[Study on the secondary metabolites from marine sponge Phakellia fusca].

Objective: To study the secondary metabolites from marine sponge Phakellia fusca.

Methods: The compounds were isolated by column chromatography over silica gel and purified by Sephadex LH-20 column chromategraphy and preparative TLC. The structures were elucidated by means of physiochemical properties and spectroscopic analysis.

Effect of pulsed electromagnetic field on growth and structure properties of ZnO nanorod arrays.

Well-aligned ZnO nanorod arrays had been prepared by hydrothermal methods assisted with pulsed electromagnetic field (PEMF). The effects of pulsed electromagnetic field on growth and structure properties of ZnO nanorod arrays were studied in detail. XRD and SEM analysis showed ZnO nanorod arrays had bigger length to diameter ratio and better verticality on the substrate.

[Study on the secondary metabolites from the marine sponge Phakellia fusca fungi PF18].

Objective: To study the secondary metabolites from the marine sponge Phakellia fusca epiphytic fungi.

Methods: The compounds were isolated by column chromatography over silica gel and purified by Sephadex LH-20 column chromatography and preparative TLC. The structures were elucidated by means of physiochemical properties and spectroscopic analyses.

New pentahydroxylated steroids from the South China Sea gorgonian Subergorgia suberosa.

Two new pentahydroxylated steroids, (3β,12β,16β,23E)-cholesta-5,23-diene-3,12,16,20,25-pentaol (1) and (3β,12β,16β)-cholesta-5,25(26)-diene-3,12,16,20,24-pentaol (2), were isolated from the EtOH extract of the South China Sea gorgonian Subergorgia suberosa. In addition, four known steroids were detected. The structures of these compounds were established by spectroscopic methods and comparison of their data with those of the related known compounds.

[Expression of telomere repeat binding factor 1 (TRF1) protein in kidney cancer].

Objective: To investigate the expression levels of telomere repeat binding factor 1(TRF1) protein in normal kidney tissue and kidney cancer.

Methods: Specimens of kidney cancer and pericancerous tissues were collected from 32 cases of renal carcinoma. A quantitative Western blotting technique was developed using TRF1 monoclonal antibody to determine the expression level of TRF1 protein in total protein extracts from tissue specimens.

[Expression of human telomere repeat binding factor 1 (TRF1) in acute leukemia cells and its correlation with telomerase activities].

Objective: To study the expression of human telomere repeat binding factor 1 (TRF1) to investigate the correlation of telomerase activity with acute leukemia.

Methods: Leukemic cells were collected from 30 cases of acute leukemia. Realtime quantitative PCR with fluorescence probe hybridization was used to measure expression of TRF1 and hTERT mRNA in leukemic cells.