CPD-002, a novel VEGFR2 inhibitor, relieves rheumatoid arthritis by reducing angiogenesis through the suppression of the VEGFR2/PI3K/AKT signaling pathway.

Synovial angiogenesis is a key player in the development of rheumatoid arthritis (RA), and anti-angiogenic therapy is considered a promising approach for treating RA. CPD-002 has demonstrated efficacy in suppressing tumor angiogenesis as a VEGFR2 inhibitor, but its specific impacts on RA synovial angiogenesis and possible anti-RA effects need further study. We examined the influences of CPD-002 on the migration and invasion of human umbilical vein endothelial cells (HUVECs) and its impacts on HUVECs' tube formation and vessel sprouting ex vivo.

Metal-injection and interface density engineering induced nickel diselenide with rapid kinetics for high-energy sodium storage.

The key to the innovation of sodium-ion batteries (SIBs) is to find efficient sodium-storage electrode. Here, metal Mo doping of NiSe is proposed by modified electrospinning strategy followed by in situ conversion process. The Mo-NiSe anchoring on hollow carbon nanofibers (HCNFs) would make full use of the multi-channel HCNFs in the inner layer and the active sites of Mo-NiSe in the outer layer, which plays an important role in buffering the volume stress of Na (de)insertion and reducing the adsorption energy barrier of Na.

[Spatial and Temporal Heterogeneity and Ecological Health Evaluation of Diatom Community Based on eDNA].

In recent years, environmental DNA (eDNA) has been widely used in aquatic biodiversity monitoring, and how to establish a river ecological health assessment method based on eDNA has become a hot topic. This study was intended to develop a molecular diatom index based on eDNA to indicate the ecological health status of rivers under the influence of human activities. Firstly, the diatom community composition and structural changes in the Shaying River basin in spring and autumn were monitored through eDNA, and the driving environmental factors of the diatom community were diagnosed.

Recent Strategies for the Immobilization of Therapeutic Enzymes.

Therapeutic enzymes play important roles in modern medicine due to their high affinity and specificity. However, it is very expensive to use them in clinical medicine because of their low stability and bioavailability. To improve the stability and effectiveness of therapeutic enzymes, immobilization techniques have been employed to enhance the applications of therapeutic enzymes in the past few years.

Interfacial Kinetics Regulation of MoS /Cu Se Nanosheets toward Superior High-Rate and Ultralong-Lifespan Sodium-Ion Half/Full Batteries.

Sodium-ion batteries (SIBs) have aroused great attention because of the low cost and environmental benignity of sodium resources. However, practical applications of SIBs are plagued by the sluggish kinetics of sodium ions with large size in the host structure, which results in poor rate performance and rapid capacity decline. Herein, a self-templated approach was developed to synthesize MoS /Cu Se nanosheets with improved interfacial electron- and ion-transfer kinetics.

[Precision of eDNA Metabarcoding Technology for Biodiversity Monitoring of Eukaryotic Phytoplankton in Lakes].

Environmental DNA (eDNA) metabarcoding provides a fast and efficient way to obtain biodiversity information that has been widely used in aquatic biodiversity monitoring and assessment. To facilitate the application of eDNA metabarcoding in China, the accuracy of metabarcoding data needs to be further assessed. Here, the eukaryotic phytoplankton in Dianchi Lake and the northern portion of Fuxian Lake were examined.

Using galactitol dehydrogenase coupled with water-forming NADH oxidase for efficient enzymatic synthesis of L-tagatose.

L-Tagatose, a promising building block in the production of many value-added chemicals, is generally produced by chemical routes with a low yield, which may not meet the increasing demands. Synthesis of l-tagatose by enzymatic oxidation of d-galactitol has not been applied on an industrial scale because of the high cofactor costs and the lack of efficient cofactor regeneration methods. In this work, an efficient and environmentally friendly enzymatic method containing a galactitol dehydrogenase for d-galactitol oxidation and a water-forming NADH oxidase for regeneration of NAD was first designed and used for l-tagatose production.

Ultrathin sulfate-intercalated NiFe-layered double hydroxide nanosheets for efficient electrocatalytic oxygen evolution.

As an important two-dimensional material, layered double hydroxides (LDHs) show considerable potential in electrocatalytic reactions. However, the great thickness of the bulk LDH materials significantly limits their catalytic activity. In this work, we report ultrathin NiFe-LDH nanosheets with sulfate interlayer anions (NiFe(SO)(OH)·7HO) (U-LDH(SO )), which can be synthesized in gram-scale by a simple solvothermal method.

Expression, biochemical characterization, and mutation of a water forming NADH: FMN oxidoreductase from Lactobacillus rhamnosus.

Enzyme-catalyzed cofactor regeneration is a significant approach to avoid large quantities consumption of oxidized cofactor, which is vital in a variety of bioconversion reactions. NADH: FMN oxidoreductase is an ideal regenerating enzyme because innocuous molecular oxygen is required as an oxidant. But the by-product HO limits its further applications at the industrial scale.

Combined cross-linked enzyme aggregates of glycerol dehydrogenase and NADH oxidase for high efficiency in situ NAD regeneration.

Cofactor regeneration is an important method to avoid the consumption of large quantities of oxidized cofactor NAD in enzyme-catalyzed reactions. Herein, glycerol dehydrogenase (GDH) and NADH oxidase preparations by aggregating enzymes with ammonium sulphate followed by cross-linking formed aggregates for effective regeneration of NAD. After optimization, the activity of combi-CLEAs and separate CLEAs mixtures were 950 and 580 U/g, respectively.

In Situ Generation of Bifunctional Fe-Doped MoS Nanocanopies for Efficient Electrocatalytic Water Splitting.

Design and synthesis of non-noble metal electrocatalysts with high activity and durability for the electrolysis of water is of great significance for energy conversion and storage. In this work, we prepared a series of Fe-doped MoS nanomaterials by simple one-pot solvothermal reactions of (NH)MoS with FeCl·6HO. An optimized working electrode of Fe-MoS-5 displayed high hydrogen evolution reaction (HER) activity with a relatively small overpotential of 173 mV to achieve a current density of 10 mA cm in 0.

A hierarchically-assembled Fe-MoS/NiS/nickel foam electrocatalyst for efficient water splitting.

The development of bifunctional non-noble metal electrocatalysts demonstrating high activity and stability for the hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) is of great significance for renewable and clean energy. In this work, we report hierarchically structured integrated Fe-MoS/NiS/NF (NF = nickel foam) materials prepared by a facile in situ solvothermal method, and among them, the Fe-doped MoS was assembled into spine-like nanorods. The optimized electrocatalyst (denoted as Fe-MoS/NiS/NF-2) demonstrated excellent activity and durability for performing the HER and OER in an alkaline electrolyte (pH = 14) with low overpotentials of 130.

Switching the substrate specificity from NADH to NADPH by a single mutation of NADH oxidase from Lactobacillus rhamnosus.

Enzymatic NADP regeneration is a promising approach to produce valuable chemicals under economic conditions. Among all the enzymatic routes, using water-forming NADH oxidase is an ideal one because there is no by-product. However, most NADH oxidases have a low specific activity to NADPH.

A simplified T classification based on the 8th edition of the UICC/AJCC staging system for nasopharyngeal carcinoma.

This study aimed to establish a simplified T classification based on the 8th edition of the Union for International Cancer Control/American Joint Committee on Cancer (UICC/AJCC) staging system for nasopharyngeal carcinoma (NPC). In total, 325 patients with NPC were included in this study. All patients underwent magnetic resonance imaging, and the staging criteria were recorded.

Role of introduced surface cysteine of NADH oxidase from Lactobacillus rhamnosus.

Cysteine, a critical residue for catalytic process but also vulnerable to oxidative damage, was conventionally expressed as a buried catalytic site in most redox enzymes. In the present work, specific surface-exposed sites of a NADH oxidase from Lactobacillus rhamnosus (LrNox) were selected and mutated to cysteine to investigate its effects on catalytic function because LrNox has a buried catalytic cysteine but no surface-exposed one. The results showed that exception of the sites on dimer interface, the activities of LrNox mutants were improved to vary degrees when the polar uncharged and alanine residues were mutated to cysteine.

Large-Scale, Bottom-Up Synthesis of Binary Metal-Organic Framework Nanosheets for Efficient Water Oxidation.

Ultrathin metal-organic framework (MOF) nanosheets (NSs) offer potential for many applications, but the synthetic strategies are largely limited to top-down, low-yield exfoliation methods. Herein, Ni-M-MOF (M=Fe, Al, Co, Mn, Zn, and Cd) NSs are reported with a thickness of only several atomic layers, prepared by a large-scale, bottom-up solvothermal method. The solvent mixture of N,N-dimethylacetamide and water plays key role in controlling the formation of these two-dimensional MOF NSs.

Cloning, expression, characterization and homology modeling of a novel water-forming NADH oxidase from Streptococcus mutans ATCC 25175.

A novel nicotinamide adenine dinucleotide (NADH) oxidase from Streptococcus mutans ATCC 25175 (SmNox) was cloned and overexpressed in Escherichia coli BL21 (DE3). Sequence analysis revealed an open reading frame of 1374bp, capable of encoding a polypeptide of 457 amino acid residues. The molecular mass of the purified SmNox was estimated to be ∼49.

Nanoscale Trimetallic Metal-Organic Frameworks Enable Efficient Oxygen Evolution Electrocatalysis.

Metal-organic frameworks (MOFs) are a class of promising materials for diverse heterogeneous catalysis, but they are usually not directly employed for oxygen evolution electrocatalysis. Most reports focus on using MOFs as templates to in situ create efficient electrocatalysts through annealing. Herein, we prepared a series of Fe/Ni-based trimetallic MOFs (Fe/Ni/Co(Mn)-MIL-53 accordingly to the Material of Institute Lavoisier) by solvothermal synthesis, which can be directly adopted as highly efficient electrocatalysts.

A cuboidal [NiO] cluster as a precursor for recyclable, carbon-supported nickel nanoparticle reduction catalysts.

Cuboidal [NiO] clusters supported by a pyridine alkoxide ligand have been developed. One of these clusters was selected as a precursor for carbon-hosted Ni nanoparticles (NiNPs/C) which were efficient catalysts for the conversion of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) at room temperature.

Synthesis of DMF-protected Au NPs with different size distributions and their catalytic performance in the Ullmann homocoupling of aryl iodides.

DMF-stabilized Au nanoparticles (NPs) with three different particle sizes were prepared by controlling the reaction temperatures and times. In the absence of any additional ligands, these Au NPs showed high catalytic activity in the Ullmann homocoupling of aryl iodides in DMF. The effects of Au particle size on the coupling reaction were investigated by the use of three Au catalysts with mean particle sizes of ca.

Vascularised greater trochanter bone graft, combined free iliac flap and impaction bone grafting for osteonecrosis of the femoral head.

Purpose: To investigate the curative efficacy of osteonecrosis of the femoral head (ONFH) in a hip-preserving operative approach, by grafting a vascularized greater trochanter flap combined with a free iliac flap, in an attempt to seek an innovative approach for patients who suffered middle to late stage ONFH without total hip arthroplasty (THA) surgery.

Method: Our research included a total of 60 patients (66 hips) who accepted hip-preserving surgery by grafting a vascularized greater trochanter flap combined with a free iliac flap which was tightly filled by hammering because of ONFH (most were Association Research Circulation Osseous (ARCO) stage III patients) from January, 2006 to December, 2010. A Harris Hip Score was obtained during follow-ups, evaluating the clinical efficacy, X-rays were taken regularly for image assessing, and the SF-36 scale was used for estimating quality of life.