[The mechanism of inhibition by ginkgolide B on interleukin-8 production induced by 4-hydroxynonenal in bronchial epithelial cells].

Objective: 4-Hydroxynonenal (4-HNE) can increase the synthesis of interleukin-8 (IL-8) in bronchial epithelium cells (16HBE). This study was to explore the role of ginkgolide B in inhibiting the synthesis of IL-8 induced by 4-HNE in 16HBE.

Methods: The experiments were divided into 3 groups: a group treated with 4-HNE (10 micromol/L), a group treated with ginkgolide B (100 micromol/L) + 4-HNE (10 micromol/L), and a control group.

[Effects of benzo(a)pyrene on the expression of gamma-glutamate-cysteine ligase: a primary study with rat alveolar epithelial cells].

Objective: To study the effect of benzo(a)pyrene [B(a)P] on the expression of gamma-glutamate-cysteine ligase (gamma-GCS) in rat alveolar epithelium cells (CCL-149 cell line).

Methods: Rat alveolar cells of the line CCL-149 were cultured and exposed to B(a)P of the concentrations of 0, 100, 500, and 5000 microg/L respectively for 24, 36, 48, and 72 hours respectively. Then the A values was measured to observe the influence of B(a)P on the growth of the CCL-149 cells.

[Effect of allitridum on expression of the gamma-glutamyl-cysteine synthetase in rat lung epithelial L2 cells].

Objective: To study the effects of allitridum on the antioxidative capability of rat lung epithelial L2 cells (CCL-149 cell line) by observing the changes of glutathione (GSH), malondialdehyde (MDA) content, and the expressing level of gamma-glutamyl-cysteine synthetase (gamma-GCS) protein.

Methods: The cell viability of CCL-149 cells treated with allitridum was detected by MTT assay. CCL-149 cells were incubated with 0, 0.