Publications by authors named "Li-Jie Shao"
Article Synopsis
- * Experimental methods included various assays and techniques to measure cell viability, autophagy changes, and the expression of key proteins linked to apoptosis.
- * Results indicated that gemcitabine triggers autophagy which reduces the drug's effectiveness, and inhibiting autophagy increased cell death, suggesting combining gemcitabine with autophagic inhibitors could improve treatment outcomes for lung cancer patients.
Hypoxia which commonly exists in solid tumors, leads to cancer cells chemoresistance via provoking adaptive responses including autophagy. Therefore, we sought to evaluate the role of autophagy and hypoxia as well as the underlying mechanism in the cisplatin resistance of lung cancer cells. Our study demonstrated that hypoxia significantly protected A549 and SPC-A1 cells from cisplatin-induced cell death in a Hif-1α- and Hif-2α-dependent manner.
View Article and Find Full Text PDF[Preliminary study on pharmacodynamic evaluation method of Houpo formula particles].
Zhongguo Zhong Yao Za Zhi
April 2014
Article Synopsis
- The study evaluated the pharmacodynamic effects of traditional Chinese medicine (TCM) formula particles compared to traditional decoction using an ulcerative colitis (UC) rat model and specific interventions like Magnoliae Officinalis Cortex.
- Researchers used infrared (IR) fingerprinting to establish similarities between different manufacturers' Magnoliae Officinalis Cortex decoctions and formula particles, while assessing various health indices and changes in UC rats.
- Results indicated that while the Houpo formula particles shared similar pharmacodynamic actions with traditional decoctions, there were no significant differences between particles from different manufacturers, proving the feasibility of the pharmacodynamic evaluation method for TCM formula particles.
Article Synopsis
- Autophagy, a stress response, is being studied in human cancers, specifically focusing on the genes Beclin-1 and LC3, which have not been previously examined in lung cancer.
- In this study, the expression of Beclin-1 and LC3 in 40 primary lung cancer patients was measured through various methods including Real Time PCR and western blotting.
- Results showed lower levels of both Beclin-1 and LC3-II in lung cancer tissues compared to normal tissues, indicating that autophagy might play a role in lung cancer development, independent of patient demographics and cancer stage.