Intracorporeal versus extracorporeal neobladder in robot-assisted radical cystectomy: perioperative, oncological, and functional outcomes from a single-institutional experience.

Purpose: To compare the perioperative, functional, and oncological outcomes of the intracorporeal neobladder (ICNB) and extracorporeal neobladder (ECNB) technique following robot-assisted radical cystectomy (RARC).

Methods: We reviewed our single-institutional, prospectively collected database for consecutive patients who underwent RARC with an ileum neobladder from July 2016 to February 2023. Patients were divided into 2 groups according to the approach of reconstruction (ICNB or ECNB).

BiFC and FACS-based CRISPR screening revealed that QKI promotes PABPN1 LLPS in colorectal cancer cells.

Protein liquid-liquid phase separation (LLPS), a pivotal phenomenon intricately linked to cellular processes, is regulated by various other proteins. However, there is still a lack of high-throughput methods for screening protein regulators of LLPS in target proteins. Here, we developed a CRISPR/Cas9-based screening method to identify protein phase separation regulators by integrating bimolecular fluorescence complementation (BiFC) and fluorescence-activated cell sorting (FACS).

Radiomic phenotype of peri-coronary adipose tissue as a potential non-invasive imaging tool for detecting atrial fibrillation.

Objectives：: Epicardial adipose tissue (EAT) contribute to atrial fibrillation (AF). We sought to explore the role of FAI (fat attention index), volume, fat radiomic profile (FRP) from peri-coronary artery adipose tissue (PCAT) on coronary computed tomography angiography (CCTA) in determining the presence of AF and differentiating AF types.

Methods: This study enrolled 300 patients underwent CCTA retrospectively and divided them into AF (n = 137) and non-AF groups (n = 163).

Cascade loop of ferroptosis induction and immunotherapy based on metal-phenolic networks for combined therapy of colorectal cancer.

Cancer immunotherapy is the most promising method for tumor therapy, while ferroptosis could activate the immunogenicity of cancer and strengthen the cellular immune response. However, limited by the complex tumor microenvironment, the abundant glutathione (GSH) and low reactive oxygen species (ROS) seriously weaken ferroptosis and the immune response. Herein, the authors report photothermal metal-phenolic networks (MPNs) supplied with buthionine sulfoximine (BSO) by reducing levels of GSH and then trapping the tumor cells in the ferroptosis and immunotherapy cascade loop to eliminate colorectal cancer (CRC).

Machine Learning-Aided Identification of Fecal Extracellular Vesicle microRNA Signatures for Noninvasive Detection of Colorectal Cancer.

Colorectal cancer (CRC) remains a formidable threat to human health, with considerable challenges persisting in its diagnosis, particularly during the early stages of the malignancy. In this study, we elucidated that fecal extracellular vesicle microRNA signatures (FEVOR) could serve as potent noninvasive CRC biomarkers. FEVOR was first revealed by miRNA sequencing, followed by the construction of a CRISPR/Cas13a-based detection platform to interrogate FEVOR expression across a diverse spectrum of clinical cohorts.

PrEP-eligible behaviours and condom use among sexually active older adults in China: Findings from the sexual well-being (SWELL) study.

Objectives: Older adults remain sexually active and at risk of sexually transmitted infections (STIs). This study aimed to explore consistent condom use and pre-exposure prophylaxis (PrEP)-eligible behaviours and their correlates among sexually active older adults in China.

Study Design: Cross-sectional study.

Antifungal activity and active compound identification of Myrothecium spp. against grape anthracnose and gray mold.

Grape anthracnose and gray mold, caused by Elsinoë ampelina and Botrytis cinerea, are devastating fungal diseases resulting in remarkable loss to grapevine production. In this study, the biological control potential of three strains of Myrothecium spp. against E.

Chronic pharyngitis and cervical spondylosis risk: A bidirectional Mendelian randomization study.

Cervical spondylosis (CS) is the most prevalent degenerative disease among the elderly. Chronic pharyngitis (CP) has been reported as a contributing factor to CP. However, the causal relationship between CP and CS has not yet been established.

In vitro simulated digestive properties of Monascus vinegar melanoidins, and cytoprotective functions on Caco-2 cells.

Monascus vinegar melanoidins (LVM), the primary constituents of Monascus vinegar, were subjected to in vitro simulated gastrointestinal digestion to assess their digestibility and bioactivity. While LVM underwent partial degradation, its core melanoidin structure remained intact. Structural characterization and chemical analysis revealed that carbohydrates, proteins, and phenols within LVM were affected during digestion.

Gallic acid protects intervertebral disc cells from ferroptosis and alleviates intervertebral disc degeneration by regulating key factors of oxidative stress.

Background: Intervertebral disc degeneration (IDD) is a chronic degenerative disease and one of the main causes of low back pain (LBP). Currently, there is no effective treatment. Ferroptosis is a cell-regulated process that depends on iron deposition and lipid peroxidation.

Long non-coding RNA NEAT1 promotes colorectal cancer progression via interacting with SIRT1.

Nuclear-enriched abundant transcript 1 (NEAT1), a long noncoding RNA, is found to be significantly dysregulated in different types of cancer, including colorectal cancer (CRC). Nevertheless, there is still much to learn about the precise functions and processes of NEAT1 in the progression of CRC. Using The Cancer Genome Atlas (TCGA) database and 50 CRC specimens from the First Affiliated Hospital of Dali University, we assessed the expression of NEAT1 to determine its clinical impact.

Genome-wide identification and comprehensive analysis of the FtsH gene family in wheat.

Background: The filamentation temperature-sensitive H (FtsH) gene family, which is known to play a critical role in plant growth and development by regulating photosynthesis, chloroplast development, and response to plant stress, has been extensively studied in various species. However, the FtsH gene family in wheat has not been previously documented.

Methods And Results: In this study, 38 TaFtsH gene family members were identified, divided into eight groups and unevenly distributed across various chromosomes.

A dual-modified glucomannan polysaccharide selectively sequesters growth factors for skin tissue repair.

Artificial dermal matrixes (ADMs) are valuable clinical options for treating large soft tissue defects, but their suboptimal bioactivities compared with the real tissue limit their therapeutic potential. For example, glycosaminoglycan (GAG) polysaccharides in the native skin vitally and differentially regulate endogenous growth factors (GFs) to maintain tissue homeostasis. However, the GAG used in the current ADMs has often lost such delicate regulation.

Applying dynamic dyadic systems to explore features of relationship-centered care among Spanish and non-Spanish speaking patients.

Objective: Relationship-centered care (RCC) positions the exchange between patient and provider as central to patient care. Due to limitations in analytical approach, how the relational exchange develops throughout the clinical visit remains unclear. Dynamic dyadic systems (DDS) perspectives overcome these limitations to reveal interdependencies and evolving patterns in turn-taking sequences within dyads.

Cell surface protein-protein interaction profiling for biological network analysis and novel target discovery.

The secretome is composed of cell surface membrane proteins and extracellular secreted proteins that are synthesized via secretory machinery, accounting for approximately one-third of human protein-encoding genes and playing central roles in cellular communication with the external environment. Secretome protein-protein interactions (SPPIs) mediate cell proliferation, apoptosis, and differentiation, as well as stimulus- or cell-specific responses that regulate a diverse range of biological processes. Aberrant SPPIs are associated with diseases including cancer, immune disorders, and illness caused by infectious pathogens.

Causal relationship between apolipoprotein B and risk of atherosclerotic cardiovascular disease: a mendelian randomization analysis.

Background: Atherosclerotic cardiovascular disease (ASCVD) is a major threat to human life and health, and dyslipidemia with elevated low-density lipoprotein cholesterol (LDL-C) is an important risk factor, and in the optimal LDL-C scenario, apolipoprotein B (ApoB) has a more predictive value of ASCVD risk.

Methods: The study is a genome-wide association study (GWAS) based on a European population. A large GWAS dataset for atherosclerotic cardiovascular diseases was targeted, including coronary heart disease (CHD), ischemic stroke (IS), large-artery atherosclerotic stroke (ISL), small-vessel stroke (ISS), and myocardial infarction (MI).

Potential role of P4HB in the tumor microenvironment and its clinical prognostic value: a comprehensive pan-cancer analysis and experimental validation with a focus on KIRC.

Background: Tumor microenvironment (TME) plays a crucial role in tumor growth and metastasis. Exploring biomarkers that are significantly associated with TME can help guide individualized treatment of patients.

Methods: We analyzed the expression and survival of P4HB in pan-cancer through the TCGA database, and verified the protein level of P4HB by the HPA database.

[Expert consensus on clinical application of Dieda Qili Tablets in treatment of acute soft tissue injury].

Dieda Qili Tablets are protected traditional Chinese medicine(TCM) variety and type B medicine included in medical insurance in China, which is widely used in the treatment of acute soft tissue injury in clinical practice and has been recommended by many books. However, there is no evidence-based guideline or consensus to guide the clinical application of Dieda Qili Tablets. With the support of China Association of Chinese Medicine, Wangjing Hospital of China Academy of Chinese Medical Sciences led the 21 Chinese units to follow the guiding ideology of "evidence as the key, consensus as the supplement, and experience as the reference" to compile this consensus.

Research on the material removal mechanism of vibration-assisted nano-scratch on single-crystal GaN by molecular dynamics.

Context: Single-crystal gallium nitride (GaN) is a semiconductor material known for its hardness and brittleness. This research aims to reveal the differences in the micro-mechanisms of material removal during traditional grinding and ultrasonic vibration-assisted grinding and to provide guidance for the high-efficiency, high-quality planarization processing of single-crystal GaN. To achieve this purpose, molecular dynamics (MD) simulation methods were used to establish a model (30 nm × 40 nm × 15 nm) of single-crystal GaN being scratched by a single abrasive grain with and without ultrasonic vibration assistance.

Mechanistic study of the regulation of mitochondrial function by the GPNMB/Nrf2/NF-κB signaling pathway mediated by Quzhi Tang to alleviate chondrocyte senescence.

Ethnopharmacological Relevance: Quzhi Tang (QZT) is a compound formula consisting of six traditional Chinese medicinal herbs. It has achieved good clinical results in the treatment of knee osteoarthritis (KOA), and the potential drug mechanisms involved are worth exploring in depth.

Materials And Methods: Using single-cell transcriptome analysis, this study identified the key target of senescence, GPNMB.

Comprehensive identification of LEA protein family genes and functional analysis of MdLEA60 involved in abiotic stress responses in apple (Malus domestica).

Late embryogenesis abundant (LEA) proteins are important proteins that exists widely in many plants and contribute to physiological processes of plant stress resistance. Despite LEA proteins being identified in many plants, none have been reported in apple (Malus domestica) until this study. In this study, a total of 87 MdLEA proteins were identified in apple, and a comprehensive analysis was conducted to elucidate the functions of MdLEA proteins in response to abiotic stress.

Differential Occupational Health Risks between Methylated PAHs and PAHs: Monitoring 126 PAHs and 6 Oxidative Stress Markers in Paired Serum-Urine Samples.

The hazards of polycyclic aromatic hydrocarbons (PAHs) on occupationally exposed population have been widely acknowledged. However, the occupational exposure risks associated their derivatives, methylated PAHs, remain poorly understood. This study conducted a screen of 126 PAHs and 6 oxidative stress biomarkers (OSBs) in paired serum-urine samples from 110 petrochemical workers to assess the risk associated with different PAHs exposure.

Prediction of ternary alkaline-earth metal Sn(II) and Pb(II) chlorides with potential applications as p-type transparent conductors.

Non-metallic Sn(II) and Pb(II) compounds, particularly those with p-type properties, are essential functional materials due to their notable electronic arrangement and chemical characteristics. The presence of additional Sn(II) and Pb(II) chlorides is suggested by the existence of known Sn(II) and Pb(II) compounds. By utilizing first-principles calculations and swarm intelligence structure search techniques, we have predicted the existence of up to seven new ternary alkaline-earth metal chlorides: ABCl (where A = Sr and B = Sn or Pb) and ABCl (where A = Mg, Ca, or Ba and B = Sn, or A = Ca or Sr and B = Pb).