[Prokaryotic expression and purification of human histone-1 and its activity detection].

Aim: To construct the prokaryotic expression vector of human histone-1, obtain the purified GST-H1 protein, and detect its activity.

Methods: Human histone-1 coding region was amplified from human mammary cDNA library, and was inserted into prokaryotic expression vector pGEX-KG. The recombinant plasmid pGEX-KG-H1 was transformed into E.

[Inhibition of the expression of FHL2 by RNA interference].

Aim: To construct the eukaryotic expression vector of small interfering RNA (siRNA) targeting human FHL2 and transfect it into human embryo kidney 293T cells to investigate the silencing effect of FHL2 siRNA on the expression of FHL2 gene.

Methods: Two FHL2 siRNAs were designed and inserted into pSliencer 2.1-U6 neo expression vector.

[Expression of EYA2 in non-small cell lang cancer].

Objective: To identify the expression of Drosophila Eyes Absent Homologue 2 (EYA2) in non-small cell lung cancer (NSCLC) and to investigate its correlation with clinical parameters.

Methods: 59 fresh specimens of lung cancer and paired normal lung tissue were obtained from 59 NSCLC cases treated in the department of thoracic surgery in our hospital from June 2006 to October 2007. Western blotting and immunohistochemistry were used to assay the specimens with goat anti-human EYA2 polyclone antibody.

[Construction of human Egr-1 promoter and its response to ionizing radiation in tumor cells].

Aim: To construct human Egr-1 promoter luciferase reporter system and study its activity induced by ionizing radiation.

Methods: Egr-1 promoter was obtained by human genomic PCR and cloned into pGL3-basic vector. After transfection of recombinant plasmid into human tumor cells, the Egr-1 promoter activity induced by ionizing radiation was detected by luciferase reporter assay.

[Effects of trichostatin A on the interaction between HBx and histone deacetylase protein 1].

Objectives: To study the effects of trichostatin A (TSA) on protein-protein interaction between HBx and histone deacetylase protein 1 (HDAC1).

Methods: Both HBx and HDAC1 expressing vectors were constructed by the method of routine molecular cloning. The expression of HBx and HDAC1 were observed by Western blot assay.

High mobility group box-1 protein acts as a coactivator of nuclear factor of activated T cells-2 in promoting interleukin-2 transcription.

High mobility group box-1 protein, an abundant and conserved constituent of vertebrate nuclei, has recently been reported to be an endogenous immune signal [Rovere-Querini P, Capobianco A, Scaffidi P, Valentinis B, Catalanotti F, Giazzon M, et al. HMGB1 is an endogenous immune adjuvant released by necrotic cells. EMBO Reports 2004;5:825-30].

The antibody preparation and expression of human Pescadillo.

To explore the biological roles of human Pescadillo and investigate its potential effect on tumorigenesis, the cDNA of Pescadillo was fused with that of GST. After purification and elution, the purified GST-Pescadillo fusion protein was obtained, and the antibody against the fusion protein was generated. Endogenous Pescadillo protein was observed to be remarkably induced by estrogen.

[An analysis of the features of HBx protein distributed in liver cells and its expression in E. coli].

Objective: To investigate the features of HBx protein distributed in liver cells and its expression in E. coli.

Methods: The expression vectors encoding the full length HBx and its mutants were constructed by the routine molecular cloning method.

[XBP-1 interacts with estrogen receptor alpha (ERalpha)].

Estrogen receptor alpha (ERalpha) has been a primary target of treatment as well as a prognostic indicator for breast cancer. The level of human X-box binding protein 1 (XBP-1) mRNA was related with that of ERalpha in breast tumors and was over-expressed in some breast tumors. These previous studies suggested that XBP-1 may interact with ERalpha.

[Study of T lymphocyte rDNA transcription activity in chronic benzene poisoning patients].

Objective: To observe the changes in peripheral blood T lymphocyte rDNA transcription activity and to study the significance of immune monitoring for patients with chronic benzene poisoning.

Methods: Venous blood samples were withdrawn from 39 patients with chronic benzene poisoning, 20 patients with malignant disease and 22 healthy controls. Cell culture, argyrophil staining method, I-CLQ cell image analysis system were used in this study.

Spectroscopy behavior of 6-Mercaptopurine, Azathiopurine, and 8-Azaguanine.

A comparative study, luminescence behavior of 6-Mercaptopurine (6-MP), Azathiopurine (BAN), and 8-Azaguanine (8-Azan) have been investigated including the low temperature phosphorescence, the low temperature fluorescence, the room temperature phosphorescence (RTP) and the room temperature fluorescence (RTF). The effect of pH on the luminescence intensity is discussed. Analytical characteristics of RTF and RTP of 6-MP, BAN, and 8-Azan have been studied.

Study on the inclusion complexes of cryptotanshinone with beta-cyclodextrin and hydroxypropyl-beta-cyclodextrin.

The inclusion complexes of beta-cyclodextrin (beta-CD) and HP-beta-cyclodextrin (HP-beta-CD) with a kind of tanshinone, cryptotanshinone (CTan) were investigated by using spectrophotometry. Stable inclusion complexes were established in solution and in solid state and were characterized by UV, IR and 1H NMR spectra, respectively. The optimum pH for inclusion is about 7.

Study on inclusion complex of cyclodextrin with methyl xanthine derivatives by fluorimetry.

The inclusion complexes of beta-cyclodextrin (beta-CD) and HP-beta-cyclodextrin (HP-beta-CD) with caffeine, theophylline and theobromine were investigated by fluorimetry. Various factors affecting the formation of inclusion complexes were discussed in detail including forming time, pH effect and temperature. The results indicate that inclusion process was affected seriously by laying time and pH.

[XBP-1 enhances the transcriptional activity of estrogen receptor alpha].

The estrogen receptor (ERalpha) is a member of a large superfamily of nuclear receptors that regulates the transcription of estrogen-responsive genes. Several recent studies have demonstrated that human X-box binding protein 1 (XBP-1) mRNA expression is associated with ERalpha status in breast tumors. More recently, two forms of XBP-1 were identified due to their unique splicing.

[Spatial orientation interaction mechanism between chondroitin sulfate and azure A].

The spatial orientation interaction mechanism of chondroitin sulfate (CS) and azure A (AA) was studied by spectrometry. The maximum binding number (N = 151) and the binding equilibrium constant (K = 5. 24 x 10(4)) were obtained.

Short term effect of Salvia miltiorrhiza in treating rat acetic acid chronic gastric ulcer and long term effect in preventing recurrence.

AIM:To study the short-term effect of Danshen (Salvia miltiorrhiza) on acetic acid induced chronic gastric ulcer in rats and its long-term effect in preventing recurrence.METHODS:Rats with acetic acid-induced gastric ulcer were treated with Danshen and cimetidine for 30 days.Traditional gastric mucosal auto-radiography and (3)H-TdR incorporation into gastric mucosa in vitro were employed to study the effects of Danshen in rat acetic acid-induced chronic gastric ulcer, including ulcer index (UI), ulcer inhibitory rate (IR) and label rate (LR).