Unusual depolymerization mechanism of Poly(ethylene terephthalate) by hydrolase 202.

Polyethylene terephthalate (PET) waste significantly contributes to the global plastic crisis, but enzymatic conversion has become an efficient and environmentally friendly strategy to combat it. Therefore, this study explored the Re-face selective depolymerization mechanisms of a novel PET-degradation peptidase, hydrolase 202. Theoretical calculations revealed that the first step, a catalytic triad-assisted nucleophilic attack, is the rate-determining step.

Acupuncture Dosage and Its Correlation with Effectiveness in Patients with Chronic Stable Angina: A Systematic Review and Meta-Analysis of Randomized Controlled Trial.

Objective: This systematic review aimed to compare the efficacy of various acupuncture dosages for Chronic Stable Angina (CSA) using randomized controlled trials (RCTs), addressing the unclear relationship between dosage and effectiveness despite acupuncture's potential.

Methods: We searched eight bibliographic databases from inception to October 31, 2024, evaluating RCTs comparing acupuncture to placebo or standard care for CSA patients, focusing on angina attack frequency as the primary outcome. Studies were categorized into high (HDG), moderate (MDG), and low (LDG) dosage groups based on acupuncture characteristics: the number of acupoints, total sessions, frequency per week, and the need for "Deqi".

Cellulose Based Nano-Scaffolds for Targeted Cancer Therapies: Current Status and Future Perspective.

In the last few years, cellulose has garnered much interest for its application in drug delivery, especially in cancer therapy. It has special properties like biocompatibility, biodegradability, high porosity, and water permeability render it a good candidate for developing efficient carriers for anticancer agents. Cellulose based nanomaterials like cellulose nanofibers, bacterial cellulose, cellulose nanocrystals and microcrystalline cellulose as delivery vehicles for targeted drug delivery to cancer cells are reviewed.

Co-infection of nervous necrosis virus and Vibrio harveyi increased mortality and worsened the disease severity in the orange-spotted grouper (Epinephelus coioides).

Co-infections of different pathogenic microorganisms usually cause complex effects, and receive more attention. Red-grouper nervous necrosis virus (RGNNV) and Vibrio are the common viral and bacterial pathogens of fish, and are often detected simultaneously in diseased fish. However, the understanding of co-infection of RGNNV and Vibrio is still unclear.

The Influence of Work Engagement on Employee Affect and Creativity: Insights from Occupational Mental Health.

Affect plays a pivotal role in shaping employees' work performance and mental health, with growing recognition of its capacity to drive creativity. However, the differential impacts of positive and negative affect on creative performance remain a subject of debate. This study aims to compare the relationships between high- and low-arousal affect, as well as PANA dimensions of affect, and creative performance, emphasizing the mediating role of work engagement from an occupational mental health perspective.

The Impact of Evolving Endometriosis Guidelines on Diagnosis and Observational Health Studies.

Study Question: Do recent changes in European Society of Human Reproduction and Embryology (ESHRE) clinical guidelines result in more comprehensive diagnosis of women with endometriosis?

Summary Answer: The latest shift in clinical guidelines results in diagnosis of more women with endometriosis but current ESHRE diagnostic criteria do not capture a sizable percentage of women with the disease.

What Is Known Already: Historically, laparoscopy was the gold standard for diagnosing endometriosis, a complex gynecological condition marked by a heterogeneous set of symptoms that vary widely among women. More recently, changes in clinical guidelines have shifted to incorporate imaging-based approaches such as transvaginal sonography and magnetic resonance imaging.

Multi-Omics Research on Angina Pectoris: A Novel Perspective.

Angina pectoris (AP), a clinical syndrome characterized by paroxysmal chest pain, is caused by insufficient blood supply to the coronary arteries and sudden temporary myocardial ischemia and hypoxia. Long-term AP typically induces other cardiovascular events, including myocardial infarction and heart failure, posing a serious threat to patient safety. However, AP's complex pathological mechanisms and developmental processes introduce significant challenges in the rapid diagnosis and accurate treatment of its different subtypes, including stable angina pectoris (SAP), unstable angina pectoris (UAP), and variant angina pectoris (VAP).

Acupuncture for angina post percutaneous coronary intervention: a protocol for systematic review and meta-analysis.

Introduction: Ischaemic heart disease (IHD) is a pathological process characterised by a blockage or non-obstructive accumulation of atherosclerotic plaques in the epicardial arteries. Percutaneous coronary intervention (PCI) is widely used in clinical practice to treat IHD. However, angina post PCI (APPCI) impairs quality of life and portends a worse prognosis.

Evaluation of the therapeutic efficacy of renal artery stenting in patients with atherosclerotic renal artery stenosis.

Purpose: It is currently controversial whether renal artery stenting is beneficial for the clinical outcomes of atherosclerotic renal artery stenosis (ARAS) patients. The study aims to evaluate whether blood pressure (BP) and renal function improve in ARAS patients after stenting.

Methods: A retrospective study was conducted on 78 ARAS patients who underwent renal artery stenting at the First Affiliated Hospital of Chongqing Medical University from January 2013 to June 2024.

Atherosclerotic renal artery stenosis, mediating biomarkers, and risk of cardiac among individuals with hypertension: A real-world study.

Background: Atherosclerotic renal artery stenosis (ARAS) is commonly associated with cardiovascular diseases(CVD). Patients with ARAS typically present with cardiac structural and functional abnormalities, and the differences in cardiac structure and function compared to hypertensive patients without ARAS remain to be explored.

Methods: A total of 499 hypertensive patients were included, of whom 134 had ARAS and 365 had no renal artery stenosis (RAS).

The predominant role of IgM in grouper (Epinephelus coioides) mucosal defense against ectoparasitic protozoan infection.

The skin mucosa of fish is exposed to significant challenges from infectious disease agents due to continuous exposure to the aqueous environment. Interestingly, bony fish have evolved to express a unique IgT, which is absent in terrestrials, that appears to play a predominant role in the mucosal-associated lymphoid tissue of the rainbow trout. Nevertheless, in other IgT-producing fish, it is unclear whether IgM or IgT is primarily responsible for protection against infections of cutaneous tissue.

A Graphene-Based Lipid Modulation Nanoplatform for Synergetic Lipid Starvation/Chemo/Photothermal Therapy of Oral Squamous Cell Carcinoma.

Purpose: Chemotherapy is one of the most commonly used treatments for oral squamous cell carcinoma (OSCC), but its use is limited by drug resistance and severe systemic toxicity. To eliminate these side effects and improve anti-tumor efficacy, several therapeutic approaches have been developed for use with chemotherapy. In this study, we developed a graphene-based lipid modulation nanoplatform (NSD) that carries SB-204990, a small molecule inhibitor specific for ATP citrate lyase (ACLY), and doxorubicin (DOX), a chemotherapeutic agent, and the trio enables synergistic treatment of OSCC with lipid starvation, chemotherapy, and photothermal therapy.

Heat Shock Protein 70-2 is Overexpressed in Oral Leukoplakia and Oral Squamous Cell Carcinoma.

Objective: The objective of this study was to assess the correlation between the expression of HSP70-2 and the development of oral potentially malignant disorders (OPMD) and oral squamous cell carcinoma (OSCC). Furthermore, the study evaluates the potential function of HSP70-2 in the pathogenesis of malignant diseases of the oral cavity.

Methods: Using immunohistochemistry, RT-qPCR, Western blot, indirect immunofluorescence, and flow cytometry, the expression of HSP70-2 mRNA and protein in OPMD and OSCC tissues and cells was investigated.

Full on-device manipulation of olefin metathesis for precise manufacturing.

Olefin metathesis, as a powerful metal-catalysed carbon-carbon bond-forming method, has achieved considerable progress in recent years. However, the complexity originating from multicomponent interactions has long impeded a complete mechanistic understanding of olefin metathesis, which hampers further optimization of the reaction. Here, we clarify both productive and hidden degenerate pathways of ring-closing metathesis by focusing on one individual catalyst, using a sensitive single-molecule electrical detection platform.

Sodium Storage Performance and Mechanism of MnO with Different Phase Structures (α, β, γ, δ) as Anode Materials for Sodium-Ion Batteries.

An in-depth understanding of the links between the phase structure and electrochemical property is crucial for the advancement of high-performance anode materials. Herein, the sodium storage performance and mechanisms of MnO with four distinct phase structures (α, β, γ, and δ) as anodes are systematically investigated. Among the four materials, the layered δ-MnO nanoflowers exhibit the best sodium storage performances, characterized by a specific capacity of 303.

A multimodality score strategy for assessing the risk of immune checkpoint inhibitors related cardiotoxicity.

This study aimed to find the association between four common clinical biomarkers and subsequent ICICT, developing a risk scoring strategy to assess the ICICT risk. Three terminals for ICICT were : Terminal 1, cancer therapy-related cardiomyopathies; Terminal 2, myocarditis or heart failure; and Terminal 3, myocarditis, heart failure, myocardial infarction, cerebral infarction, atrial fibrillation, or death. The thresholds were : N-terminal-pro-B-type-natriuretic-peptide ≥ 125 pg/mL, cardiac troponin T ≥ 6 ng/L, high-sensitivity C-reactive protein ≥ 3 mg/L, and coronary artery calcium score > 10 U.

Cryptococcus neoformans osteomyelitis of the radius treatment with antifungal calcium sulphate: A case report and literature review.

Background: is an opportunistic fungal pathogen widely distributed in nature, commonly found in soil and bird droppings such as pigeons. Cryptococcal osteomyelitis is usually found in immunocompromised patients, in an unusual condition Cryptococcus invades the bone. It usually occurs as a part of disseminated infection and is rarely isolated, and isolated cryptococcal osteomyelitis of the radius is rarer.

Pore modulation of single atomic Fe sites for ultrafast Fenton-like chemistry with amplified electron migration oxidation.

The limited interaction between pollutants, oxidants, and the surface catalytic sites of single atom catalysts (SACs) restricts the water decontamination effectiveness. Confining catalytic sites within porous structures enables the localized enrichment of reactants for optimized reaction kinetics, while the specific regulatory mechanisms remain unclear. Herein, SACs with porous modification significantly improves the utilization of peroxymonosulfate (PMS) and pollutant degradation activity.

Efficacy and safety of camrelizumab-based comprehensive treatment for non-small cell lung cancer: a systematic review and meta-analysis.

Background: Many studies show that camrelizumab combination therapy can significantly improve progression-free survival (PFS) and overall survival (OS) in non-small cell lung cancer (NSCLC). However, the time of camrelizumab to market is short, and there is no systematic evaluation of camrelizumab-based comprehensive treatment of NSCLC.

Objectives: To systematically evaluate the efficacy and safety of camrelizumab in comprehensively treating NSCLC.

Role of chitin synthases CHS1 and CHS2 in biosynthesis of the cyst wall of Cryptocaryon irritans.

Cryptocaryon irritans, a protozoan parasite that infects marine fish, is characterized by a complex life cycle that includes a cyst-forming reproductive phase. However, the composition of the cyst wall and mechanism of its formation remain unclear. In this study, we identified chitin as a key component of the cyst wall using calcofluor white and wheat germ agglutinin, with Fourier-transform infrared spectroscopy confirming its β-form structure.

Enzymatic Degradation toward Herbicides: The Case of the Sulfonylureas.

Commercial herbicides, particularly sulfonylureas, are used worldwide and pose a significant challenge to environmental sustainability. The efficient degradation of sulfonylurea herbicides is critical. SulE, an esterase isolated from the bacterial strain S113, shows degradation activity toward sulfonylurea herbicides.