with biological activities, including erythromycin. Elucidation of the mechanisms through which the production of these secondary metabolites is regulated may help to identify new strategies for improved biosynthesis of erythromycin. In this paper, we describe the systematic prediction and analysis of small non-coding RNAs (sRNAs) in S.

Development-specific differences in the proteomics of Angiostrongylus cantonensis.

Angiostrongyliasis is an emerging communicable disease. Several different hosts are required to complete the life cycle of Angiostrongylus cantonensis. However, we lack a complete understanding of variability of proteins across different developmental stages and their contribution to parasite survival and progression.

[Research on the application of mid-infrared based quantification of water in oil used by marine-borne machines].

A method that could be used to quantify the concentration of water in oil for marine-borne machine is discussed in the present paper. Water molecule has distinct absorption bands in mid-infrared section in 3773-3509 cm(-1), so it could be applied to the quantification of water in oil. An experimental sample cell was designed to accomplish the experiment for the convenience of sample preparation and mount in measurement, and it was designed to suit for FTIR spectrometer.

Multiple quantitative trait loci Haseman-Elston regression using all markers on the entire genome.

The Haseman-Elston (HE) regression, developed in the 1970s, remains in common use to detect genetic linkage between a quantitative trait and a genetic marker. Although the technique has been improved in a number of ways, it predicts a high rate of false positive quantitative trait locus (QTL) because it is based on a single-QTL model. We have extended the origin HE regression to multi-QTL HE (MQHE) regression, so that all markers across the entire genome can be exploited simultaneously.